Detailed information    

insolico Bioinformatically predicted

Overview

Name   recF   Type   Machinery gene
Locus tag   EO946_RS00020 Genome accession   NZ_CP034943
Coordinates   3028..4140 (+) Length   370 a.a.
NCBI ID   WP_003219265.1    Uniprot ID   -
Organism   Bacillus spizizenii ATCC 6633 = JCM 2499 strain ATCC 6633     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1..9140
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EO946_RS00005 (EO946_00005) dnaA 1..1341 (+) 1341 WP_003219262.1 chromosomal replication initiator protein DnaA -
  EO946_RS00010 (EO946_00010) dnaN 1529..2665 (+) 1137 WP_003219263.1 DNA polymerase III subunit beta -
  EO946_RS00015 (EO946_00015) rlbA 2797..3012 (+) 216 WP_003219264.1 ribosome maturation protein RlbA -
  EO946_RS00020 (EO946_00020) recF 3028..4140 (+) 1113 WP_003219265.1 DNA replication/repair protein RecF Machinery gene
  EO946_RS00025 (EO946_00025) remB 4163..4408 (+) 246 WP_003219266.1 extracellular matrix regulator RemB -
  EO946_RS00030 (EO946_00030) gyrB 4463..6379 (+) 1917 WP_003219267.1 DNA topoisomerase (ATP-hydrolyzing) subunit B -
  EO946_RS00035 (EO946_00035) gyrA 6596..9061 (+) 2466 WP_003219268.1 DNA topoisomerase (ATP-hydrolyzing) subunit A -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 42347.41 Da        Isoelectric Point: 7.7139

>NTDB_id=333544 EO946_RS00020 WP_003219265.1 3028..4140(+) (recF) [Bacillus spizizenii ATCC 6633 = JCM 2499 strain ATCC 6633]
MYIQNLELTSYRNYDHAELQFENKVNVIIGENAQGKTNLMEAIYVLSMAKSHRTSNDKELIRWDKDYAKIEGRVMKQNGA
IPMQLVISKKGKKGKVNHIEQQKLSQYVGALNTIMFAPEDLNLVKGSPQVRRRFLDMEIGQVSPVYLHDLSLYQKILSQR
NHFLKQLQTRKQTDRTMLDVLTDQLVEVAAKVVVKRLQFTAQLEKWAQPIHAGISRGLEELTLKYHTALEVSDPKDLSKI
GDSYQEAFSKLREKEIERGITLSGPHRDDVLFYVNGRDVQTYGSQGQQRTTALSLKLAEIDLIHEEIGEYPILLLDDVLS
ELDDYRQSHLLHTIQGRVQTFVTTTSVDGIDHETLRQAGMFRVQNGALVK

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=333544 EO946_RS00020 WP_003219265.1 3028..4140(+) (recF) [Bacillus spizizenii ATCC 6633 = JCM 2499 strain ATCC 6633]
TTGTATATCCAGAACTTAGAACTGACATCTTACCGCAACTACGACCATGCTGAACTTCAATTTGAAAATAAAGTAAATGT
GATCATCGGAGAAAACGCCCAGGGGAAAACAAATCTCATGGAGGCGATCTATGTCTTGTCCATGGCGAAATCGCACAGGA
CGTCAAATGACAAAGAACTTATACGGTGGGACAAAGACTATGCTAAAATAGAGGGAAGAGTGATGAAGCAAAACGGGGCG
ATTCCCATGCAGCTCGTCATCTCCAAAAAGGGTAAAAAGGGCAAGGTCAATCATATTGAACAGCAAAAGCTCAGCCAGTA
TGTCGGGGCCCTCAACACCATTATGTTCGCGCCGGAAGATTTAAATCTTGTAAAGGGAAGCCCTCAAGTGAGAAGGCGGT
TTCTTGACATGGAAATCGGACAGGTTTCTCCTGTTTACCTGCATGACCTTTCTCTTTACCAGAAAATCCTCTCGCAGCGA
AATCATTTTTTGAAGCAGCTGCAAACAAGAAAACAAACTGACAGAACGATGCTCGATGTTTTAACCGATCAGCTTGTAGA
AGTTGCAGCAAAAGTCGTCGTAAAACGCCTGCAGTTTACAGCGCAGCTCGAAAAATGGGCTCAGCCTATCCATGCGGGCA
TCTCAAGAGGGCTTGAAGAGCTGACCCTGAAGTACCATACGGCTCTTGAGGTATCAGATCCTAAAGACTTGTCGAAAATA
GGAGATAGCTATCAAGAAGCGTTTTCTAAATTAAGAGAAAAAGAAATTGAGCGCGGTATTACGCTGTCAGGGCCTCACCG
TGACGATGTTCTTTTCTATGTGAACGGACGCGATGTGCAGACGTATGGTTCTCAAGGACAGCAGCGAACCACGGCATTGT
CCCTTAAGCTGGCGGAGATTGACCTGATCCATGAAGAAATCGGAGAATACCCCATTTTACTATTGGATGATGTACTGAGT
GAGCTGGATGATTATCGCCAGTCACACTTGCTTCATACGATCCAAGGCCGTGTACAAACGTTTGTCACAACGACAAGCGT
TGATGGCATTGATCACGAAACCTTACGTCAAGCAGGAATGTTCCGTGTGCAAAACGGTGCGTTAGTGAAGTGA

Domains

Predicted by InterproScan.

RecF/RecN/SMC N terminal domain (SMC_N)

(2-350)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recF Bacillus subtilis subsp. subtilis str. 168

99.189

100

0.992

Multiple sequence alignment