Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   EO082_RS02000 Genome accession   NZ_CP034967
Coordinates   416868..418307 (+) Length   479 a.a.
NCBI ID   WP_005336331.1    Uniprot ID   A0A0T6N7F3
Organism   Aeromonas veronii strain ZfB1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 411868..423307
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EO082_RS01970 (EO082_01980) mlaF 412445..413245 (-) 801 WP_005336343.1 phospholipid ABC transporter ATP-binding protein MlaF -
  EO082_RS01975 (EO082_01985) - 413453..414448 (+) 996 WP_005356672.1 KpsF/GutQ family sugar-phosphate isomerase -
  EO082_RS01980 (EO082_01990) kdsC 414448..415002 (+) 555 WP_164535919.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  EO082_RS01985 (EO082_01995) lptC 414999..415559 (+) 561 WP_005336338.1 LPS export ABC transporter periplasmic protein LptC -
  EO082_RS01990 (EO082_02000) lptA 415546..416073 (+) 528 WP_040068817.1 lipopolysaccharide transport periplasmic protein LptA -
  EO082_RS01995 (EO082_02005) lptB 416077..416802 (+) 726 WP_005336333.1 LPS export ABC transporter ATP-binding protein -
  EO082_RS02000 (EO082_02010) rpoN/rpoN1 416868..418307 (+) 1440 WP_005336331.1 RNA polymerase factor sigma-54 Machinery gene
  EO082_RS02005 (EO082_02015) hpf 418328..418615 (+) 288 WP_005336323.1 ribosome hibernation promoting factor -
  EO082_RS02010 (EO082_02020) ptsN 418618..419064 (+) 447 WP_005336322.1 PTS IIA-like nitrogen regulatory protein PtsN -
  EO082_RS02015 (EO082_02025) rapZ 419110..419976 (+) 867 WP_021231839.1 RNase adapter RapZ -
  EO082_RS02020 (EO082_02030) npr 419997..420269 (+) 273 WP_005336318.1 PTS phosphocarrier protein NPr -
  EO082_RS02025 (EO082_02035) degS 420461..421564 (-) 1104 WP_164535920.1 outer membrane-stress sensor serine endopeptidase DegS -
  EO082_RS02030 (EO082_02040) - 421674..423035 (-) 1362 WP_164535921.1 Do family serine endopeptidase -

Sequence

Protein


Download         Length: 479 a.a.        Molecular weight: 53701.64 Da        Isoelectric Point: 4.3442

>NTDB_id=333757 EO082_RS02000 WP_005336331.1 416868..418307(+) (rpoN/rpoN1) [Aeromonas veronii strain ZfB1]
MKPTLQLRLGQSLTMTPQLQQAIRLLQLSTLELQQEIQQALENNPLLEQEESEAQETSPEAPVDASQLDSSDAMAQEQMP
DELPVDTTWDEVYSAGTAQSAGPIHDGEDSVYQGETTENLQDYLLWQMRLTPFSDVDAAIALAIIDAIDESGYLTVALED
IQAAVTSDDVEVELDEVEAVLKRVQHFDPVGIAARSVQECLLIQLDQYDPQTPWLNEAKEVILEHMELLGNRDYRTLARK
TKLKESDLKEVLDLIQLLEPRPGNGIIQSDSQYVIPDVSVVKKGSKWVVELNPDSAPRIRVNETYAAMARNARSSTDTQF
IRSHLQEAKWFIKSLESRNDTLLKVASCIVEQQQGFFEYGEEAMKPMVLNDIAEAVEMHESTISRVTTQKYMHTPRGIFE
LKFFFSSHVNTEGGGECSSTAIRALIKKLVTAENPAKPLSDSKIADLLAEQGIMVARRTIAKYRESLFIPPSNQRKRLV

Nucleotide


Download         Length: 1440 bp        

>NTDB_id=333757 EO082_RS02000 WP_005336331.1 416868..418307(+) (rpoN/rpoN1) [Aeromonas veronii strain ZfB1]
ATGAAGCCGACATTACAGTTGCGTCTCGGTCAGTCCCTGACCATGACGCCGCAATTACAACAAGCTATTCGTCTGCTCCA
GCTCTCCACTCTGGAACTTCAGCAGGAAATTCAGCAAGCGCTCGAAAATAACCCTCTGCTGGAGCAGGAAGAGAGCGAGG
CGCAAGAGACCAGTCCGGAAGCGCCGGTCGATGCCAGTCAGCTGGATTCCAGCGATGCCATGGCTCAGGAGCAGATGCCG
GACGAGTTACCGGTGGATACCACCTGGGACGAGGTTTACTCTGCCGGCACTGCCCAAAGTGCGGGCCCCATCCACGATGG
GGAAGATAGCGTCTATCAGGGTGAAACTACCGAAAACCTGCAGGATTATCTGCTGTGGCAGATGCGGCTCACACCGTTCA
GCGATGTGGATGCCGCCATCGCGCTCGCCATCATCGATGCCATCGACGAATCCGGTTATCTGACCGTTGCGCTGGAGGAT
ATTCAGGCTGCCGTCACCAGCGATGACGTAGAGGTGGAACTCGATGAGGTCGAGGCGGTACTCAAGCGGGTGCAGCACTT
CGATCCGGTCGGCATTGCTGCCCGTTCTGTGCAGGAGTGTTTGCTGATCCAGCTGGATCAGTATGACCCGCAGACCCCCT
GGCTCAACGAGGCCAAGGAGGTGATCCTCGAGCACATGGAGCTGCTCGGCAACCGGGATTACCGCACCCTCGCCCGCAAG
ACAAAACTCAAAGAGAGCGATCTCAAGGAGGTGCTGGATCTGATCCAGCTACTGGAGCCGCGCCCCGGCAACGGCATCAT
CCAGAGCGACTCCCAGTATGTGATCCCCGACGTCTCCGTGGTCAAGAAGGGCAGCAAGTGGGTGGTCGAGCTCAATCCCG
ACTCAGCCCCCCGCATCCGGGTCAACGAGACCTATGCCGCCATGGCCCGCAACGCCCGCAGCAGCACCGACACCCAGTTT
ATCCGCTCCCATTTGCAGGAAGCCAAGTGGTTCATCAAGAGCCTGGAAAGTCGCAATGACACCCTGCTGAAAGTGGCCAG
TTGCATTGTCGAGCAGCAACAGGGCTTTTTCGAGTATGGTGAAGAGGCGATGAAGCCGATGGTGCTCAATGACATCGCCG
AAGCGGTAGAGATGCATGAGTCGACCATCTCCCGGGTGACGACCCAGAAGTACATGCATACCCCCCGTGGTATCTTCGAA
TTGAAGTTTTTTTTCTCCAGCCATGTGAACACCGAAGGAGGAGGAGAGTGCTCATCGACCGCAATTCGCGCGCTCATCAA
GAAGCTGGTCACGGCAGAGAATCCTGCCAAGCCGTTAAGTGATAGCAAGATCGCGGATTTGCTGGCCGAACAGGGTATTA
TGGTGGCGAGACGCACGATCGCTAAGTATCGCGAATCCTTGTTTATCCCACCTTCCAATCAGCGCAAACGCCTGGTTTAG

Domains

Predicted by InterProScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-305)

Sigma-54, DNA binding domain (Sigma54_DBD)

(319-477)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.988

100

0.491

Multiple sequence alignment