Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   ELQ88_RS04470 Genome accession   NZ_CP034783
Coordinates   535890..537968 (+) Length   692 a.a.
NCBI ID   WP_138963861.1    Uniprot ID   A0A5B7VTF7
Organism   Pseudomonas sp. MPC6     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 530890..542968
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ELQ88_RS04450 (ELQ88_04450) comM 533105..534169 (+) 1065 WP_138963855.1 pilus assembly protein PilM Machinery gene
  ELQ88_RS04455 (ELQ88_04455) - 534169..534732 (+) 564 WP_128872934.1 PilN domain-containing protein -
  ELQ88_RS04460 (ELQ88_04460) pilO 534729..535352 (+) 624 WP_138963857.1 type 4a pilus biogenesis protein PilO -
  ELQ88_RS04465 (ELQ88_04465) - 535349..535876 (+) 528 WP_138963859.1 pilus assembly protein PilP -
  ELQ88_RS04470 (ELQ88_04470) pilQ 535890..537968 (+) 2079 WP_138963861.1 type IV pilus secretin PilQ Machinery gene
  ELQ88_RS04475 (ELQ88_04475) aroK 537973..538491 (+) 519 WP_128872930.1 shikimate kinase AroK -
  ELQ88_RS04480 (ELQ88_04480) aroB 538642..539742 (+) 1101 WP_128872929.1 3-dehydroquinate synthase -
  ELQ88_RS04485 (ELQ88_04485) - 539753..541342 (+) 1590 WP_138963863.1 AAA family ATPase -

Sequence

Protein


Download         Length: 692 a.a.        Molecular weight: 75109.46 Da        Isoelectric Point: 5.4266

>NTDB_id=332986 ELQ88_RS04470 WP_138963861.1 535890..537968(+) (pilQ) [Pseudomonas sp. MPC6]
MIRIFSTFGISLWIALLSPMVSAANLKTLDVAALPGDRVELKLSFDGPPPTPRGYTTESPARIALDLPGVVSQLKSKNRE
LGGGNARSATVVEAKDRTRLIINLTQLTPYDARVEGNTLFVVVGQGAAKAAPRPANTPRAATATPAPARTYAPVGKAIRG
VDFQRGTQGEGNVVIDLSDPTIAPDIQERDGKIILGFARTQLPEPLRVRLDVKDFATPVQFVNASATGDRATISIEPSGA
FDYSTYQTDNKLTVSIRPMTVDDLQKRNAERFTYTGEKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGITLRLQNV
PWDQALDLVLKTKGLDKRMIGNVLLVAPADEIAARERQELESQKQIAELAPLRRELLQVNYAKAADIAKLFQSVTSAEAK
IDERGSITVDERTNNIIAYQTQDRLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSVQNKGNWNTSGVSN
GSSTTIGTPGSTSTNSPFVDMGTAGNTSGLGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTEIPY
QEASSSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQDVPPIKKNEVNAKVLVNDGETIVIGGVFSN
TQSKVVDKVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2079 bp        

