Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   EKN56_RS16210 Genome accession   NZ_CP034752
Coordinates   3631865..3633295 (+) Length   476 a.a.
NCBI ID   WP_130592742.1    Uniprot ID   A0A411WNP2
Organism   Limnobaculum zhutongyuii strain CF-458     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3626865..3638295
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EKN56_RS16180 (EKN56_16180) - 3627329..3628297 (+) 969 WP_130592738.1 calcium/sodium antiporter -
  EKN56_RS16185 (EKN56_16185) kdsD 3628362..3629330 (+) 969 WP_130592739.1 arabinose-5-phosphate isomerase KdsD -
  EKN56_RS16190 (EKN56_16190) kdsC 3629382..3629930 (+) 549 WP_130592740.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  EKN56_RS16195 (EKN56_16195) lptC 3629933..3630526 (+) 594 WP_130593742.1 LPS export ABC transporter periplasmic protein LptC -
  EKN56_RS16200 (EKN56_16200) lptA 3630504..3631025 (+) 522 WP_130593743.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  EKN56_RS16205 (EKN56_16205) lptB 3631068..3631793 (+) 726 WP_130592741.1 LPS export ABC transporter ATP-binding protein -
  EKN56_RS16210 (EKN56_16210) rpoN/rpoN1 3631865..3633295 (+) 1431 WP_130592742.1 RNA polymerase factor sigma-54 Machinery gene
  EKN56_RS16215 (EKN56_16215) ptsN 3633313..3633825 (+) 513 WP_130592743.1 PTS IIA-like nitrogen regulatory protein PtsN -
  EKN56_RS16220 (EKN56_16220) rapZ 3633958..3634830 (+) 873 WP_130592744.1 RNase adapter RapZ -
  EKN56_RS16225 (EKN56_16225) rnk 3635157..3635552 (+) 396 WP_130592745.1 nucleoside diphosphate kinase regulator -
  EKN56_RS16230 (EKN56_16230) - 3635618..3636160 (-) 543 WP_246019852.1 CDP-archaeol synthase -
  EKN56_RS16235 (EKN56_16235) - 3636150..3636773 (-) 624 WP_130592746.1 phosphatidylcholine/phosphatidylserine synthase -

Sequence

Protein


Download         Length: 476 a.a.        Molecular weight: 53475.06 Da        Isoelectric Point: 4.4460

>NTDB_id=332839 EKN56_RS16210 WP_130592742.1 3631865..3633295(+) (rpoN/rpoN1) [Limnobaculum zhutongyuii strain CF-458]
MKQGLQLKLSQQLTMTPQLQQAIRLLQLSTLELQQEIQIALESNPLLEQADNHDEIDSHEETEHEQLDTSEALQQSDIPE
DLPLDASWDEIYTAGTPSGTHNDYGDDELPVFQGETTLTLQDHLMWQMDLTPFSDLDKAIATAIIDAVDNTGYLTVSLED
IADSLGQDEVELDEIEAVLKRIQRFDPVGVAARDLRECLLVQLSQLSSDTPYLNDARQIADHHLALLGSHDFRSLLKLTR
ISEEALKGAMQLIQSLDPRPGQSIHASESEYVIPDVLVRKVQGKWVAELNTESIPRLQINQHYASMANTHNASDNQYIRT
NLQDARWLIKSLESRNDTLLRVTECIVERQEEFFELGAEYMKPMVLADIAQAVDMHESTISRVTTQKYLHSPRGIFELKY
FFSSHVNTDSGGEASSTAIRALVKKLISAENPGKPLSDSKIASMLTDQGIIVARRTVAKYRESLSIPPSNQRKQLI

Nucleotide


Download         Length: 1431 bp        

>NTDB_id=332839 EKN56_RS16210 WP_130592742.1 3631865..3633295(+) (rpoN/rpoN1) [Limnobaculum zhutongyuii strain CF-458]
ATGAAGCAAGGTTTACAGCTCAAGTTAAGTCAACAATTAACCATGACACCTCAGTTACAGCAGGCCATTCGCCTGTTGCA
ACTGTCGACACTTGAACTGCAACAAGAGATTCAAATCGCACTGGAAAGTAACCCACTGCTTGAGCAGGCGGATAATCATG
ACGAAATAGATTCGCATGAAGAGACCGAACACGAACAGCTCGATACCAGTGAAGCGCTACAACAGTCCGATATTCCTGAA
GACCTGCCCCTCGACGCCTCATGGGACGAAATTTATACCGCAGGAACACCGTCCGGCACCCATAACGATTATGGCGATGA
CGAACTCCCGGTGTTTCAGGGTGAAACCACCCTGACCCTGCAAGATCATCTGATGTGGCAGATGGATCTAACCCCCTTCT
CCGATCTGGATAAAGCCATTGCTACCGCCATTATTGATGCGGTGGACAATACCGGCTATCTGACGGTTTCGCTGGAAGAT
ATTGCCGACAGTCTGGGACAGGACGAAGTTGAACTCGACGAAATTGAAGCGGTGCTGAAGCGCATTCAGCGTTTCGACCC
GGTGGGCGTTGCTGCCCGCGATCTGCGAGAATGTTTGCTGGTGCAGCTTTCCCAGCTATCTTCTGATACCCCTTATCTGA
ATGATGCTCGTCAGATTGCCGACCATCATCTGGCACTGTTAGGCAGCCATGATTTCCGCTCATTACTAAAACTGACTCGA
ATTAGTGAAGAGGCACTGAAAGGGGCCATGCAACTGATTCAGAGCCTTGATCCTCGCCCTGGTCAGTCAATTCATGCCAG
CGAGTCCGAATACGTTATTCCTGATGTGCTGGTGCGCAAAGTTCAGGGCAAATGGGTGGCTGAGCTCAATACGGAAAGTA
TCCCACGCTTGCAGATTAACCAGCATTACGCCTCAATGGCAAATACCCATAATGCCAGCGATAATCAATATATCCGTACC
AATTTGCAAGATGCCCGCTGGCTAATCAAAAGCCTTGAAAGTCGTAACGACACGCTACTTAGGGTAACGGAATGTATTGT
AGAACGGCAGGAAGAATTTTTTGAGCTGGGCGCAGAATACATGAAGCCAATGGTTTTGGCAGATATCGCCCAAGCGGTTG
ATATGCATGAGTCAACCATCTCACGCGTAACAACCCAAAAGTACTTACATAGCCCCAGAGGGATATTTGAGTTAAAATAC
TTCTTTTCCAGTCACGTCAATACCGATAGCGGCGGCGAAGCCTCTTCTACCGCAATACGTGCACTGGTTAAAAAATTGAT
TTCGGCTGAAAATCCAGGTAAGCCGCTGAGCGACAGTAAAATCGCCAGCATGCTAACCGACCAAGGGATTATTGTTGCCA
GAAGGACGGTAGCAAAATACAGGGAGTCGTTATCTATTCCCCCGTCAAATCAGCGTAAACAACTGATCTGA

Domains

Predicted by InterProScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(117-303)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(316-474)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.736

100

0.496

Multiple sequence alignment