Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   EOK76_RS02940 Genome accession   NZ_CP034973
Coordinates   613426..614790 (+) Length   454 a.a.
NCBI ID   WP_003580798.1    Uniprot ID   -
Organism   Lacticaseibacillus paracasei strain ALAC-4     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 608426..619790
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EOK76_RS02915 (EOK76_g0617) - 608720..610066 (-) 1347 WP_003576225.1 aminopeptidase C -
  EOK76_RS02920 (EOK76_g0618) - 610299..611618 (-) 1320 WP_003571381.1 C1 family peptidase -
  EOK76_RS02925 (EOK76_g0619) - 611863..612240 (+) 378 WP_003567260.1 DUF805 domain-containing protein -
  EOK76_RS02930 (EOK76_g0620) - 612355..612645 (-) 291 WP_003567258.1 GNAT family N-acetyltransferase -
  EOK76_RS02935 (EOK76_g0621) - 612867..613409 (+) 543 WP_003567256.1 dUTP diphosphatase -
  EOK76_RS02940 (EOK76_g0622) radA 613426..614790 (+) 1365 WP_003580798.1 DNA repair protein RadA Machinery gene
  EOK76_RS02945 (EOK76_g0623) - 614812..615927 (+) 1116 WP_003567252.1 PIN/TRAM domain-containing protein -
  EOK76_RS02950 (EOK76_g0624) - 616031..616927 (-) 897 WP_003580795.1 helix-turn-helix domain-containing protein -
  EOK76_RS02955 (EOK76_g0625) - 617056..617187 (+) 132 WP_003580794.1 hypothetical protein -
  EOK76_RS02960 - 617208..618113 (+) 906 WP_016366635.1 ATP-binding cassette domain-containing protein -
  EOK76_RS02965 (EOK76_g0627) - 618117..618893 (+) 777 WP_003577837.1 ABC transporter permease -
  EOK76_RS02970 - 618937..619041 (+) 105 WP_191994146.1 bacteriocin -
  EOK76_RS02975 (EOK76_g0628) - 619085..619198 (+) 114 WP_003577835.1 bacteriocin -

Sequence

Protein


Download         Length: 454 a.a.        Molecular weight: 49140.50 Da        Isoelectric Point: 7.4216

>NTDB_id=333811 EOK76_RS02940 WP_003580798.1 613426..614790(+) (radA) [Lacticaseibacillus paracasei strain ALAC-4]
MAKAKTQYVCQNCGYISATYLGRCPNCGGWNTLVEETVSSSKSVPRQTAAGSKIKPTRMNDVTITKETRVKTGLDELNRV
LGGGVVPGSLVLIGGDPGIGKSTLLLQVSGQLANTGGTVLYVSGEESASQIKMRAGRLGVANSGMYLYPETDMPSIEDVI
NQMQPDYVVIDSVQTMNVPEMNSAVGSVAQIREVTAELMRIAKSKGVTIFIVGHVTKEGAIAGPKILEHMVDTVLYFEGD
MHHTYRILRSVKNRFGSTNEIGIFEMHQDGLQEVANPSEIFLEERLAGATGSAVVVSMEGTRPILVEIQALISPTMYGNA
KRTSSGLDHNRVSLIMAVLEKRANLMLQNQDAYLKATGGVKLDEPAIDLAMAVAIASSYRDKEISPTDCFVGEIGLTGEV
RRVNRIEERVKEAAKLGFKRIFVPRNNLQGWHAPKDIQVIGVTSIAEALHKVFN

Nucleotide


Download         Length: 1365 bp        

>NTDB_id=333811 EOK76_RS02940 WP_003580798.1 613426..614790(+) (radA) [Lacticaseibacillus paracasei strain ALAC-4]
ATGGCGAAAGCAAAAACACAATATGTTTGCCAAAACTGTGGCTATATTTCAGCCACTTATCTAGGCCGGTGTCCAAATTG
CGGCGGCTGGAACACCTTAGTGGAAGAAACCGTCAGTTCAAGCAAGTCTGTGCCGCGACAAACCGCAGCCGGAAGCAAGA
TAAAGCCGACGCGGATGAACGATGTGACGATCACCAAAGAAACGCGCGTCAAGACTGGCTTAGATGAACTGAACCGGGTG
CTTGGCGGCGGAGTGGTGCCAGGGTCACTGGTGCTGATCGGCGGGGATCCGGGGATTGGTAAATCAACCTTGCTATTACA
AGTATCAGGGCAGTTGGCTAATACCGGCGGAACGGTTTTGTATGTCTCTGGCGAAGAAAGTGCCAGTCAGATCAAAATGC
GGGCTGGCCGGCTTGGCGTGGCAAATTCGGGGATGTACTTGTATCCCGAAACCGACATGCCGAGTATTGAAGACGTCATC
AACCAAATGCAGCCGGATTATGTTGTGATCGACTCCGTTCAAACTATGAATGTGCCAGAAATGAATTCAGCGGTCGGTTC
TGTGGCACAGATTCGCGAAGTGACGGCAGAACTGATGCGCATTGCCAAGTCCAAAGGCGTGACCATTTTCATCGTTGGCC
ATGTCACTAAGGAAGGGGCGATTGCCGGGCCGAAGATTCTTGAGCATATGGTCGACACGGTGCTCTATTTTGAAGGCGAC
ATGCACCACACGTATCGAATCTTAAGATCCGTCAAAAATCGATTTGGATCGACCAATGAAATCGGCATTTTTGAAATGCA
TCAAGACGGGTTACAGGAAGTCGCCAATCCCTCGGAAATCTTCTTGGAAGAACGCTTGGCGGGGGCAACCGGTTCTGCGG
TGGTTGTTTCGATGGAAGGCACCCGACCAATCTTAGTCGAGATTCAAGCCTTGATCAGTCCAACGATGTATGGCAATGCT
AAACGGACGAGCAGTGGGTTGGATCACAATCGAGTGAGCTTGATCATGGCCGTTCTTGAGAAGCGGGCTAACTTGATGTT
GCAAAATCAGGACGCCTATCTCAAGGCAACAGGCGGTGTGAAACTCGATGAGCCGGCGATTGACCTCGCCATGGCTGTGG
CAATTGCGTCATCTTATCGAGATAAGGAGATTTCACCGACAGATTGTTTTGTCGGTGAAATCGGATTGACGGGGGAAGTG
CGCCGCGTCAATCGGATCGAAGAACGTGTCAAAGAGGCGGCCAAACTGGGTTTCAAACGGATTTTTGTACCCCGTAACAA
CTTGCAAGGCTGGCATGCGCCAAAAGATATTCAAGTCATTGGTGTTACAAGCATTGCCGAAGCGCTGCATAAGGTCTTTA
ATTAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(69-219)

Subunit ChlI of Mg-chelatase (ChlI)

(363-427)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis NCTC 12261

68.212

99.78

0.681
  radA Streptococcus pneumoniae Rx1

68.212

99.78

0.681
  radA Streptococcus pneumoniae D39

68.212

99.78

0.681
  radA Streptococcus pneumoniae R6

68.212

99.78

0.681
  radA Streptococcus pneumoniae TIGR4

68.212

99.78

0.681
  radA Streptococcus mitis SK321

67.991

99.78

0.678
  radA Bacillus subtilis subsp. subtilis str. 168

64.537

100

0.645

Multiple sequence alignment