Detailed information    

insolico Bioinformatically predicted

Overview

Name   clpE   Type   Regulator
Locus tag   BSN5_RS19240 Genome accession   NC_014976
Coordinates   3649530..3651629 (-) Length   699 a.a.
NCBI ID   WP_015715789.1    Uniprot ID   -
Organism   Bacillus subtilis BSn5     
Function   repress competence development (at the early growth phase) (predicted from homology)   
Competence regulation

Genomic Context

Location: 3644530..3656629
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BSN5_RS19210 (BSn5_18865) spo0E 3644585..3644842 (+) 258 WP_003218598.1 aspartyl-phosphate phosphatase Spo0E -
  BSN5_RS19215 (BSn5_18870) - 3644928..3645359 (+) 432 WP_015715788.1 hypothetical protein -
  BSN5_RS19220 (BSn5_18875) kinD 3645387..3646907 (-) 1521 WP_072557174.1 sporulation kinase KinD -
  BSN5_RS19225 (BSn5_18880) mhqR 3647100..3647537 (+) 438 WP_003232475.1 MarR family transcriptional regulator MhqR -
  BSN5_RS19230 (BSn5_18885) motB 3647577..3648362 (-) 786 WP_003232473.1 flagellar motor protein MotB -
  BSN5_RS19235 (BSn5_18890) motA 3648334..3649146 (-) 813 WP_003238984.1 flagellar motor stator protein MotA -
  BSN5_RS19240 (BSn5_18895) clpE 3649530..3651629 (-) 2100 WP_015715789.1 ATP-dependent protease ATP-binding subunit ClpE Regulator
  BSN5_RS19245 (BSn5_18900) - 3651995..3653038 (+) 1044 WP_015715790.1 membrane protein -
  BSN5_RS19250 (BSn5_18905) queC 3653352..3654011 (+) 660 WP_015715791.1 7-cyano-7-deazaguanine synthase QueC -
  BSN5_RS19255 (BSn5_18910) queD 3654004..3654453 (+) 450 WP_003232462.1 6-carboxytetrahydropterin synthase QueD -
  BSN5_RS19260 (BSn5_18915) queE 3654446..3655177 (+) 732 WP_003232460.1 7-carboxy-7-deazaguanine synthase QueE -
  BSN5_RS19265 (BSn5_18920) queF 3655195..3655692 (+) 498 WP_003218613.1 preQ(1) synthase -
  BSN5_RS19270 (BSn5_18925) ykvN 3656172..3656528 (-) 357 WP_015715792.1 winged helix-turn-helix transcriptional regulator -

Sequence

Protein


Download         Length: 699 a.a.        Molecular weight: 77845.95 Da        Isoelectric Point: 5.1326

>NTDB_id=33511 BSN5_RS19240 WP_015715789.1 3649530..3651629(-) (clpE) [Bacillus subtilis BSn5]
MRCQHCHQNEATIRLNMQINSVHKQMVLCETCYNELTRKPSMSMGPQSFGFPFEQAFQPKEQSAAKQSGKKGLLDELAQN
ITNGAKAGLIDPVIGRDDEVARVIEILNRRNKNNPVLIGEPGVGKTAIAEGLALKIAEGDVPNKLKNKELYLLDVASLVA
NTGIRGQFEERMKQLITELKERKNVILFIDEIHLLVGAGSAEGSMDAGNILKPALARGELQVIGATTLKEYRQIEKDAAL
ERRFQPVMVQEPSIEQAILILQGIKDKYEAYHGVTFSDEAIKACVTLSSRYIQDRHLPDKAIDLLDEAGSKANLLIDELN
DEDAAERLTAIEAEKIKALEEENYELAAKLRDEELALEKKLNSSSAHTAVTVEAEHIQEIVEQKTGIPVGKLQADEQTKM
KELEAKLHERVIGQEAAVQKVAKAVRRSRAGLKSKNRPVGSFLFVGPTGVGKTELSKTLADELFGTKDAIIRLDMSEYME
KHAVSKIIGSPPGYVGHEEAGQLTEKVRRNPYSIVLLDEIEKAHPDVQHMFLQIMEDGRLTDSQGRTVSFKDTVIIMTSN
AGAGEKQTKVGFQSDDSVIEEQTLIDSLSMFFKPEFLNRFDSIIEFRSLEKEHLVKIVSLLLGELEETLAERGISLNVTD
EAKEKIAELGYHPSFGARPLRRTIQEWVEDEMTDLLLDNGEITSFHVILEDDKIKVRAK

