Detailed information NTG0033416

Bioinformatically predicted

Overview

• Name: comGF/cglF
• Type: Machinery gene
• Locus tag: SPT_RS10115
• Genome accession: NC_012469
• Coordinates: 1903685..1904146 (-)
• Length: 153 a.a.
• NCBI ID: WP_000250545.1
• Uniprot ID: -
• Organism: Streptococcus pneumoniae Taiwan19F-14
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1898685..1909146

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
SPT_RS10085 (SPT_2036)	-	1898996..1899820 (-)	825	WP_000727904.1	membrane protein insertase YidC	-
SPT_RS10090 (SPT_2037)	rnpA	1899795..1900166 (-)	372	WP_000739246.1	ribonuclease P protein component	-
SPT_RS13270 (SPT_2038)	-	1900183..1900314 (-)	132	WP_000768904.1	hypothetical protein	-
SPT_RS10095 (SPT_2039)	-	1900315..1901505 (-)	1191	WP_000167766.1	acetate kinase	-
SPT_RS10100 (SPT_2040)	comYH	1901556..1902509 (-)	954	WP_000345119.1	class I SAM-dependent methyltransferase	Machinery gene
SPT_RS10105 (SPT_2041)	-	1902570..1903157 (-)	588	WP_000679774.1	class I SAM-dependent methyltransferase	-
SPT_RS10110 (SPT_2042)	comGG/cglG	1903294..1903707 (-)	414	WP_000265630.1	competence type IV pilus minor pilin ComGG	Machinery gene
SPT_RS10115 (SPT_2043)	comGF/cglF	1903685..1904146 (-)	462	WP_000250545.1	competence type IV pilus minor pilin ComGF	Machinery gene
SPT_RS10120 (SPT_2044)	comGE/cglE	1904109..1904411 (-)	303	WP_000413380.1	competence type IV pilus minor pilin ComGE	Machinery gene
SPT_RS10125 (SPT_2045)	comGD/cglD	1904374..1904778 (-)	405	WP_000588005.1	competence type IV pilus minor pilin ComGD	Machinery gene
SPT_RS10130 (SPT_2046)	comGC/cglC	1904771..1905097 (-)	327	WP_000738650.1	comG operon protein ComGC	Machinery gene
SPT_RS10135 (SPT_2047)	comGB/cglB	1905099..1906115 (-)	1017	WP_013193332.1	competence type IV pilus assembly protein ComGB	Machinery gene
SPT_RS10140 (SPT_2048)	comGA/cglA/cilD	1906063..1907004 (-)	942	WP_000249567.1	competence type IV pilus ATPase ComGA	Machinery gene
SPT_RS10145 (SPT_2049)	-	1907080..1907445 (-)	366	WP_000286415.1	DUF1033 family protein	-
SPT_RS10150 (SPT_2050)	-	1907596..1908654 (-)	1059	WP_000649468.1	zinc-dependent alcohol dehydrogenase family protein	-

Sequence

Protein

• Length: 153 a.a.
• Molecular weight: 17855.34 Da
• Isoelectric Point: 6.4832

>NTDB_id=33416 SPT_RS10115 WP_000250545.1 1903685..1904146(-) (comGF/cglF) [Streptococcus pneumoniae Taiwan19F-14]
MVQNSCWQSKSHKVKAFTLLESLLALLVISGGLLLFQAMSQLLISEVRYQQQSEQKEWLLFVDQLEAELDRSQFEKVEGN
RFYMKQDGKDIAIGKSKSDDFRKTDASGRGYQPMVYGLKSAQITEDNQLVHFRFQFQKGLEREFIYRVEKEES

Nucleotide

• Length: 462 bp

>NTDB_id=33416 SPT_RS10115 WP_000250545.1 1903685..1904146(-) (comGF/cglF) [Streptococcus pneumoniae Taiwan19F-14]
ATGGTTCAGAACAGTTGTTGGCAATCAAAGAGCCATAAGGTCAAGGCTTTTACCTTGTTAGAATCCCTGCTTGCCCTGCT
CGTCATCAGTGGAGGCTTACTCCTTTTTCAAGCTATGAGTCAGCTCCTCATTTCAGAAGTTCGCTACCAGCAACAAAGCG
AGCAAAAGGAGTGGCTCTTGTTTGTGGATCAACTTGAGGCAGAATTAGACCGTTCGCAGTTCGAAAAAGTGGAAGGCAAT
CGTTTCTATATGAAGCAAGATGGCAAGGACATCGCCATCGGTAAGTCAAAGTCAGACGATTTTCGTAAAACCGATGCTAG
TGGACGGGGGTATCAGCCTATGGTTTATGGCCTCAAATCAGCTCAGATTACAGAGGACAATCAACTGGTTCATTTTCGTT
TCCAGTTTCAAAAAGGCTTAGAAAGGGAGTTCATCTATCGTGTGGAAAAAGAAGAAAGTTAA

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (45-141)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF/cglF	Streptococcus pneumoniae Rx1	93.464	100	0.935
comGF/cglF	Streptococcus pneumoniae D39	93.464	100	0.935
comGF/cglF	Streptococcus pneumoniae R6	93.464	100	0.935
comGF/cglF	Streptococcus pneumoniae TIGR4	93.464	100	0.935
comGF/cglF	Streptococcus mitis SK321	92.81	100	0.928
comGF/cglF	Streptococcus mitis NCTC 12261	91.503	100	0.915