Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   ELK40_RS20145 Genome accession   NZ_CP034769
Coordinates   4045938..4047371 (+) Length   477 a.a.
NCBI ID   WP_111964974.1    Uniprot ID   -
Organism   Enterobacter sp. N18-03635     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4040938..4052371
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ELK40_RS20115 (ELK40_20115) - 4041505..4042482 (+) 978 WP_039260412.1 calcium/sodium antiporter -
  ELK40_RS20120 (ELK40_20120) kdsD 4042496..4043482 (+) 987 WP_127732414.1 arabinose-5-phosphate isomerase KdsD -
  ELK40_RS20125 (ELK40_20125) kdsC 4043497..4044063 (+) 567 WP_048981180.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  ELK40_RS20130 (ELK40_20130) lptC 4044060..4044635 (+) 576 WP_115876356.1 LPS export ABC transporter periplasmic protein LptC -
  ELK40_RS20135 (ELK40_20135) lptA 4044604..4045158 (+) 555 WP_033146750.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  ELK40_RS20140 (ELK40_20140) lptB 4045165..4045890 (+) 726 WP_023309359.1 LPS export ABC transporter ATP-binding protein -
  ELK40_RS20145 (ELK40_20145) rpoN/rpoN1 4045938..4047371 (+) 1434 WP_111964974.1 RNA polymerase factor sigma-54 Machinery gene
  ELK40_RS20150 (ELK40_20150) hpf 4047394..4047681 (+) 288 WP_111964975.1 ribosome hibernation promoting factor -
  ELK40_RS20155 (ELK40_20155) ptsN 4047764..4048255 (+) 492 WP_008501443.1 PTS IIA-like nitrogen regulatory protein PtsN -
  ELK40_RS20160 (ELK40_20160) rapZ 4048301..4049155 (+) 855 WP_008501442.1 RNase adapter RapZ -
  ELK40_RS20165 (ELK40_20165) npr 4049152..4049424 (+) 273 WP_003861872.1 PTS phosphocarrier protein NPr -
  ELK40_RS20170 (ELK40_20170) mtgA 4049464..4050189 (-) 726 WP_127732416.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  ELK40_RS20175 (ELK40_20175) elbB 4050186..4050839 (-) 654 WP_127732840.1 isoprenoid biosynthesis glyoxalase ElbB -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 54139.91 Da        Isoelectric Point: 4.3332

>NTDB_id=332951 ELK40_RS20145 WP_111964974.1 4045938..4047371(+) (rpoN/rpoN1) [Enterobacter sp. N18-03635]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALDSNPLLEQTDLHDEVDTQQSQDTEALDTADALEQKEMPD
ELPLDASWDEIYTAGTPSGTRADYQDDELPVYQGETTQSLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVTLDD
ILESMGDEEIELEEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFSKETPWLDEARVIISDHLDLLANHDFRTLMRVTR
LKEDVLKEAVNLIQSLDPRPGQSIQTSEPEYVIPDVLVRKHNGRWVVELNSDSIPRLQINQQYASMCTSVRNDSDNQYIR
SNLQEARWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTTMLSDQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=332951 ELK40_RS20145 WP_111964974.1 4045938..4047371(+) (rpoN/rpoN1) [Enterobacter sp. N18-03635]
ATGAAGCAAGGTTTGCAATTAAGGCTCAGCCAACAACTGGCGATGACGCCGCAGCTACAGCAGGCAATTCGCCTGTTGCA
ACTGTCCACGTTAGAACTTCAGCAGGAGCTCCAGCAGGCGCTGGACAGCAACCCCCTGCTGGAACAAACCGATCTTCATG
ACGAGGTAGATACTCAGCAGTCACAGGACACTGAAGCGCTTGATACCGCCGACGCACTCGAACAAAAAGAGATGCCCGAC
GAGCTTCCGCTGGATGCCAGCTGGGATGAAATCTACACCGCCGGAACGCCTTCCGGTACGCGTGCGGACTACCAGGATGA
TGAGCTACCGGTCTATCAGGGAGAAACCACCCAGTCGCTGCAGGATTACCTGATGTGGCAGGTGGAACTCACTCCCTTCT
CCGATACCGACCGCGCGATTGCCACCTCAATTGTCGATGCCGTTGACGACACCGGCTATTTGACCGTCACGCTTGACGAT
ATTCTGGAAAGCATGGGCGACGAAGAGATAGAGCTTGAGGAAGTTGAAGCCGTTCTGAAGCGCATCCAGCGTTTCGATCC
GGTAGGCGTAGCCGCAAAAGATCTGCGCGACTGCCTGCTAATCCAGCTTTCTCAGTTCAGCAAAGAGACGCCATGGCTCG
ACGAGGCGCGCGTGATTATCAGCGATCACCTGGATCTGCTGGCGAACCACGATTTCCGCACCCTGATGCGCGTCACGCGC
CTGAAAGAAGATGTGCTGAAAGAAGCGGTGAATCTGATCCAGTCACTCGATCCACGCCCGGGACAGTCAATCCAGACCAG
CGAGCCTGAGTACGTTATTCCGGACGTGCTGGTCAGAAAACACAATGGCCGCTGGGTCGTTGAACTGAATTCAGACAGCA
TCCCTCGCCTGCAAATCAACCAGCAGTATGCCTCCATGTGCACCAGCGTGCGTAACGACTCCGACAATCAATATATTCGT
AGCAATCTTCAGGAAGCGCGATGGCTGATCAAAAGTCTTGAGAGCCGAAATGATACGCTGCTACGCGTCAGCCGCTGTAT
TGTCGAACAGCAGCAGGCTTTCTTTGAGCAGGGCGAAGAGTATATGAAACCGATGGTGCTGGCGGATATCGCCCAGGCCG
TCGAGATGCATGAATCAACCATTTCCCGTGTCACCACGCAGAAGTATCTGCATAGTCCACGCGGTATTTTTGAGCTTAAG
TATTTCTTCTCCAGCCATGTGAATACCGAGGGCGGCGGCGAAGCGTCGTCAACGGCCATTCGTGCCCTGGTGAAGAAGTT
GATCGCCGCGGAGAACCCCGCGAAGCCGCTAAGCGACAGTAAGTTAACCACCATGCTGTCCGATCAGGGTATTATGGTGG
CACGTCGTACTGTTGCGAAGTATCGAGAGTCTTTATCCATTCCGCCGTCCAACCAGCGTAAACAGCTGGTCTGA

Domains

Predicted by InterProScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.545

100

0.482

Multiple sequence alignment