Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   CA902_RS18680 Genome accession   NZ_CP042896
Coordinates   3941162..3941602 (+) Length   146 a.a.
NCBI ID   WP_000360914.1    Uniprot ID   A0A0H2YVZ3
Organism   Escherichia coli strain CFSAN061771     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3936162..3946602
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CA902_RS18660 (CA902_018800) aroP 3937160..3938533 (+) 1374 WP_000969915.1 aromatic amino acid transporter AroP -
  CA902_RS18665 (CA902_018805) ampE 3938576..3939430 (-) 855 WP_000172019.1 beta-lactamase regulator AmpE -
  CA902_RS18670 (CA902_018810) ampD 3939427..3939978 (-) 552 WP_022645179.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  CA902_RS18675 (CA902_018815) nadC 3940066..3940959 (+) 894 WP_001135167.1 carboxylating nicotinate-nucleotide diphosphorylase -
  CA902_RS18680 (CA902_018820) pilA 3941162..3941602 (+) 441 WP_000360914.1 prepilin peptidase-dependent pilin Machinery gene
  CA902_RS18685 (CA902_018825) pilB 3941612..3942997 (+) 1386 WP_022645178.1 type II secretion system protein GspE Machinery gene
  CA902_RS18690 (CA902_018830) hofC 3942987..3944189 (+) 1203 WP_022645177.1 protein transport protein HofC -
  CA902_RS18695 (CA902_018835) guaC 3944224..3945267 (-) 1044 WP_001217337.1 GMP reductase -
  CA902_RS18700 - 3945423..3945467 (-) 45 WP_120795372.1 protein YacM -
  CA902_RS18705 (CA902_018840) coaE 3945492..3946112 (+) 621 WP_001269517.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15620.77 Da        Isoelectric Point: 4.5237

>NTDB_id=332749 CA902_RS18680 WP_000360914.1 3941162..3941602(+) (pilA) [Escherichia coli strain CFSAN061771]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLNTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=332749 CA902_RS18680 WP_000360914.1 3941162..3941602(+) (pilA) [Escherichia coli strain CFSAN061771]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAAATACCTGCGACGGTGGCAGCAATGGCATTCCTTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACGCCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2YVZ3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363