Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   EPY81_RS06855 Genome accession   NZ_CP035109
Coordinates   1424598..1425398 (+) Length   266 a.a.
NCBI ID   WP_002114699.1    Uniprot ID   -
Organism   Acinetobacter pittii strain NQ-003     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1419598..1430398
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EPY81_RS06830 (EPY81_06885) - 1420514..1421803 (+) 1290 WP_019767998.1 glycosyltransferase family 4 protein -
  EPY81_RS06835 (EPY81_06890) - 1421828..1422379 (+) 552 WP_005079131.1 phosphatase PAP2 family protein -
  EPY81_RS06840 (EPY81_06895) iscX 1422479..1422679 (+) 201 WP_002115051.1 Fe-S cluster assembly protein IscX -
  EPY81_RS06845 (EPY81_06900) ndk 1422793..1423224 (+) 432 WP_000963851.1 nucleoside-diphosphate kinase -
  EPY81_RS06850 (EPY81_06905) rlmN 1423348..1424583 (+) 1236 WP_002115097.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  EPY81_RS06855 (EPY81_06910) pilF 1424598..1425398 (+) 801 WP_002114699.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  EPY81_RS06860 (EPY81_06915) - 1425389..1426180 (+) 792 WP_002114935.1 helix-turn-helix domain-containing protein -
  EPY81_RS06865 (EPY81_06920) ispG 1426202..1427317 (+) 1116 WP_002115078.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  EPY81_RS06870 (EPY81_06925) hisS 1427314..1428606 (+) 1293 WP_002114994.1 histidine--tRNA ligase -
  EPY81_RS06875 (EPY81_06930) - 1428635..1429333 (+) 699 WP_161511766.1 YfgM family protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30114.38 Da        Isoelectric Point: 9.0417

>NTDB_id=334565 EPY81_RS06855 WP_002114699.1 1424598..1425398(+) (pilF) [Acinetobacter pittii strain NQ-003]
MSIPKLKTVLCMGIAVAFLVSGCQTTHTQKKDPEKAVKVRTQLAAEHIRSGDLDSAKRALDQALSVDSRDATANMMMGIL
LQQEGSKPNLEKAEHYFKRAISSEPDNAQAHNNYGTYLYQMERYNDAVEQFRIAGATLGYDQRYQALENLGRIYLKLGDV
ANAEKTFKQALLANRDSYISMLELAEIFYLQQQIPAATQMYEQYVRTVGQKNQGARALWIGVRVARANADKMGMQVLVNQ
LRALFPESPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=334565 EPY81_RS06855 WP_002114699.1 1424598..1425398(+) (pilF) [Acinetobacter pittii strain NQ-003]
TTGAGTATTCCAAAGTTAAAAACGGTTTTATGTATGGGGATTGCAGTTGCATTTCTGGTAAGTGGTTGCCAGACAACGCA
TACGCAAAAAAAAGACCCTGAGAAAGCGGTAAAGGTGCGTACACAACTTGCTGCTGAACATATCCGTTCGGGTGACTTGG
ACTCAGCAAAGCGAGCTCTTGATCAAGCTTTAAGTGTAGATAGCCGCGATGCTACTGCAAATATGATGATGGGTATTTTG
CTACAGCAAGAAGGTAGCAAACCCAATTTAGAAAAAGCAGAACATTATTTTAAAAGAGCAATTTCATCTGAACCGGATAA
TGCTCAAGCACATAATAATTATGGTACTTATTTATATCAAATGGAGCGTTATAATGATGCGGTTGAGCAATTTCGTATTG
CTGGCGCAACATTAGGTTATGACCAACGCTATCAGGCATTAGAAAATCTGGGGCGTATTTATTTAAAATTGGGTGATGTT
GCGAATGCTGAAAAAACATTCAAACAAGCACTCCTTGCTAACCGCGACTCTTATATTTCAATGTTAGAGTTAGCGGAAAT
TTTTTATTTGCAGCAGCAGATTCCGGCTGCTACACAAATGTATGAGCAATATGTTCGTACAGTGGGGCAGAAAAATCAGG
GTGCAAGAGCACTTTGGATTGGTGTAAGAGTGGCTCGAGCCAATGCGGATAAAATGGGAATGCAAGTGTTAGTAAACCAG
TTACGTGCTTTGTTCCCGGAAAGTCCAGAATATCAACGTTATTTGCAATTACAGTATAGTACTGAGGCCGTATGGAAATA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_16)

(89-134)

Tetratricopeptide repeat (TPR_16)

(149-206)

Tetratricopeptide repeat (TPR_8)

(39-68)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

93.985

100

0.94
  pilF Acinetobacter baylyi ADP1

76.154

97.744

0.744

Multiple sequence alignment