Detailed information    

insolico Bioinformatically predicted

Overview

Name   pepF   Type   Regulator
Locus tag   EQI29_RS11520 Genome accession   NZ_CP035136
Coordinates   2251879..2253687 (-) Length   602 a.a.
NCBI ID   WP_002315655.1    Uniprot ID   A0AB74CUB9
Organism   Enterococcus faecium strain SRCM103341     
Function   degradation of XIP; competence shut-off (predicted from homology)   
Competence regulation

Genomic Context

Location: 2246879..2258687
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EQI29_RS11495 (EQI29_11495) - 2247926..2248825 (-) 900 WP_002315659.1 RluA family pseudouridine synthase -
  EQI29_RS11500 (EQI29_11500) - 2248827..2249624 (-) 798 WP_002315658.1 NAD kinase -
  EQI29_RS11505 (EQI29_11505) - 2249599..2250306 (-) 708 WP_002309982.1 GTP pyrophosphokinase family protein -
  EQI29_RS11510 (EQI29_11510) - 2250437..2251012 (+) 576 WP_002315657.1 CYTH domain-containing protein -
  EQI29_RS11515 (EQI29_11515) - 2251130..2251807 (+) 678 WP_002328914.1 ClpXP adapter SpxH family protein -
  EQI29_RS11520 (EQI29_11520) pepF 2251879..2253687 (-) 1809 WP_002315655.1 oligoendopeptidase F Regulator
  EQI29_RS11525 (EQI29_11525) - 2253866..2254864 (-) 999 WP_002328913.1 competence protein CoiA family protein -
  EQI29_RS11530 (EQI29_11530) - 2254962..2255615 (-) 654 WP_002328912.1 adaptor protein MecA -
  EQI29_RS11535 (EQI29_11535) spxA 2255945..2256343 (-) 399 WP_002296384.1 transcriptional regulator SpxA -
  EQI29_RS11540 (EQI29_11540) - 2257108..2257815 (+) 708 WP_002309968.1 HAD family hydrolase -

Sequence

Protein


Download         Length: 602 a.a.        Molecular weight: 69679.05 Da        Isoelectric Point: 4.7044

>NTDB_id=334782 EQI29_RS11520 WP_002315655.1 2251879..2253687(-) (pepF) [Enterococcus faecium strain SRCM103341]
MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEKLKKSEKYKGTLDQGASQFLDAIEFVLSVYRQTEIIYVYAH
LKNDQDTGNTEYQALYARASSLFSKVSEAVSWFEPEILQLSDERIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSADQESL
LAGAGEIFEASSDTFAVLNNADLVFPTIEGENGEKVQLSHGVYGQLLESTDRSVREAAFKGLYSVYEQFRNTFASTLSTH
IKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVAVVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTY
EEAKEKALEALKPMGEEYMTIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHEMGHSVHSY
FTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHYLDGFKGTVFRQTQFAEFEHFMHTEDEKGVP
LTSEYLSDSYGKLNAKYYGPAVEEDPEIKFEWSRIPHFYYNYYVFQYSTGFSAASALAKRILSQEPDALENYLAYLKAGN
SDYPVEVMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL

Nucleotide


Download         Length: 1809 bp        

>NTDB_id=334782 EQI29_RS11520 WP_002315655.1 2251879..2253687(-) (pepF) [Enterococcus faecium strain SRCM103341]
ATGGAAGTAAAGCAGTTGCCAAAACGCGAAGAATTGCCTGAAAATTTAACTTGGGACTTGACCAAGATCTTTTCAAGCGA
CCAAGAATTTGATGAGAAATATCTAGAATTATCAGAAAAGTTAAAAAAATCTGAAAAATACAAAGGAACACTTGATCAAG
GGGCTTCTCAATTTCTTGATGCGATTGAATTTGTGTTGAGTGTTTATCGCCAAACTGAAATCATTTATGTCTATGCGCAC
CTTAAAAACGATCAAGATACTGGGAATACAGAATATCAAGCGCTTTATGCAAGAGCAAGTAGTTTGTTTTCAAAGGTTAG
TGAAGCTGTTTCCTGGTTTGAACCAGAAATATTGCAGTTATCAGATGAACGGATTTGGCAATATTTCAAAGAAGAACCGA
AGTTAGAAGTCTATCGTCACTATATCCAGCAAATAGTAGATAATCGTGCCCATGTTTTATCTGCCGATCAGGAATCGCTT
CTTGCAGGAGCAGGTGAAATCTTTGAGGCTTCAAGTGATACATTTGCTGTTTTGAATAATGCAGATTTAGTTTTCCCAAC
GATCGAAGGAGAGAATGGTGAAAAAGTCCAATTATCTCATGGCGTGTACGGTCAGTTGCTAGAAAGCACGGATCGAAGTG
TTCGCGAAGCCGCATTTAAAGGGTTGTACAGTGTTTATGAACAATTTAGAAATACGTTTGCTTCTACTTTAAGTACGCAT
ATAAAAGGACATAATTTTAAAGCAAAAGTTCGTAATTACAGCTCTGCCAGAGAAGCATCTTTGAGCAATAATCATATTCC
TGAAAGTGTATACGACACTTTGGTAGCCGTGGTAAACAAGCATTTGCCTTTGCTGCATCGATATATGGAATTACGGAAAC
GTTTATTAGAAGTGGAAAAACTGCACATGTACGATCTTTATACACCGGTCTTAGGAGAAGCTCCAATCACCTTTACGTAT
GAAGAAGCAAAAGAGAAAGCTTTGGAAGCACTGAAGCCAATGGGTGAAGAGTACATGACTATTGTAGAAAAAGCATTCTC
TGAACGTTGGATCGATGTTGTCGAAAATAAAGGAAAACGAAGTGGTGCCTATTCTTCGGGAAGCTATGACACAAATCCAT
ATATTTTATTGAATTGGCATGATACATTGGATCAGCTGTTTACGCTTGTCCACGAAATGGGACATAGTGTCCACAGTTAT
TTCACACGGTCGAACCAGCCTTATGTATACGGTGACTACTCTATCTTTTTAGCAGAAATTGCTTCGACAACGAACGAAAA
TATCCTAACAGAATATTTATTGGAAACAGAAAAAGACCCTCGTGTCCGGGCTTATGTGCTCAACCATTACTTGGATGGAT
TTAAAGGAACAGTTTTCCGCCAGACACAATTTGCTGAATTCGAGCACTTCATGCATACGGAAGATGAAAAAGGTGTGCCA
TTGACTAGTGAATATCTAAGTGATAGTTATGGTAAATTGAATGCAAAATATTATGGTCCGGCAGTCGAAGAAGATCCAGA
AATCAAATTTGAATGGTCACGGATCCCGCATTTTTACTATAATTACTATGTCTTCCAATACTCGACTGGTTTTTCTGCTG
CTTCAGCGTTGGCGAAAAGAATACTCAGTCAGGAACCAGATGCGCTAGAGAACTATTTGGCTTATCTAAAAGCTGGTAAC
AGTGATTATCCTGTTGAAGTGATGAAAAAAGCAGGAGTAGATATGACACAGGCTGCGTATATCGAAGATGCAATGTCGAT
GTTTGAACAACGACTAAACGAATTAGAAGAATTGATTGATCGTTTATAA

Domains

Predicted by InterproScan.

Oligopeptidase F (Peptidase_M3_N)

(116-185)

Peptidase family M3 (Peptidase_M3)

(206-586)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pepF Streptococcus salivarius strain HSISS4

55.649

98.505

0.548

Multiple sequence alignment