Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   VVMO6_RS02015 Genome accession   NC_014965
Coordinates   413962..415686 (+) Length   574 a.a.
NCBI ID   WP_011079397.1    Uniprot ID   A0A3Q0L3L1
Organism   Vibrio vulnificus MO6-24/O     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 408962..420686
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VVMO6_RS01990 (VVM_00700) gspM 409366..410016 (+) 651 WP_011079392.1 type II secretion system protein GspM -
  VVMO6_RS01995 (VVM_00701) - 410009..410329 (+) 321 WP_011079393.1 MSHA biogenesis protein MshK -
  VVMO6_RS02000 (VVM_00702) mshL 410345..411991 (+) 1647 WP_086016831.1 pilus (MSHA type) biogenesis protein MshL -
  VVMO6_RS02005 (VVM_00704) - 411999..412847 (+) 849 WP_013571060.1 ExeA family protein -
  VVMO6_RS02010 (VVM_00705) - 412844..413965 (+) 1122 WP_013571061.1 tetratricopeptide repeat protein -
  VVMO6_RS02015 (VVM_00707) pilF 413962..415686 (+) 1725 WP_011079397.1 GspE/PulE family protein Machinery gene
  VVMO6_RS02020 (VVM_00709) - 415696..416916 (+) 1221 WP_011079398.1 type II secretion system F family protein -
  VVMO6_RS02025 (VVM_00711) - 416920..417381 (+) 462 WP_013571062.1 hypothetical protein -
  VVMO6_RS02030 (VVM_00712) - 417460..418038 (+) 579 WP_013571063.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  VVMO6_RS02035 (VVM_00713) - 418072..418581 (+) 510 WP_013571064.1 type II secretion system protein -
  VVMO6_RS02040 (VVM_00714) - 418602..419096 (+) 495 WP_013571065.1 type II secretion system protein -
  VVMO6_RS02045 (VVM_00715) - 419096..419662 (+) 567 WP_013571066.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  VVMO6_RS02050 (VVM_00718) - 419662..420351 (+) 690 WP_013571067.1 PilW family protein -

Sequence

Protein


Download         Length: 574 a.a.        Molecular weight: 63672.03 Da        Isoelectric Point: 5.3226

>NTDB_id=33317 VVMO6_RS02015 WP_011079397.1 413962..415686(+) (pilF) [Vibrio vulnificus MO6-24/O]
MKVRLRKRLGDLLVEEGIINEAQLDQALGAQRSGGRKLGDTLIDLGFLSEQQMLAFLSQQLDLPLIDLNRATVDIDAVQL
LPEVHARRLRALVIGRNGDTLRIAMSDPADLFAQEALIGQLGQYGIEFVIAPERQLVEGFDRYYRRTKEIANFAEQLQAE
HQVNDAFDFNIEDDDSDEVTVVKLINSMFEDAIQVGASDIHIEPDANVLRLRQRIDGVLHETLLNEVNIAPALVLRLKLM
ANLDISEKRLPQDGRFNIRSKGQSVDIRMSTMPVQYGESVVMRLLNQSSGVRKLEDSGLPDDLLVRLRRQLKRPHGMILV
TGPTGSGKTTTLYGALTELNQPGKKIITAEDPVEYRLSRVNQVQINPKINLDFSTVLRTFLRQDPDIILIGEMRDHETVE
IGLRAALTGHLVLSTLHTNDAVDSALRMIDMGAPGYLVASAVRAVVAQRLVRRVCPDCKTEDHVGESRKAWLAQRFPNQV
EAGFVKGRGCQNCNLTGYRGRIGVFEMLELEHGMMDALRANDAVEFAHAARRSPGYKPLLASAMELALQGVVSLDEVMNL
GEGDSSGVAEAIIM

