Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   EQI27_RS18410 Genome accession   NZ_CP035163
Coordinates   3532113..3533504 (-) Length   463 a.a.
NCBI ID   WP_041850475.1    Uniprot ID   -
Organism   Bacillus subtilis strain SRCM103923     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3527113..3538504
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EQI27_RS18375 (EQI27_18375) flgL 3527284..3528180 (-) 897 WP_015714797.1 flagellar hook-associated protein FlgL -
  EQI27_RS18380 (EQI27_18380) flgK 3528191..3529714 (-) 1524 WP_003228001.1 flagellar hook-associated protein FlgK -
  EQI27_RS18385 (EQI27_18385) flgN 3529733..3530215 (-) 483 WP_014481048.1 flagellar protein FlgN -
  EQI27_RS18390 (EQI27_18390) flgM 3530231..3530497 (-) 267 WP_014481049.1 flagellar biosynthesis anti-sigma factor FlgM -
  EQI27_RS18395 (EQI27_18395) yvyF 3530578..3530997 (-) 420 WP_003227995.1 TIGR03826 family flagellar region protein -
  EQI27_RS18400 (EQI27_18400) comFC 3531071..3531793 (-) 723 WP_014481051.1 comF operon protein ComFC Machinery gene
  EQI27_RS18405 (EQI27_18405) comFB 3531757..3532053 (-) 297 WP_003227989.1 late competence protein ComFB -
  EQI27_RS18410 (EQI27_18410) comFA 3532113..3533504 (-) 1392 WP_041850475.1 ATP-dependent helicase ComFA Machinery gene
  EQI27_RS18415 (EQI27_18415) fakBA 3533610..3534455 (-) 846 WP_003227986.1 DegV family protein -
  EQI27_RS18420 (EQI27_18420) degU 3534553..3535242 (-) 690 WP_003219701.1 two-component system response regulator DegU Regulator
  EQI27_RS18425 (EQI27_18425) degS 3535325..3536482 (-) 1158 WP_003227983.1 two-component sensor histidine kinase DegS Regulator
  EQI27_RS18430 (EQI27_18430) yvyE 3536699..3537352 (+) 654 WP_003227979.1 YigZ family protein -

Sequence

Protein


Download         Length: 463 a.a.        Molecular weight: 52485.50 Da        Isoelectric Point: 9.9185

>NTDB_id=335146 EQI27_RS18410 WP_041850475.1 3532113..3533504(-) (comFA) [Bacillus subtilis strain SRCM103923]
MNVPVEKNSSFSRELQQTLRSRHLLRTELSFSDEMIEWHIKNGYITAENSISINKRGYRCNRCGQTDQRYYSFYHSSGKN
KLYCRSCVMMGRVSEEVPLYSWEEENAPNWKSIKLTWDGKLSSGQQKAANVLIEAISKKEELLIWAVCGAGKTEMLFPGI
ESALNQGLRVCIATPRTDVVLELAPRLKAAFQGADISALYGGSDDKGRLSPLMISTTHQLLRYKDAIDVMIIDEVDAFPY
SADQTLQFAVQKARKKNSTIVYLSATPPKELKRKALNGQLHSVRIPARHHRKPLPEPRFVWCGNWKKKLNRNKIPPAVKR
WIEFHVKEGRPVFLFVPSVSILEKAAACFRGVHCRTASVHAEDKHRKEKVQQFRDGQLDLLITTTILERGVTVPKVQTGV
LGAESPIFTESALVQIAGRTGRHKEYADGDVIFFHFGKTKSMLDARKHIKEMNELAAKVECTD

Nucleotide


Download         Length: 1392 bp        

>NTDB_id=335146 EQI27_RS18410 WP_041850475.1 3532113..3533504(-) (comFA) [Bacillus subtilis strain SRCM103923]
GTGAATGTGCCAGTTGAAAAAAACAGTTCCTTTTCTAGAGAATTGCAGCAGACGCTTCGAAGCCGTCATTTGCTCAGAAC
TGAGCTCTCATTTTCCGATGAGATGATTGAATGGCATATCAAGAATGGATATATCACTGCTGAAAATTCTATATCCATAA
ATAAACGGGGATATAGATGTAATAGGTGCGGCCAAACTGATCAGCGGTATTATTCTTTTTATCACTCATCTGGAAAGAAT
AAGCTGTATTGCCGTTCCTGTGTCATGATGGGCAGAGTGAGTGAGGAGGTTCCTTTATATTCATGGGAAGAGGAAAATGC
ACCAAACTGGAAGTCAATTAAACTGACATGGGACGGCAAGCTTTCAAGCGGACAGCAAAAAGCCGCCAATGTTTTAATTG
AAGCAATATCAAAAAAAGAAGAGCTCCTCATCTGGGCGGTTTGCGGCGCTGGCAAAACAGAAATGCTGTTTCCTGGTATA
GAATCAGCGTTAAATCAAGGACTGCGTGTATGTATTGCAACACCTCGCACCGATGTTGTATTAGAGCTTGCTCCAAGGCT
CAAGGCTGCCTTTCAGGGTGCTGACATTTCAGCGCTTTACGGAGGAAGCGATGACAAAGGGCGGCTATCTCCGCTTATGA
TTTCCACTACGCATCAGCTTTTGCGATATAAAGATGCAATCGATGTTATGATCATTGATGAAGTTGACGCTTTTCCATAT
TCTGCTGATCAAACCCTTCAATTCGCTGTTCAAAAAGCAAGAAAGAAAAACAGCACCATCGTTTATTTAAGTGCAACACC
TCCTAAAGAATTAAAAAGAAAAGCACTGAACGGACAGTTACATTCAGTTCGCATCCCCGCAAGACACCACCGGAAACCTT
TACCCGAACCGCGCTTTGTATGGTGTGGAAACTGGAAGAAGAAATTAAACCGAAATAAAATTCCGCCAGCGGTGAAAAGA
TGGATAGAGTTTCATGTAAAAGAAGGGAGGCCTGTTTTTTTATTCGTTCCTTCCGTTTCTATTCTGGAAAAGGCTGCTGC
GTGCTTTAGAGGTGTTCATTGCCGAACCGCATCTGTGCACGCGGAAGACAAGCATAGAAAGGAGAAAGTGCAGCAATTCA
GAGATGGTCAGCTCGATCTATTAATCACAACAACAATACTGGAAAGAGGCGTCACAGTCCCCAAGGTGCAAACGGGTGTA
CTAGGAGCGGAATCACCTATCTTTACGGAAAGTGCACTTGTTCAAATTGCAGGAAGAACCGGCCGGCATAAAGAATATGC
GGACGGCGATGTCATTTTCTTTCACTTCGGCAAAACAAAGAGTATGCTCGATGCAAGAAAGCATATAAAAGAAATGAATG
AATTGGCAGCAAAAGTTGAATGTACAGACTAG

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(326-423)

Type III restriction enzyme, res subunit (ResIII)

(120-268)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Bacillus subtilis subsp. subtilis str. 168

97.84

100

0.978

Multiple sequence alignment