Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   EO946_RS05735 Genome accession   NZ_CP034943
Coordinates   1109906..1113406 (+) Length   1166 a.a.
NCBI ID   WP_003224417.1    Uniprot ID   -
Organism   Bacillus spizizenii ATCC 6633 = JCM 2499 strain ATCC 6633     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1104906..1118406
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EO946_RS05715 (EO946_05715) - 1105760..1106962 (-) 1203 WP_003224407.1 MFS transporter -
  EO946_RS05720 (EO946_05720) - 1107076..1108803 (+) 1728 WP_003224409.1 SgrR family transcriptional regulator -
  EO946_RS05725 (EO946_05725) - 1108839..1109168 (-) 330 WP_032727222.1 four-helix bundle copper-binding protein -
  EO946_RS05730 (EO946_05730) - 1109285..1109722 (-) 438 WP_003224414.1 ferritin family protein -
  EO946_RS05735 (EO946_05735) addB 1109906..1113406 (+) 3501 WP_003224417.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  EO946_RS05740 (EO946_05740) addA 1113393..1117097 (+) 3705 WP_003224421.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  EO946_RS05745 (EO946_05745) sbcD 1117169..1118344 (+) 1176 WP_003224424.1 exonuclease subunit SbcD -

Sequence

Protein


Download         Length: 1166 a.a.        Molecular weight: 134785.85 Da        Isoelectric Point: 5.5249

>NTDB_id=333562 EO946_RS05735 WP_003224417.1 1109906..1113406(+) (addB) [Bacillus spizizenii ATCC 6633 = JCM 2499 strain ATCC 6633]
MGAEFLVGRSGSGKTKLIIDSIQDELRRAPFGKPIIFLVPDQMTFLMEYELAKTPDMGGMIRAQVFSFSRLAWRVLQHTG
GMSRPFLTSTGVQMLLRKLIEEHKQEFKVYQKASDKSGFTAQVERMLTEFKRYCLEPEDIRRMAESGTASEYRGERVLSE
KLHDLSILYQQMEKSLADQYLHSEDYLTLLAEHIPLAEDIKGAHIYVDGFYQFTPQEFRVLEQLMVHAEHITFSLTADKP
SYEREPHELELFRMTGKTYYRLHQKAKELNLDITYKELSGIERHTQTPELAHLEAQYEARPAIPYAEKQEAFTVMQAANR
RAELEGIAREIHTLVREKGYRYKDVAILARQPEDYKDMVKEVFADYEIPYFIDGKASMLNHPLIEFIRSSIDVLKGNWRY
EAVFRCVKTELLFPLNEPKTKVREQVDQLENYCIAYGIKGDRWTKGDRFHYRRFVSLDDDFAQTDQEIEMENMLNDTRDW
IVPPLFQFQKRMKKAKTVQEKAEALYRYLEETDVPLKLDQERQRAEEDGRIIEAQQHQQAWDAVIQLLEEFVEMMGDDEI
SLDLFQQMMEAGAESLTFSLIPPALDQVFVGNMDLSRMYGTSCTFVLGANDGVLPARPDENGVLSDDDREWLKTIGIELS
SGGRERLLDEHFLIYMAFSSPSDRLYVSYPIADAEGKTLLPSIVIKRLEELFPQHKERLLTNEPEQVSDEEQLMYVVNKS
VAQSFTASQLRLWTREYDISDVWWSTYNVLMSEPDRLQSKKLFSSLFFRNEVKQLERSVSRQLYGERIQGSVSRMETFNA
CPFSHFASHGLHLKERQFFKLEAPDIGQLFHSSLKLISDRLREQKMNWRDLTKEQCELFSYDAVERLAPKLQKEILLSSN
RHYYVKEKLQKIVTRVSGILSEHAKASGFVPIGLELGFGGKGPLPPLTFKLKNGCTMELVGRIDRVDKAESSKGLLLRIV
DYKSSDKGLDLAEVYYGLALQMLTYLDLSITHSADWLGMRATPAGVLYFHIHDPMIQSNLPLGLDEIEQEIFKKFKMKGL
LLGDQEAVRLMDTTLQEGRSNIINAGLKKDGSLRSDSAAVGEKEFELLTKHVRRTFQEAGEQITDGRVSIEPYKMKNKTP
CTYCAFKSVCQFDESLEENEYRPLKAEKDKTILEWIKKEADGNEHS

