Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   EPK90_RS19310 Genome accession   NZ_CP035034
Coordinates   4054857..4056239 (-) Length   460 a.a.
NCBI ID   WP_013024514.1    Uniprot ID   D4GJH6
Organism   Pantoea ananatis strain NN08200     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 4049857..4061239
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EPK90_RS19285 (EPK90_19285) - 4050293..4050982 (-) 690 WP_014606640.1 molecular chaperone -
  EPK90_RS19290 (EPK90_19290) - 4051022..4051576 (-) 555 WP_014606641.1 fimbrial protein -
  EPK90_RS19295 (EPK90_19295) - 4051819..4052268 (-) 450 WP_022622279.1 hypothetical protein -
  EPK90_RS19300 (EPK90_19300) - 4052265..4053197 (-) 933 WP_019106381.1 winged helix-turn-helix domain-containing protein -
  EPK90_RS19305 (EPK90_19305) nadR 4053526..4054764 (-) 1239 WP_014606644.1 multifunctional transcriptional regulator/nicotinamide-nucleotide adenylyltransferase/ribosylnicotinamide kinase NadR -
  EPK90_RS19310 (EPK90_19310) radA 4054857..4056239 (-) 1383 WP_013024514.1 DNA repair protein RadA Machinery gene
  EPK90_RS19315 (EPK90_19315) serB 4056253..4057230 (-) 978 WP_014595282.1 phosphoserine phosphatase -
  EPK90_RS19320 (EPK90_19320) - 4057338..4058006 (+) 669 WP_089517614.1 YtjB family periplasmic protein -
  EPK90_RS19325 (EPK90_19325) yjjG 4058135..4058815 (-) 681 WP_014595280.1 pyrimidine 5'-nucleotidase -
  EPK90_RS19330 (EPK90_19330) rimI 4058835..4059275 (-) 441 WP_014606647.1 ribosomal protein S18-alanine N-acetyltransferase -
  EPK90_RS19335 (EPK90_19335) - 4059247..4059657 (-) 411 WP_013024509.1 DNA polymerase III subunit psi -
  EPK90_RS19340 (EPK90_19340) rsmC 4059763..4060794 (+) 1032 WP_029570981.1 16S rRNA (guanine(1207)-N(2))-methyltransferase RsmC -

Sequence

Protein


Download         Length: 460 a.a.        Molecular weight: 49450.09 Da        Isoelectric Point: 7.6663

>NTDB_id=334067 EPK90_RS19310 WP_013024514.1 4054857..4056239(-) (radA) [Pantoea ananatis strain NN08200]
MAKAAKRAFVCNECGADYPRWQGQCSACHAWNTITEVRIAASPAAARNERLSGYAGSAGTSRVQKLSEISLEALPRFSTS
FKEFDRVLGGGVVPGSAILIGGNPGAGKSTLLLQVMCRLAQNMKTLYVTGEESLQQVAMRAHRLGLPTENLNMLSETSIE
QICLIAEQEAPQIMVIDSIQVMHMADIQSSPGSVAQVRETAAYLTRFAKTRGVAIIMVGHVTKDGSLAGPKVLEHCIDCS
VLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQGMREVSNPSAIFLSRGEEITSGSSVMVLWEGTRPLLVEIQALVDH
SMMGNPRRVAVGLEQNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALMLSMVSSLRDRPLPQDLVIFGEV
GLAGEIRPVPSGQERISEAAKHGFKRAIVPAGNAPKKPIEGMRVYSAKKLADALAILDDL

Nucleotide


Download         Length: 1383 bp        

>NTDB_id=334067 EPK90_RS19310 WP_013024514.1 4054857..4056239(-) (radA) [Pantoea ananatis strain NN08200]
TTGGCCAAAGCGGCAAAACGCGCATTTGTTTGTAACGAGTGTGGCGCAGATTATCCACGCTGGCAGGGGCAATGCAGCGC
CTGTCACGCCTGGAACACCATTACCGAGGTACGGATTGCCGCCTCACCCGCTGCAGCGCGCAATGAGCGCCTCAGCGGTT
ATGCGGGCAGTGCCGGGACCAGTCGGGTGCAAAAGCTGTCCGAAATCAGCCTGGAAGCCTTGCCCCGTTTCTCGACCAGC
TTTAAAGAATTTGACCGTGTGCTGGGCGGTGGCGTGGTGCCCGGCAGTGCCATTCTGATTGGTGGTAATCCCGGGGCCGG
TAAATCCACTTTGTTGCTGCAGGTCATGTGCCGCTTAGCGCAGAACATGAAAACGCTGTATGTCACCGGCGAAGAATCTT
TACAGCAGGTGGCAATGCGTGCCCATCGGCTGGGTTTGCCGACTGAAAACCTGAACATGCTGTCGGAAACCAGCATCGAA
CAGATCTGCCTGATCGCCGAGCAGGAAGCGCCGCAAATCATGGTCATCGACTCCATCCAGGTGATGCACATGGCCGATAT
CCAGTCTTCACCCGGCAGCGTCGCCCAGGTGCGCGAAACCGCCGCTTATCTGACGCGCTTTGCCAAAACACGCGGCGTGG
CGATTATCATGGTGGGCCATGTCACCAAAGACGGATCGCTGGCCGGGCCGAAGGTCCTTGAGCACTGTATCGACTGTTCG
GTGCTGCTGGATGGCGATGCCGATTCGCGCTTCCGCACCTTGCGCAGCCATAAAAACCGTTTTGGTGCCGTGAATGAACT
GGGGGTTTTTGCCATGACGGAGCAGGGCATGCGTGAGGTGAGCAATCCTTCCGCCATTTTCCTGTCGCGTGGTGAAGAGA
TTACGTCGGGCAGTTCGGTCATGGTGCTGTGGGAAGGAACCCGGCCATTGCTGGTCGAGATTCAGGCGCTGGTGGACCAT
TCGATGATGGGGAATCCCCGTCGTGTTGCCGTCGGGCTTGAACAAAACCGCCTGGCGATTCTGCTGGCGGTACTGCATCG
TCACGGTGGATTGCAAATGGCGGATCAGGATGTGTTCGTCAACGTCGTGGGCGGCGTGAAAGTCACAGAAACCAGCGCCG
ACCTGGCGCTGATGCTGTCAATGGTTTCCAGCCTGCGCGATCGCCCCTTGCCGCAGGATCTGGTTATCTTTGGCGAAGTC
GGGCTGGCCGGCGAAATTCGCCCGGTGCCCAGCGGCCAGGAACGGATTTCAGAAGCGGCTAAACATGGCTTCAAACGGGC
GATTGTGCCTGCCGGTAACGCGCCGAAAAAGCCGATTGAGGGCATGCGCGTCTACAGCGCGAAAAAGTTGGCGGATGCGC
TGGCGATTCTCGATGATCTTTAA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-36)

AAA domain (AAA_25)

(87-225)

Subunit ChlI of Mg-chelatase (ChlI)

(347-433)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB D4GJH6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

48.59

100

0.487
  radA Streptococcus mitis NCTC 12261

43.516

98.913

0.43
  radA Streptococcus pneumoniae Rx1

44.78

93.696

0.42
  radA Streptococcus pneumoniae D39

44.78

93.696

0.42
  radA Streptococcus pneumoniae R6

44.78

93.696

0.42
  radA Streptococcus pneumoniae TIGR4

44.78

93.696

0.42
  radA Streptococcus mitis SK321

44.78

93.696

0.42

Multiple sequence alignment