Nucleotide


Download         Length: 1725 bp        

>NTDB_id=33317 VVMO6_RS02015 WP_011079397.1 413962..415686(+) (pilF) [Vibrio vulnificus MO6-24/O]
ATGAAAGTAAGATTAAGAAAGCGACTTGGTGACCTTCTTGTTGAGGAAGGCATTATCAATGAAGCGCAACTTGATCAAGC
ACTCGGCGCGCAAAGAAGTGGTGGACGCAAGCTTGGCGATACGCTGATTGATTTGGGTTTTTTGTCTGAACAACAGATGC
TGGCGTTTTTGTCTCAGCAACTTGATCTGCCTTTAATTGACTTAAATCGAGCCACGGTCGATATCGATGCAGTGCAGTTG
TTACCAGAAGTGCATGCTCGTCGTTTACGCGCATTGGTGATTGGCCGTAATGGCGACACGTTGCGCATTGCGATGAGTGA
TCCAGCGGACCTTTTTGCGCAAGAAGCGTTGATCGGTCAGCTAGGACAATATGGCATTGAGTTTGTTATCGCGCCAGAAA
GACAATTGGTGGAAGGCTTTGATCGCTATTATCGCCGTACCAAAGAGATCGCCAACTTTGCCGAGCAGTTACAAGCGGAA
CACCAAGTCAACGATGCATTCGATTTCAATATCGAAGATGACGACAGTGATGAGGTGACCGTCGTTAAGCTCATCAACTC
GATGTTTGAAGACGCCATCCAGGTGGGCGCGTCGGATATCCATATCGAACCGGACGCCAATGTGTTGCGCTTGCGCCAAC
GTATTGATGGTGTGTTGCACGAAACCTTGCTGAATGAAGTCAACATCGCGCCAGCATTGGTACTGAGATTAAAACTCATG
GCCAACTTGGATATTTCAGAAAAACGTCTGCCGCAAGATGGCCGTTTTAACATCCGCTCCAAAGGTCAGTCGGTGGATAT
CCGTATGTCGACCATGCCAGTTCAGTATGGTGAGTCGGTGGTGATGCGTTTGCTCAATCAATCTTCCGGAGTCAGAAAAT
TAGAAGACTCCGGTTTGCCGGACGATCTTTTAGTGCGTCTGCGCCGACAGTTGAAACGTCCGCATGGCATGATCCTTGTT
ACTGGCCCTACGGGATCTGGTAAAACCACCACGCTGTATGGTGCTCTAACAGAATTAAACCAACCGGGGAAAAAGATCAT
CACAGCGGAAGATCCGGTTGAATACCGACTCTCGCGTGTCAACCAAGTGCAGATCAATCCGAAGATCAACCTTGATTTTT
CCACGGTGTTAAGAACGTTCCTCCGTCAGGATCCCGATATCATTTTGATTGGTGAGATGCGTGACCATGAAACGGTTGAA
ATTGGCCTGCGAGCGGCGCTGACCGGTCACTTAGTATTGAGTACCTTACACACCAATGACGCCGTAGACAGTGCGCTTCG
TATGATTGACATGGGGGCACCTGGTTATCTGGTCGCGAGTGCCGTACGTGCTGTGGTCGCGCAGCGATTGGTGCGCCGTG
TCTGTCCGGATTGCAAAACGGAAGATCATGTTGGCGAGTCACGCAAAGCGTGGTTGGCACAACGCTTCCCAAATCAAGTG
GAAGCGGGTTTTGTGAAAGGGCGCGGTTGCCAAAACTGTAACTTAACAGGCTATCGCGGCCGTATTGGTGTGTTTGAAAT
GCTGGAACTTGAACATGGAATGATGGACGCGTTGCGCGCCAATGATGCCGTCGAGTTTGCACATGCTGCGCGTCGTTCGC
CTGGCTATAAACCTCTGTTAGCGTCGGCAATGGAGTTGGCGCTGCAAGGCGTAGTGAGCTTAGACGAAGTGATGAATTTA
GGCGAAGGTGACTCCTCTGGGGTTGCTGAAGCGATCATTATGTAA

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-148)

Type II/IV secretion system protein (T2SSE)

(187-453)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3Q0L3L1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Thermus thermophilus HB27

38.104

97.387

0.371
  pilB Vibrio cholerae strain A1552

37.699

98.432

0.371

Multiple sequence alignment