Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   EQH88_RS13325 Genome accession   NZ_CP035161
Coordinates   2551453..2551863 (-) Length   136 a.a.
NCBI ID   WP_009967785.1    Uniprot ID   A0A6I4D881
Organism   Bacillus subtilis strain SRCM103862     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2546453..2556863
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EQH88_RS13295 (EQH88_13295) - 2546565..2546705 (+) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -
  EQH88_RS13300 (EQH88_13300) yqeF 2547011..2547742 (-) 732 WP_029726702.1 SGNH/GDSL hydrolase family protein -
  EQH88_RS13305 (EQH88_13305) cwlH 2547994..2548746 (-) 753 WP_029726701.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  EQH88_RS13310 (EQH88_13310) yqeD 2548933..2549559 (+) 627 WP_038427798.1 TVP38/TMEM64 family protein -
  EQH88_RS13315 (EQH88_13315) gnd 2549578..2550446 (-) 869 Protein_2570 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  EQH88_RS13320 (EQH88_13320) yqeB 2550698..2551420 (+) 723 WP_128480810.1 DUF308 domain-containing protein -
  EQH88_RS13325 (EQH88_13325) nucA/comI 2551453..2551863 (-) 411 WP_009967785.1 sporulation-specific Dnase NucB Machinery gene
  EQH88_RS13330 (EQH88_13330) - 2552059..2552406 (+) 348 Protein_2573 sigma-70 family RNA polymerase sigma factor -
  EQH88_RS13335 (EQH88_13335) spoIVCA 2552437..2553897 (-) 1461 WP_223257626.1 site-specific DNA recombinase SpoIVCA -
  EQH88_RS21865 (EQH88_13340) - 2553855..2554033 (-) 179 Protein_2575 hypothetical protein -
  EQH88_RS13345 (EQH88_13345) arsC 2554388..2554807 (-) 420 WP_004398596.1 thioredoxin-dependent arsenate reductase -
  EQH88_RS13350 (EQH88_13350) acr3 2554819..2555859 (-) 1041 WP_004398718.1 arsenite efflux transporter Acr3 -
  EQH88_RS13355 (EQH88_13355) arsK 2555882..2556322 (-) 441 WP_003229954.1 ArsI/CadI family heavy metal resistance metalloenzyme -
  EQH88_RS13360 (EQH88_13360) arsR 2556383..2556700 (-) 318 WP_004399122.1 arsenical resistance operon transcriptional regulator ArsR -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14967.97 Da        Isoelectric Point: 5.1853

>NTDB_id=334973 EQH88_RS13325 WP_009967785.1 2551453..2551863(-) (nucA/comI) [Bacillus subtilis strain SRCM103862]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=334973 EQH88_RS13325 WP_009967785.1 2551453..2551863(-) (nucA/comI) [Bacillus subtilis strain SRCM103862]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAGCAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGGGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGATAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACG
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCTGTCTGCGAGGAAGGCGGCGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGCACCAGAGTGCTGTTTA
TTGTGCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6I4D881

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529

Multiple sequence alignment