Nucleotide


Download         Length: 3501 bp        

>NTDB_id=333562 EO946_RS05735 WP_003224417.1 1109906..1113406(+) (addB) [Bacillus spizizenii ATCC 6633 = JCM 2499 strain ATCC 6633]
TTGGGAGCAGAGTTTTTAGTAGGCAGGTCCGGGAGTGGAAAAACGAAGCTGATCATCGACAGCATTCAGGATGAACTGCG
CCGGGCTCCATTCGGGAAACCGATCATTTTTCTAGTCCCGGATCAAATGACGTTTTTAATGGAATACGAGCTTGCTAAAA
CGCCTGATATGGGCGGAATGATACGTGCTCAAGTGTTCAGCTTTTCACGATTGGCCTGGCGCGTCCTCCAGCATACGGGA
GGAATGAGCAGGCCGTTTCTTACGAGCACAGGCGTACAGATGCTCCTGCGGAAGCTCATTGAGGAGCATAAACAGGAGTT
TAAAGTCTATCAAAAAGCAAGTGATAAAAGCGGATTTACCGCACAAGTTGAGCGGATGCTGACAGAGTTTAAGCGGTATT
GTCTGGAACCGGAGGATATCCGCCGGATGGCGGAAAGCGGAACGGCTTCCGAGTATCGCGGAGAACGCGTTTTATCTGAA
AAGCTCCATGACTTATCGATTCTGTATCAGCAAATGGAAAAAAGTCTTGCAGATCAATATCTTCACTCTGAGGATTATTT
GACGTTGCTGGCAGAGCATATTCCGCTTGCGGAAGATATAAAAGGCGCGCATATCTATGTGGACGGCTTTTATCAGTTTA
CGCCGCAGGAATTCAGGGTGTTGGAGCAGCTTATGGTTCATGCAGAGCACATCACGTTTTCGCTGACAGCGGACAAGCCG
TCTTATGAGCGAGAGCCGCATGAACTGGAATTGTTCAGAATGACGGGGAAAACGTATTACCGCCTGCATCAGAAGGCGAA
GGAACTGAATTTGGACATTACCTATAAAGAGCTGAGCGGGATTGAGCGGCATACGCAGACGCCGGAATTGGCACATCTAG
AGGCGCAGTATGAAGCGCGCCCGGCCATCCCGTACGCAGAAAAACAAGAGGCCTTTACTGTGATGCAAGCCGCAAACAGA
CGAGCTGAGCTGGAAGGCATTGCCCGGGAAATTCACACTCTAGTCAGAGAGAAGGGCTATCGCTATAAAGATGTAGCGAT
TCTTGCGCGCCAGCCGGAAGACTACAAGGATATGGTGAAGGAAGTTTTCGCAGATTACGAGATTCCTTATTTCATTGACG
GAAAAGCATCTATGCTGAACCATCCGTTAATCGAATTTATCCGGTCGAGCATTGATGTTCTGAAAGGGAATTGGCGTTAT
GAAGCGGTGTTTCGCTGCGTGAAAACCGAACTGCTATTCCCGCTTAACGAGCCGAAGACTAAGGTGAGAGAACAGGTCGA
TCAGCTTGAAAATTACTGTATCGCCTACGGGATCAAAGGCGATCGCTGGACAAAGGGCGACCGTTTCCATTACAGACGTT
TTGTGTCATTGGATGATGATTTTGCGCAAACCGATCAGGAAATCGAAATGGAAAACATGCTGAATGACACACGCGACTGG
ATTGTTCCGCCGCTTTTTCAGTTTCAAAAACGAATGAAAAAAGCAAAGACGGTTCAGGAGAAGGCGGAGGCGCTCTACCG
CTATCTAGAAGAGACGGATGTACCGCTGAAGCTGGATCAGGAAAGACAGCGTGCTGAGGAAGACGGCAGAATCATTGAAG
CGCAGCAGCATCAGCAGGCGTGGGATGCAGTCATTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATT
TCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCA
GGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTC
TGCCTGCACGCCCTGATGAAAATGGGGTGCTGTCGGACGATGACCGGGAATGGCTGAAAACGATTGGGATTGAGCTTTCC
TCAGGCGGGCGGGAGCGTCTGCTTGATGAGCATTTCCTCATCTATATGGCGTTCTCCAGTCCGTCTGACCGGCTTTACGT
ATCGTATCCGATTGCTGATGCGGAAGGAAAAACGCTTTTGCCGTCGATTGTCATTAAACGGCTGGAAGAACTGTTTCCGC
AGCATAAGGAGCGGCTGTTGACAAATGAACCTGAACAGGTCAGCGATGAGGAACAGCTTATGTATGTCGTCAATAAAAGC
GTGGCACAGTCCTTTACCGCAAGCCAGCTCAGATTATGGACTCGGGAATACGACATCAGCGACGTCTGGTGGAGCACGTA
CAATGTGCTGATGAGTGAGCCGGACAGGCTGCAATCGAAAAAGCTGTTCTCAAGCCTGTTTTTCCGGAATGAAGTGAAGC
AGCTTGAACGCAGCGTGTCGAGACAGCTCTATGGTGAACGCATTCAGGGCAGTGTATCGAGAATGGAAACCTTTAACGCG
TGCCCGTTCTCCCATTTCGCATCACACGGGCTGCATCTAAAGGAACGGCAATTTTTCAAGCTCGAAGCACCGGATATCGG
CCAGCTGTTTCATTCCAGCTTAAAGCTGATTTCAGACCGGCTGCGCGAGCAAAAAATGAATTGGCGCGATTTAACGAAAG
AGCAGTGTGAACTGTTTTCCTATGATGCGGTAGAGCGGCTGGCACCGAAACTGCAAAAGGAAATTTTGCTCAGCTCAAAC
CGGCATTATTATGTGAAGGAAAAACTGCAAAAAATTGTGACACGTGTGTCCGGTATTTTAAGCGAGCATGCGAAAGCGAG
CGGGTTTGTGCCGATCGGGCTTGAACTGGGCTTTGGAGGAAAAGGGCCGCTTCCGCCGCTGACCTTTAAGCTGAAAAACG
GCTGCACGATGGAACTTGTCGGGCGAATTGACCGTGTTGATAAAGCAGAAAGCTCAAAAGGCCTGCTTCTAAGGATTGTC
GATTATAAATCTAGCGACAAAGGCCTTGACTTAGCGGAAGTATATTACGGACTGGCGCTGCAAATGCTGACGTACCTTGA
TTTATCGATTACTCATTCAGCCGACTGGCTCGGAATGAGGGCGACGCCGGCAGGAGTGCTGTATTTCCATATACATGACC
CGATGATTCAATCTAATCTCCCGCTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGGCTTG
CTCCTCGGCGATCAGGAAGCGGTTCGCCTTATGGATACGACCCTTCAAGAGGGACGGTCAAATATCATCAATGCGGGCTT
GAAAAAAGACGGCTCTCTCAGATCGGACTCAGCAGCAGTCGGCGAAAAGGAATTTGAGTTGTTGACGAAACATGTGCGCC
GCACCTTCCAAGAAGCAGGCGAACAAATCACCGACGGACGCGTATCGATTGAGCCTTATAAAATGAAGAACAAGACGCCG
TGCACATACTGTGCGTTCAAATCAGTATGCCAATTTGATGAATCATTAGAAGAGAACGAATATCGTCCGTTAAAGGCTGA
AAAGGATAAGACGATACTTGAGTGGATAAAAAAGGAGGCAGATGGCAATGAACATTCCTAA

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(790-1131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

98.113

100

0.981

Multiple sequence alignment