Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   FVF73_RS00740 Genome accession   NZ_CP042934
Coordinates   158000..158440 (-) Length   146 a.a.
NCBI ID   WP_000360895.1    Uniprot ID   B7LFX5
Organism   Escherichia coli 042     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 153000..163440
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FVF73_RS00720 (FVF73_00720) coaE 153490..154110 (-) 621 WP_001269520.1 dephospho-CoA kinase -
  FVF73_RS23565 - 154135..154179 (+) 45 WP_120795372.1 protein YacM -
  FVF73_RS00725 (FVF73_00725) guaC 154335..155378 (+) 1044 WP_001217342.1 GMP reductase -
  FVF73_RS00730 (FVF73_00730) hofC 155413..156615 (-) 1203 WP_000157227.1 protein transport protein HofC -
  FVF73_RS00735 (FVF73_00735) pilB 156605..157990 (-) 1386 WP_001025173.1 type II secretion system protein GspE Machinery gene
  FVF73_RS00740 (FVF73_00740) pilA 158000..158440 (-) 441 WP_000360895.1 prepilin peptidase-dependent pilin Machinery gene
  FVF73_RS00745 (FVF73_00745) nadC 158643..159536 (-) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  FVF73_RS00750 (FVF73_00750) ampD 159624..160175 (+) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  FVF73_RS00755 (FVF73_00755) ampE 160172..161026 (+) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  FVF73_RS00760 (FVF73_00760) aroP 161069..162442 (-) 1374 WP_000969915.1 aromatic amino acid transporter AroP -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15602.71 Da        Isoelectric Point: 4.3927

>NTDB_id=333092 FVF73_RS00740 WP_000360895.1 158000..158440(-) (pilA) [Escherichia coli 042]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTH
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=333092 FVF73_RS00740 WP_000360895.1 158000..158440(-) (pilA) [Escherichia coli 042]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCAC
TATGTTTCAGCCATGAGTGTGGCAAAAGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGACTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7LFX5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363