Nucleotide


Download         Length: 2100 bp        

>NTDB_id=33511 BSN5_RS19240 WP_015715789.1 3649530..3651629(-) (clpE) [Bacillus subtilis BSn5]
ATGCGTTGTCAACATTGTCATCAAAACGAGGCGACGATTCGCCTTAACATGCAAATAAATTCCGTTCATAAACAGATGGT
TCTTTGTGAAACTTGCTATAACGAACTGACCCGTAAACCTTCAATGAGTATGGGTCCTCAATCTTTCGGATTTCCGTTTG
AACAGGCATTCCAGCCGAAAGAACAGAGCGCAGCAAAACAAAGCGGAAAAAAAGGGCTGCTTGATGAGCTGGCTCAAAAT
ATTACAAACGGTGCTAAAGCCGGTCTCATTGACCCCGTCATCGGCCGTGATGATGAAGTGGCGCGAGTGATCGAAATTCT
AAACCGCCGCAACAAAAACAATCCGGTTCTTATTGGTGAGCCGGGTGTAGGGAAAACTGCCATCGCTGAAGGGCTCGCTT
TAAAAATTGCTGAAGGCGATGTTCCAAACAAACTGAAAAACAAAGAGCTATATTTGCTTGATGTTGCATCCCTTGTTGCA
AACACAGGGATCAGAGGCCAATTTGAGGAGAGAATGAAACAGCTGATCACTGAGTTGAAGGAACGAAAAAATGTCATTCT
GTTCATTGATGAAATTCACCTTCTCGTTGGCGCAGGCTCTGCAGAAGGATCAATGGACGCCGGCAACATTCTCAAACCGG
CCCTAGCCAGAGGCGAACTGCAAGTCATTGGTGCGACAACACTGAAAGAATATCGTCAAATCGAAAAAGATGCCGCGCTG
GAAAGACGTTTTCAGCCTGTCATGGTGCAGGAGCCTTCAATTGAACAGGCTATCCTCATTCTGCAAGGGATTAAAGACAA
ATACGAGGCGTACCATGGCGTAACATTCAGTGATGAAGCAATCAAAGCGTGTGTCACTTTATCATCCCGCTACATTCAGG
ACAGACACCTGCCGGATAAAGCAATTGATTTATTAGATGAAGCAGGTTCAAAAGCCAACCTGTTAATTGATGAACTGAAT
GATGAGGATGCCGCTGAACGCTTAACTGCAATTGAAGCCGAAAAAATAAAAGCCCTGGAAGAAGAAAATTACGAACTGGC
GGCAAAACTCCGTGATGAAGAACTCGCATTGGAGAAAAAACTGAACAGCTCCTCCGCTCATACTGCTGTCACTGTGGAAG
CTGAGCACATTCAGGAAATTGTTGAACAAAAAACAGGCATCCCTGTCGGCAAACTGCAGGCAGACGAACAAACGAAAATG
AAAGAACTCGAAGCAAAACTTCATGAACGCGTAATTGGACAAGAAGCCGCTGTTCAAAAAGTGGCAAAGGCGGTAAGACG
AAGCCGCGCCGGTTTAAAATCCAAAAACAGACCAGTCGGCTCCTTCCTCTTCGTTGGTCCTACCGGCGTAGGGAAAACAG
AGCTTTCTAAAACACTGGCAGATGAATTATTCGGCACAAAAGACGCTATTATCCGACTCGATATGAGCGAATACATGGAG
AAACACGCCGTATCTAAAATTATCGGTTCACCGCCTGGATATGTCGGCCATGAGGAAGCTGGACAATTAACTGAGAAAGT
GCGCCGCAATCCTTACAGCATTGTGTTGCTGGATGAGATTGAAAAAGCACACCCAGACGTTCAGCATATGTTCCTGCAAA
TTATGGAGGATGGCCGTCTGACAGACAGCCAAGGCAGAACCGTAAGCTTCAAAGACACTGTGATCATCATGACAAGTAAT
GCGGGTGCTGGTGAGAAACAAACGAAAGTCGGTTTCCAATCAGATGACAGTGTAATCGAAGAACAAACATTGATTGATTC
ACTGAGCATGTTCTTTAAACCTGAGTTCCTCAACCGTTTTGACAGCATTATTGAGTTCCGCTCATTGGAAAAAGAACATC
TTGTCAAAATCGTCAGCCTTCTTCTTGGAGAACTTGAAGAAACATTGGCTGAACGGGGCATTAGCTTGAATGTGACAGAT
GAAGCGAAAGAAAAAATCGCTGAGCTGGGCTACCACCCTTCATTCGGTGCACGTCCGCTAAGAAGAACCATCCAAGAATG
GGTTGAGGATGAAATGACCGATCTGCTGCTTGATAATGGCGAGATCACAAGTTTTCACGTGATTTTAGAAGATGATAAAA
TTAAAGTGCGAGCGAAATAA

Domains

Predicted by InterProScan.

AAA domain (Cdc48 subfamily) (AAA_2)

(437-603)

ATPase family associated with various cellular activities (AAA) (AAA)

(116-247)

AAA lid domain (AAA_lid_9)

(253-352)

C-terminal, D2-small domain, of ClpB protein (ClpB_D2-small)

(609-679)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  clpE Streptococcus mutans UA159

56.662

100

0.602
  clpC Lactococcus lactis subsp. cremoris KW2

56.557

100

0.592
  clpE Streptococcus pneumoniae TIGR4

54.924

100

0.567
  clpE Streptococcus pneumoniae Rx1

54.924

100

0.567
  clpE Streptococcus pneumoniae D39

54.924

100

0.567
  clpE Streptococcus pneumoniae R6

54.924

100

0.567
  clpC Bacillus subtilis subsp. subtilis str. 168

54.016

90.844

0.491
  clpC Lactococcus lactis subsp. lactis strain DGCC12653

47.861

90.272

0.432
  clpC Streptococcus pneumoniae Rx1

45.942

93.419

0.429
  clpC Streptococcus pneumoniae D39

45.942

93.419

0.429
  clpC Streptococcus thermophilus LMD-9

45.038

93.705

0.422
  clpC Streptococcus pneumoniae TIGR4

46.519

90.415

0.421
  clpC Streptococcus thermophilus LMG 18311

44.58

93.705

0.418
  clpC Streptococcus mutans UA159

46.216

88.841

0.411
  clpA Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819

42.079

86.695

0.365

Multiple sequence alignment