Detailed information NTG0033310

Bioinformatically predicted

Overview

• Name: dprA/cilB/dalA
• Type: Machinery gene
• Locus tag: SP70585_RS06600
• Genome accession: NC_012468
• Coordinates: 1232812..1233660 (-)
• Length: 282 a.a.
• NCBI ID: WP_000705310.1
• Uniprot ID: C1C7P4
• Organism: Streptococcus pneumoniae 70585
• Function: ssDNA binding; loading RecA onto ssDNA; competence shut-off (predicted from homology) DNA processing Competence regulation

Genomic Context

Location: 1227812..1238660

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
SP70585_RS06585 (SP70585_1325)	-	1228166..1228525 (-)	360	WP_001237219.1	YbaN family protein	-
SP70585_RS06590 (SP70585_1326)	topA	1228631..1230718 (-)	2088	WP_000179641.1	type I DNA topoisomerase	-
SP70585_RS06595 (SP70585_1327)	-	1230885..1231928 (-)	1044	WP_015898771.1	DUF389 domain-containing protein	-
SP70585_RS14160 (SP70585_1328)	-	1232506..1232628 (+)	123	WP_001814242.1	hypothetical protein	-
SP70585_RS06600 (SP70585_1329)	dprA/cilB/dalA	1232812..1233660 (-)	849	WP_000705310.1	DNA-processing protein DprA	Machinery gene
SP70585_RS06605 (SP70585_1330)	-	1233756..1234445 (-)	690	WP_000643970.1	sugar phosphate nucleotidyltransferase	-
SP70585_RS06610 (SP70585_1331)	-	1234457..1235335 (-)	879	WP_000837609.1	DMT family transporter	-
SP70585_RS06615 (SP70585_1332)	licA	1235325..1236194 (-)	870	WP_000411199.1	choline kinase LicA	-
SP70585_RS06620 (SP70585_1333)	-	1236211..1237233 (-)	1023	WP_000609869.1	ribitol-5-phosphate dehydrogenase	-
SP70585_RS06625 (SP70585_1334)	-	1237238..1237945 (-)	708	WP_000638502.1	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase	-

Sequence

Protein

• Length: 282 a.a.
• Molecular weight: 31080.76 Da
• Isoelectric Point: 5.4047

>NTDB_id=33310 SP70585_RS06600 WP_000705310.1 1232812..1233660(-) (dprA/cilB/dalA) [Streptococcus pneumoniae 70585]
MKITNYEIYKLKKSGLTNQQILKVLEYGENVDQELLLGDIADISGCRNPAVFMERYFQIDDAHLSKEFQKFPSFSILDDC
YPWDLSEIYDAPVLLFYKGNLDLLKFPKVAVVGSRACSKQGAKSVEKVIQGLENELVIVSGMAKGIDTAAHMAALQNGGK
TIAVIGTGLDVFYPKANKRLQDYIGNDHLVLSEYGPGEQPLKFHFPARNRIIAGLCRGVIVAEAKMRSGSLITCERAMEE
GRDVFAIPGSILDGLSDGCHHLIQEGAKLVTSGQDVLAEFEF

Nucleotide

• Length: 849 bp

>NTDB_id=33310 SP70585_RS06600 WP_000705310.1 1232812..1233660(-) (dprA/cilB/dalA) [Streptococcus pneumoniae 70585]
ATGAAAATCACAAACTATGAAATCTATAAGTTAAAAAAATCAGGTTTGACCAATCAACAGATTTTGAAAGTGCTAGAATA
CGGTGAAAATGTTGATCAGGAGCTTTTATTGGGTGATATTGCAGATATCTCAGGTTGCCGTAATCCAGCCGTTTTTATGG
AACGTTATTTTCAGATAGACGATGCGCATTTGTCGAAAGAGTTTCAAAAATTTCCATCTTTCTCTATTTTAGATGACTGT
TATCCTTGGGATTTGAGTGAAATATATGATGCGCCTGTACTTTTATTTTACAAGGGAAATCTTGACCTCCTGAAATTCCC
GAAGGTAGCGGTCGTGGGCAGTCGTGCTTGTAGCAAACAGGGAGCTAAGTCAGTTGAAAAAGTCATTCAAGGCTTGGAAA
ATGAACTGGTTATTGTCAGTGGTATGGCCAAGGGCATTGACACAGCAGCTCATATGGCAGCTCTTCAGAATGGCGGAAAA
ACCATTGCAGTGATTGGAACAGGACTGGATGTGTTTTATCCTAAAGCCAATAAACGCTTGCAAGACTACATCGGCAATGA
CCATCTGGTTCTAAGTGAATATGGACCTGGCGAACAACCTCTGAAATTTCATTTTCCTGCCCGTAATCGCATCATTGCTG
GACTTTGTCGTGGTGTGATTGTAGCAGAGGCTAAGATGCGTTCAGGTAGTCTCATTACGTGTGAGCGAGCAATGGAAGAA
GGACGCGATGTCTTTGCTATTCCTGGTAGCATTTTAGATGGACTATCAGACGGTTGCCATCATTTGATTCAAGAAGGAGC
AAAATTGGTCACCAGTGGGCAAGATGTTCTTGCGGAATTTGAATTTTAA

Domains

Predicted by InterproScan.

• DNA recombination-mediator protein A (DNA_processg_A) (75-271)

• DNA processing protein A sterile alpha motif domain (SAM_DprA) (3-64)

3D structure

• AlphaFold DB: C1C7P4

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
dprA/cilB/dalA	Streptococcus pneumoniae Rx1	99.645	100	0.996
dprA/cilB/dalA	Streptococcus pneumoniae D39	99.645	100	0.996
dprA/cilB/dalA	Streptococcus pneumoniae R6	99.645	100	0.996
dprA/cilB/dalA	Streptococcus pneumoniae TIGR4	99.645	100	0.996
dprA/cilB/dalA	Streptococcus mitis NCTC 12261	98.227	100	0.982
dprA/cilB/dalA	Streptococcus mitis SK321	97.518	100	0.975
dprA	Streptococcus mutans UA159	62.857	99.291	0.624
dprA	Lactococcus lactis subsp. cremoris KW2	55.357	99.291	0.55
dprA	Staphylococcus aureus N315	37.456	100	0.376