Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   EO946_RS00580 Genome accession   NZ_CP034943
Coordinates   105922..107298 (+) Length   458 a.a.
NCBI ID   WP_003225734.1    Uniprot ID   E0TYL6
Organism   Bacillus spizizenii ATCC 6633 = JCM 2499 strain ATCC 6633     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 100922..112298
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EO946_RS00560 (EO946_00560) ctsR 101275..101739 (+) 465 WP_003225724.1 transcriptional regulator CtsR -
  EO946_RS00565 (EO946_00565) mcsA 101753..102310 (+) 558 WP_003225726.1 protein-arginine kinase activator protein McsA -
  EO946_RS00570 (EO946_00570) - 102310..103401 (+) 1092 WP_003225728.1 protein arginine kinase -
  EO946_RS00575 (EO946_00575) clpC 103398..105830 (+) 2433 WP_003225731.1 ATP-dependent protease ATP-binding subunit ClpC Regulator
  EO946_RS00580 (EO946_00580) radA 105922..107298 (+) 1377 WP_003225734.1 DNA repair protein RadA Machinery gene
  EO946_RS00585 (EO946_00585) disA 107302..108384 (+) 1083 WP_003225736.1 DNA integrity scanning diadenylate cyclase DisA -
  EO946_RS00590 (EO946_00590) - 108498..109598 (+) 1101 WP_003225738.1 PIN/TRAM domain-containing protein -
  EO946_RS00595 (EO946_00595) ispD 109613..110311 (+) 699 WP_003225740.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  EO946_RS00600 (EO946_00600) ispF 110304..110780 (+) 477 WP_003225745.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 49442.88 Da        Isoelectric Point: 7.6840

>NTDB_id=333548 EO946_RS00580 WP_003225734.1 105922..107298(+) (radA) [Bacillus spizizenii ATCC 6633 = JCM 2499 strain ATCC 6633]
MAKTKSKFICQSCGYESPKWMGKCPGCGAWNTMVEEMIKKAPANRRAAFSHSVQTVQKPSPITSIETSEEPRVKTQLGEF
NRVLGGGVVKGSLVLIGGDPGIGKSTLLLQVSAQLSDSSNSVLYISGEESVKQTKLRADRLGINSPSLHVLSETDMEYIS
SAIQEMNPAFVVVDSIQTVYQSDITSAPGSVSQVRECTAELMKIAKTKGIPIFIVGHVTKEGSIAGPRLLEHMVDTVLYF
EGERHHTFRILRAVKNRFGSTNEMGIFEMREEGLTEVLNPSEIFLEERSAGSAGSSITASMEGTRPILVEIQALISPTSF
GNPRRMATGIDHNRVSLLMAVLEKRVGLLLQNQDAYLKVAGGVKLDEPAIDLAIAISIASSFRDTPPNPADCFIGEVGLT
GEVRRVSRIEQRVKEAAKLGFTRMIIPAANLDGWTKPKGIEVIGVANVAEALRTSLGG

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=333548 EO946_RS00580 WP_003225734.1 105922..107298(+) (radA) [Bacillus spizizenii ATCC 6633 = JCM 2499 strain ATCC 6633]
ATGGCTAAAACAAAATCGAAATTCATCTGCCAATCCTGCGGTTACGAGTCTCCAAAATGGATGGGGAAATGTCCGGGCTG
CGGTGCTTGGAATACTATGGTGGAAGAAATGATAAAAAAAGCGCCGGCCAATCGGAGAGCGGCTTTTTCTCATTCAGTTC
AAACTGTACAGAAACCTTCACCTATTACATCAATTGAAACATCAGAAGAACCCCGGGTTAAAACCCAGCTTGGCGAGTTT
AACAGAGTGCTCGGCGGGGGCGTCGTCAAAGGCTCACTCGTTTTAATCGGCGGCGATCCTGGTATCGGAAAGTCAACGTT
ATTACTGCAGGTTTCCGCTCAATTATCAGATTCATCGAACAGTGTGCTGTACATTTCGGGAGAAGAATCGGTAAAGCAAA
CGAAACTGAGAGCAGATCGTCTTGGCATTAATAGTCCGTCACTGCATGTTTTATCTGAAACCGATATGGAGTATATTTCG
TCTGCTATACAAGAGATGAATCCAGCGTTTGTCGTTGTAGACTCTATTCAAACGGTTTACCAAAGCGACATTACATCGGC
TCCGGGCAGTGTGTCACAGGTCAGAGAATGTACCGCTGAGCTGATGAAAATTGCAAAAACAAAAGGTATTCCGATTTTTA
TCGTAGGGCACGTGACGAAGGAAGGGTCGATTGCAGGGCCGAGACTGTTGGAGCATATGGTCGACACTGTTTTATATTTT
GAAGGGGAACGTCACCATACGTTCCGTATTTTGCGGGCTGTAAAAAACCGTTTTGGTTCTACAAATGAAATGGGCATTTT
TGAAATGCGCGAAGAGGGGCTCACTGAGGTTTTGAATCCTTCAGAAATTTTCTTAGAAGAACGTTCAGCGGGATCCGCAG
GCTCAAGCATAACTGCTTCTATGGAGGGCACAAGACCGATTCTCGTTGAAATCCAGGCGCTCATCTCGCCAACAAGCTTT
GGCAATCCGCGGCGTATGGCAACAGGAATAGACCATAACAGGGTTTCGCTGTTAATGGCTGTGTTAGAAAAAAGAGTTGG
GCTGCTGCTGCAAAATCAGGATGCTTATTTGAAAGTAGCCGGCGGCGTAAAGCTTGATGAACCGGCAATTGATCTTGCCA
TTGCGATCAGTATCGCGTCCAGCTTTAGAGATACACCTCCAAATCCTGCAGATTGTTTTATAGGAGAAGTGGGATTAACC
GGAGAAGTCCGCCGGGTTTCAAGAATTGAACAACGTGTGAAAGAAGCGGCTAAGCTTGGTTTTACACGCATGATCATACC
CGCGGCAAATCTGGATGGATGGACAAAACCAAAAGGGATTGAGGTTATCGGAGTAGCAAATGTTGCAGAGGCGCTTCGTA
CTTCATTAGGAGGATAA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(365-430)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(72-222)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB E0TYL6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

98.908

100

0.989
  radA Streptococcus pneumoniae Rx1

63.576

98.908

0.629
  radA Streptococcus pneumoniae D39

63.576

98.908

0.629
  radA Streptococcus pneumoniae R6

63.576

98.908

0.629
  radA Streptococcus pneumoniae TIGR4

63.576

98.908

0.629
  radA Streptococcus mitis NCTC 12261

63.576

98.908

0.629
  radA Streptococcus mitis SK321

63.355

98.908

0.627

Multiple sequence alignment