Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   FYA19_RS18610 Genome accession   NZ_CP043183
Coordinates   3780499..3780939 (+) Length   146 a.a.
NCBI ID   WP_000360904.1    Uniprot ID   P36647
Organism   Escherichia coli O16:H48 strain PG20180172     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3775499..3785939
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FYA19_RS18590 (FYA19_18590) aroP 3776497..3777870 (+) 1374 WP_000969915.1 aromatic amino acid transporter AroP -
  FYA19_RS18595 (FYA19_18595) ampE 3777913..3778767 (-) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  FYA19_RS18600 (FYA19_18600) ampD 3778764..3779315 (-) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  FYA19_RS18605 (FYA19_18605) nadC 3779403..3780296 (+) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  FYA19_RS18610 (FYA19_18610) pilA 3780499..3780939 (+) 441 WP_000360904.1 prepilin peptidase-dependent pilin Machinery gene
  FYA19_RS18615 (FYA19_18615) pilB 3780949..3782334 (+) 1386 WP_001025145.1 type II secretion system protein GspE Machinery gene
  FYA19_RS18620 (FYA19_18620) hofC 3782324..3783526 (+) 1203 WP_000157266.1 protein transport protein HofC -
  FYA19_RS18625 (FYA19_18625) guaC 3783561..3784604 (-) 1044 WP_001217338.1 GMP reductase -
  FYA19_RS23065 - 3784760..3784804 (-) 45 WP_120795372.1 protein YacM -
  FYA19_RS18630 (FYA19_18630) coaE 3784829..3785449 (+) 621 WP_001269520.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15621.75 Da        Isoelectric Point: 4.3938

>NTDB_id=334527 FYA19_RS18610 WP_000360904.1 3780499..3780939(+) (pilA) [Escherichia coli O16:H48 strain PG20180172]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=334527 FYA19_RS18610 WP_000360904.1 3780499..3780939(+) (pilA) [Escherichia coli O16:H48 strain PG20180172]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P36647

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363

Multiple sequence alignment