Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   EOV66_RS20035 Genome accession   NZ_CP043033
Coordinates   4096722..4097162 (+) Length   146 a.a.
NCBI ID   WP_000360914.1    Uniprot ID   A0A0H2YVZ3
Organism   Escherichia coli strain XDL     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4091722..4102162
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EOV66_RS20015 (EOV66_20025) aroP 4092720..4094093 (+) 1374 WP_000969915.1 aromatic amino acid transporter AroP -
  EOV66_RS20020 (EOV66_20030) ampE 4094136..4094990 (-) 855 WP_000172020.1 beta-lactamase regulator AmpE -
  EOV66_RS20025 (EOV66_20035) ampD 4094987..4095538 (-) 552 WP_000923703.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  EOV66_RS20030 (EOV66_20040) nadC 4095626..4096519 (+) 894 WP_001135167.1 carboxylating nicotinate-nucleotide diphosphorylase -
  EOV66_RS20035 (EOV66_20045) pilA 4096722..4097162 (+) 441 WP_000360914.1 prepilin peptidase-dependent pilin Machinery gene
  EOV66_RS20040 (EOV66_20050) pilB 4097172..4098557 (+) 1386 WP_001025211.1 type II secretion system protein GspE Machinery gene
  EOV66_RS20045 (EOV66_20055) hofC 4098547..4099749 (+) 1203 WP_000157219.1 protein transport protein HofC -
  EOV66_RS20050 (EOV66_20060) guaC 4099784..4100827 (-) 1044 WP_001217333.1 GMP reductase -
  EOV66_RS27505 - 4100983..4101027 (-) 45 WP_120795372.1 protein YacM -
  EOV66_RS20055 (EOV66_20065) coaE 4101052..4101672 (+) 621 WP_001269510.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15620.77 Da        Isoelectric Point: 4.5237

>NTDB_id=334288 EOV66_RS20035 WP_000360914.1 4096722..4097162(+) (pilA) [Escherichia coli strain XDL]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLNTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=334288 EOV66_RS20035 WP_000360914.1 4096722..4097162(+) (pilA) [Escherichia coli strain XDL]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAAATACCTGCGACGGTGGCAGCAATGGTATTCCTTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACGCCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2YVZ3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363