>NTDB_id=332986 ELQ88_RS04470 WP_138963861.1 535890..537968(+) (pilQ) [Pseudomonas sp. MPC6]
ATGATCAGGATTTTCTCCACCTTCGGTATTTCGCTATGGATAGCGCTGCTGTCGCCGATGGTATCAGCGGCCAACCTCAA
GACGCTGGATGTCGCGGCGTTACCGGGAGACCGCGTCGAGTTGAAGTTGTCCTTCGATGGACCGCCCCCGACGCCCCGTG
GCTACACCACGGAATCGCCGGCACGGATTGCGCTGGATCTGCCCGGGGTAGTCAGTCAGCTGAAGAGCAAGAATCGCGAA
CTGGGGGGCGGCAATGCCCGCAGCGCCACGGTGGTGGAGGCCAAGGACCGCACGCGGCTGATCATCAACCTCACTCAATT
GACCCCGTATGACGCGCGGGTCGAAGGCAACACCCTGTTCGTGGTTGTCGGGCAAGGCGCCGCGAAGGCCGCACCCCGGC
CCGCCAATACGCCTCGCGCTGCCACTGCAACGCCAGCCCCGGCCAGGACGTATGCGCCGGTGGGCAAGGCCATACGGGGC
GTGGATTTCCAGCGCGGCACCCAGGGTGAAGGCAATGTGGTCATCGATCTGTCGGACCCCACCATCGCCCCGGATATTCA
GGAGCGCGACGGCAAGATCATCCTCGGTTTCGCCAGGACACAACTGCCCGAGCCATTGCGGGTGCGCCTGGACGTCAAGG
ACTTCGCCACGCCGGTGCAGTTCGTCAATGCCAGCGCCACGGGCGATCGGGCCACCATCAGCATCGAGCCCAGCGGCGCG
TTTGACTATTCGACCTATCAAACCGACAACAAGCTGACGGTCAGCATCCGGCCGATGACCGTCGACGACCTGCAAAAACG
CAACGCCGAGCGTTTCACCTACACCGGTGAAAAGCTCTCGCTGAATTTCCAGGACATCGATGTGCGCTCGGTGCTGCAAC
TGATCGCTGATTTCACCAACCTCAACCTGGTGGCCAGCGACACGGTGCAGGGCGGGATCACCCTGCGTTTGCAGAACGTG
CCGTGGGACCAGGCGCTGGACCTGGTGCTGAAAACCAAAGGCCTGGATAAACGCATGATCGGCAACGTGCTGCTGGTGGC
GCCCGCCGATGAGATTGCCGCGCGCGAACGCCAGGAGCTCGAGTCGCAAAAGCAGATTGCCGAACTGGCGCCTTTGCGTC
GCGAGCTGCTGCAGGTCAACTACGCCAAGGCCGCGGATATTGCCAAGCTGTTCCAGTCGGTGACCAGTGCCGAGGCAAAA
ATCGACGAACGGGGCTCGATCACCGTCGATGAGCGCACCAACAACATCATTGCCTACCAGACCCAGGACCGCCTTGACGA
GCTGCGCCGTATCGTTGCGCAACTGGATATTCCGGTGCGGCAGGTGATGATCGAGGCGCGCATCGTCGAAGCCAATGTCG
ATTACGACAAAAGCCTCGGCGTGCGCTGGGGCGGTTCGGTGCAGAACAAAGGCAACTGGAACACGTCCGGGGTCAGCAAC
GGCTCGTCGACCACCATTGGTACGCCGGGCAGTACCAGCACCAACTCGCCGTTCGTCGACATGGGCACCGCCGGCAACAC
CTCGGGACTGGGCATCGCGTTCATCACCGACAATGTGTTGCTGGACCTGGAGCTGACCGCGATGGAGAAGACCGGCAACG
GCGAAATCGTCTCGCAGCCCAAGGTGGTCACATCCGACAAGGAAACCGCGAAAATCCTCAAAGGCACCGAAATTCCCTAT
CAGGAAGCCAGCTCCAGCGGCGCCACGTCGGTGTCGTTCAAGGAGGCCTCGCTGTCGCTGGAAGTGACGCCGCAGATCAC
CCCGGACAACCGGATCATCATGGAGGTCAAGGTCACCAAGGACGAGCCGGACTACTTGAACAAGGTGCAGGATGTGCCGC
CGATCAAGAAAAACGAGGTCAATGCCAAGGTGCTGGTCAATGACGGCGAGACCATCGTCATTGGTGGCGTTTTTTCAAAT
ACTCAGAGCAAGGTTGTAGATAAGGTGCCATTTCTTGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCGTGACGTGGT
TTCGGAGAAAAAATCCGAGCTGTTGGTGTTTCTCACTCCGCGTATCATGAATAACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(302-348)

Bacterial type II and III secretion system protein (Secretin)

(527-683)

AMIN domain (AMIN)

(37-122)

Bacterial type II/III secretion system short domain (Secretin_N)

(376-441)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5B7VTF7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

73.558

100

0.756
  comQ Acinetobacter baylyi ADP1

40.385

100

0.425
  pilQ Acinetobacter baumannii D1279779

38.204

100

0.412
  pilQ Acinetobacter baumannii strain A118

38.204

100

0.412
  pilQ Legionella pneumophila strain ERS1305867

37.593

97.254

0.366
  pilQ Legionella pneumophila strain Lp02

37.593

97.254

0.366

Multiple sequence alignment