Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   FYA20_RS10860 Genome accession   NZ_CP043181
Coordinates   2237766..2239262 (-) Length   498 a.a.
NCBI ID   WP_001040641.1    Uniprot ID   -
Organism   Escherichia coli O2:H6 strain PG20180057     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2232766..2244262
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FYA20_RS10835 (FYA20_10835) panM 2233000..2233383 (+) 384 WP_000778808.1 aspartate 1-decarboxylase autocleavage activator PanM -
  FYA20_RS10840 (FYA20_10840) livJ 2233571..2234674 (-) 1104 WP_001350438.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
  FYA20_RS10845 (FYA20_10845) rpoH 2234945..2235799 (-) 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
  FYA20_RS10850 (FYA20_10850) ftsX 2236044..2237102 (-) 1059 WP_001042003.1 permease-like cell division protein FtsX -
  FYA20_RS10855 (FYA20_10855) ftsE 2237095..2237763 (-) 669 WP_000617723.1 cell division ATP-binding protein FtsE -
  FYA20_RS10860 (FYA20_10860) pilA 2237766..2239262 (-) 1497 WP_001040641.1 signal recognition particle-docking protein FtsY Machinery gene
  FYA20_RS10865 (FYA20_10865) rsmD 2239412..2240008 (+) 597 WP_000743193.1 16S rRNA (guanine(966)-N(2))-methyltransferase -
  FYA20_RS10870 (FYA20_10870) yhhL 2239998..2240267 (+) 270 WP_001295207.1 DUF1145 family protein -
  FYA20_RS10875 (FYA20_10875) yhhM 2240270..2240629 (-) 360 WP_000042904.1 DUF2500 domain-containing protein -
  FYA20_RS10880 (FYA20_10880) yhhN 2240770..2241396 (+) 627 WP_000964718.1 lysoplasmalogenase -
  FYA20_RS10885 (FYA20_10885) zntA 2241470..2243668 (+) 2199 WP_000106604.1 Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase ZntA -
  FYA20_RS10890 (FYA20_10890) tusA 2243937..2244182 (-) 246 WP_000130621.1 sulfurtransferase TusA -

Sequence

Protein


Download         Length: 498 a.a.        Molecular weight: 54614.37 Da        Isoelectric Point: 4.1733

>NTDB_id=334420 FYA20_RS10860 WP_001040641.1 2237766..2239262(-) (pilA) [Escherichia coli O2:H6 strain PG20180057]
MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEPVKASEHAVEEQPQAHTEAEAETFAADVVEVTEQVAE
SEKAQPEAEVVAQPEPVVEETPEPVAIEREELPLSEDVNAEAVSPEEWQAEAETVEIVEAAEEEAAKEEITDEEPEAQAL
AAEAAEEAVMVVSPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISLFRGKKIDDDLFEELEEQLLIAD
VGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEMGEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQ
QGKSVMLAAGDTFRAAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKSHLMEELKK
IVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLDGTAKGGVIFSVADQFGIPIRYIGVGERIED
LRPFKADDFIEALFARED

Nucleotide


Download         Length: 1497 bp        

>NTDB_id=334420 FYA20_RS10860 WP_001040641.1 2237766..2239262(-) (pilA) [Escherichia coli O2:H6 strain PG20180057]
ATGGCGAAAGAAAAAAAACGTGGCTTTTTTTCCTGGCTGGGCTTTGGTCAAAAAGAGCAGACCCCGGAAAAAGAGACAGA
AGTTCAGAATGAACAACCAGTTGTAGAAGAAATCGTTCAGGCGCAAGAGCCTGTGAAGGCTTCTGAACACGCCGTTGAAG
AGCAGCCGCAGGCGCATACTGAAGCCGAGGCGGAAACTTTTGCTGCCGACGTTGTGGAAGTTACTGAACAGGTTGCTGAA
AGTGAAAAAGCGCAGCCTGAAGCGGAAGTCGTTGCACAGCCGGAACCGGTCGTAGAAGAAACGCCGGAGCCAGTGGCTAT
CGAACGTGAAGAACTGCCGTTGTCGGAAGACGTCAACGCCGAAGCGGTTTCGCCAGAAGAGTGGCAGGCCGAAGCGGAAA
CCGTAGAGATTGTCGAAGCGGCGGAAGAAGAAGCGGCTAAAGAAGAAATCACCGACGAAGAGCCGGAGGCACAGGCGCTG
GCTGCCGAAGCGGCAGAAGAGGCGGTAATGGTGGTTTCTCCGGCAGAAGAAGAGCAGCCGGTGGAAGAAATCGCTCAGGA
GCAGGAAAAACCGACCAAAGAAGGTTTCTTCGCGCGCCTGAAACGCAGCCTGTTAAAAACCAAAGAAAATCTCGGTTCCG
GATTTATCAGCCTGTTCCGCGGTAAAAAAATCGACGATGATCTGTTTGAAGAGCTGGAAGAACAGCTGTTGATCGCCGAT
GTGGGCGTGGAAACCACTCGTAAAATTATCACCAATCTGACGGAAGGCGCATCCCGCAAGCAGCTTCGTGATGCCGAGGC
GCTCTATGGCCTGCTGAAAGAAGAGATGGGCGAGATTCTGGCGAAAGTCGATGAGCCGCTGAATGTTGAAGGTAAAACGC
CGTTCGTGATCCTGATGGTGGGCGTCAACGGTGTGGGTAAAACCACGACGATTGGTAAGCTGGCGCGTCAGTTTGAGCAG
CAGGGTAAATCGGTGATGCTGGCGGCAGGCGATACTTTCCGTGCAGCAGCGGTTGAACAGCTTCAGGTCTGGGGTCAGCG
CAACAATATTCCGGTGATTGCCCAGCATACCGGTGCGGATTCCGCTTCTGTTATCTTCGATGCCATTCAGGCCGCTAAAG
CGCGTAACATCGACGTCCTGATTGCCGATACCGCCGGACGCCTGCAAAACAAATCGCACCTGATGGAAGAGTTGAAGAAA
ATCGTCCGCGTGATGAAGAAACTCGACGTTGAAGCTCCGCATGAAGTTATGCTGACCATCGATGCCAGCACCGGGCAGAA
TGCGGTAAGCCAGGCCAAACTGTTCCACGAAGCCGTTGGCTTAACCGGCATCACGCTAACGAAACTGGACGGCACGGCGA
AAGGCGGGGTAATTTTCTCGGTGGCTGACCAGTTTGGTATCCCTATCCGCTACATTGGTGTCGGCGAACGTATTGAGGAT
TTGCGTCCGTTTAAGGCGGACGACTTTATAGAGGCACTTTTTGCCCGAGAGGATTAA

Domains

Predicted by InterProScan.

SRP54-type protein, GTPase domain (SRP54)

(294-494)

SRP54-type protein, helical bundle domain (SRP54_N)

(211-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

48.831

77.309

0.378