Detailed information    

insolico Bioinformatically predicted

Overview


Name   nucA/comI   Type   Machinery gene
Locus tag   EQH95_RS13850 Genome accession   NZ_CP035162
Coordinates   2569587..2569997 (-) Length   136 a.a.
NCBI ID   WP_009967785.1    Uniprot ID   A0A6I4D881
Organism   Bacillus subtilis strain SRCM103886     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 2562691..2593887 2569587..2569997 within 0


Gene organization within MGE regions


Location: 2562691..2593887
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EQH95_RS13810 (EQH95_13810) yqeH 2562691..2563791 (-) 1101 WP_003229966.1 ribosome biogenesis GTPase YqeH -
  EQH95_RS13815 (EQH95_13815) yqeG 2563795..2564313 (-) 519 WP_003226126.1 YqeG family HAD IIIA-type phosphatase -
  EQH95_RS13820 (EQH95_13820) - 2564675..2564815 (+) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -
  EQH95_RS13825 (EQH95_13825) yqeF 2565121..2565852 (-) 732 WP_003229964.1 SGNH/GDSL hydrolase family protein -
  EQH95_RS13830 (EQH95_13830) cwlH 2566104..2566856 (-) 753 WP_069837642.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  EQH95_RS13835 (EQH95_13835) yqeD 2567043..2567669 (+) 627 WP_014480319.1 TVP38/TMEM64 family protein -
  EQH95_RS13840 (EQH95_13840) gnd 2567688..2568581 (-) 894 WP_069837643.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  EQH95_RS13845 (EQH95_13845) yqeB 2568832..2569554 (+) 723 WP_014480321.1 hypothetical protein -
  EQH95_RS13850 (EQH95_13850) nucA/comI 2569587..2569997 (-) 411 WP_009967785.1 sporulation-specific Dnase NucB Machinery gene
  EQH95_RS13855 (EQH95_13855) sigK 2570193..2570921 (+) 729 WP_013308023.1 RNA polymerase sporulation sigma factor SigK -
  EQH95_RS13860 (EQH95_13860) - 2570921..2571019 (+) 99 WP_031600702.1 hypothetical protein -
  EQH95_RS22965 - 2571016..2571228 (-) 213 Protein_2668 recombinase family protein -
  EQH95_RS13870 (EQH95_13870) fumC 2571447..2572835 (-) 1389 WP_014480325.1 class II fumarate hydratase -
  EQH95_RS13875 (EQH95_13875) - 2573002..2573892 (+) 891 WP_014480326.1 LysR family transcriptional regulator -
  EQH95_RS13880 (EQH95_13880) - 2574833..2575228 (+) 396 WP_014480327.1 VOC family protein -
  EQH95_RS13890 (EQH95_13890) - 2575968..2577095 (-) 1128 WP_014480328.1 Rap family tetratricopeptide repeat protein -
  EQH95_RS23145 (EQH95_13895) - 2577277..2579203 (+) 1927 Protein_2673 T7SS effector LXG polymorphic toxin -
  EQH95_RS13900 (EQH95_13900) - 2579217..2579504 (+) 288 WP_014480331.1 hypothetical protein -
  EQH95_RS13910 (EQH95_13910) - 2579907..2580347 (+) 441 WP_014480332.1 SMI1/KNR4 family protein -
  EQH95_RS13915 (EQH95_13915) - 2580446..2580898 (+) 453 WP_014480333.1 SMI1/KNR4 family protein -
  EQH95_RS13920 (EQH95_13920) cdiI 2580995..2581354 (+) 360 WP_014480334.1 ribonuclease toxin immunity protein CdiI -
  EQH95_RS13925 (EQH95_13925) - 2581459..2581938 (+) 480 WP_224588637.1 hypothetical protein -
  EQH95_RS13930 (EQH95_13930) - 2582242..2582445 (-) 204 WP_123772462.1 hypothetical protein -
  EQH95_RS13935 (EQH95_13935) - 2582525..2582758 (+) 234 WP_224588641.1 hypothetical protein -
  EQH95_RS13940 (EQH95_13940) atxG 2583015..2583592 (+) 578 Protein_2681 suppressor of fused domain protein -
  EQH95_RS13945 (EQH95_13945) - 2583702..2583992 (+) 291 WP_014480337.1 contact-dependent growth inhibition system immunity protein -
  EQH95_RS13955 (EQH95_13955) istA 2584833..2586380 (+) 1548 WP_014480339.1 IS21 family transposase -
  EQH95_RS13960 (EQH95_13960) istB 2586377..2587135 (+) 759 WP_014479891.1 IS21-like element helper ATPase IstB -
  EQH95_RS22985 - 2587453..2587550 (-) 98 Protein_2685 N-acetylmuramoyl-L-alanine amidase -
  EQH95_RS13970 (EQH95_13970) - 2587728..2587814 (+) 87 WP_072592549.1 putative holin-like toxin -
  EQH95_RS23245 (EQH95_13975) - 2588101..2588537 (-) 437 Protein_2687 phage tail tube protein -
  EQH95_RS22715 terS 2588535..2589100 (-) 566 Protein_2688 phage terminase small subunit -
  EQH95_RS13985 (EQH95_13985) - 2589227..2589532 (+) 306 WP_123772463.1 hypothetical protein -
  EQH95_RS23000 - 2589705..2589770 (-) 66 Protein_2690 hypothetical protein -
  EQH95_RS13990 (EQH95_13990) - 2589925..2590404 (-) 480 WP_014480344.1 hypothetical protein -
  EQH95_RS13995 (EQH95_13995) - 2591021..2591287 (+) 267 WP_033881358.1 hypothetical protein -
  EQH95_RS14000 (EQH95_14000) - 2591426..2591578 (-) 153 WP_049832653.1 XtrA/YqaO family protein -
  EQH95_RS14005 (EQH95_14005) - 2591661..2591783 (-) 123 Protein_2694 RusA family crossover junction endodeoxyribonuclease -
  EQH95_RS14010 (EQH95_14010) - 2591746..2591994 (-) 249 Protein_2695 hypothetical protein -
  EQH95_RS23005 - 2592139..2592369 (-) 231 WP_224588644.1 hypothetical protein -
  EQH95_RS14020 (EQH95_14020) - 2592678..2592858 (-) 181 Protein_2697 hypothetical protein -
  EQH95_RS14025 (EQH95_14025) bltR 2593066..2593887 (-) 822 WP_014480349.1 multidrug efflux transcriptional regulator BltR -

Sequence


Protein


Download         Length: 136 a.a.        Molecular weight: 14967.97 Da        Isoelectric Point: 5.1853

>NTDB_id=335051 EQH95_RS13850 WP_009967785.1 2569587..2569997(-) (nucA/comI) [Bacillus subtilis strain SRCM103886]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=335051 EQH95_RS13850 WP_009967785.1 2569587..2569997(-) (nucA/comI) [Bacillus subtilis strain SRCM103886]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGAGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGCGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGCACCAGAGTGCTGTTTA
TTGTGCAGTAG


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB A0A6I4D881

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529


Multiple sequence alignment