Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   ELS24_RS00635 Genome accession   NZ_CP034689
Coordinates   137071..138516 (+) Length   481 a.a.
NCBI ID   WP_050448874.1    Uniprot ID   A0AAW3HYU9
Organism   Achromobacter spanius strain UQ283     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 132071..143516
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ELS24_RS00620 (ELS24_00620) - 132884..134212 (+) 1329 WP_127183108.1 amidase -
  ELS24_RS00625 (ELS24_00625) leuA 134368..136080 (-) 1713 WP_127183109.1 2-isopropylmalate synthase -
  ELS24_RS00630 (ELS24_00630) - 136440..137072 (-) 633 WP_050448873.1 hypothetical protein -
  ELS24_RS00635 (ELS24_00635) rpoN/rpoN1 137071..138516 (+) 1446 WP_050448874.1 RNA polymerase factor sigma-54 Machinery gene
  ELS24_RS00640 (ELS24_00640) - 138638..138919 (+) 282 WP_050448875.1 (2Fe-2S)-binding protein -
  ELS24_RS00645 (ELS24_00645) bfr 139396..139872 (+) 477 WP_050448876.1 bacterioferritin -
  ELS24_RS00650 (ELS24_00650) - 139963..140649 (-) 687 WP_127183110.1 hypothetical protein -
  ELS24_RS00655 (ELS24_00655) - 140674..142281 (-) 1608 WP_050448878.1 peptide chain release factor 3 -
  ELS24_RS00660 (ELS24_00660) - 142395..143024 (-) 630 WP_127183111.1 LysE family transporter -

Sequence

Protein


Download         Length: 481 a.a.        Molecular weight: 52542.82 Da        Isoelectric Point: 4.8503

>NTDB_id=332561 ELS24_RS00635 WP_050448874.1 137071..138516(+) (rpoN/rpoN1) [Achromobacter spanius strain UQ283]
MQNIVPMTRPILELRPGQHLTLTPQLQQSIRLLQLSTLDLEAEISQVLADNPLLEREDDTPAAEAQAESERESSVQDDEP
AVERDSPAEEMPGSGGVYSDDDSGLPEAARPDTLREHLLGQLMLTRAAPRDAALAALLIDELDENGYLGSPLEEILTWLP
ADMEPDLDELRAALSLLQSFDPPGIGARDMADCLVLQLRHPDLSRLPEAADASVLACARAICAQHLPVLATGNAARLCAA
VGCDEATFRAAHTLILRLEPRPGRAWTVPAADYAVPDVIVRKTRRGWQVTLNSAAVPRLQINGLYAQMLGNQKESAHAGL
QTQLQQARWMIRNVEQRFDTILRVAQAIVERQTAFFSQGPSAMRPLILKDIAGELGLHESTISRATTQKYMLTPFGTLEL
KRFFGTGVSTDGGDATSATAVQTFIRQMVAEENRAKPLSDSQIMQKLADQGIVIARRTVAKYREALRIAPATLRKAQASA
R

Nucleotide


Download         Length: 1446 bp        

>NTDB_id=332561 ELS24_RS00635 WP_050448874.1 137071..138516(+) (rpoN/rpoN1) [Achromobacter spanius strain UQ283]
ATGCAAAATATTGTACCTATGACCCGTCCAATTTTAGAACTGCGACCTGGCCAGCATTTGACGCTGACCCCTCAGCTACA
GCAATCGATCCGATTGTTGCAGTTGTCCACGCTCGACCTCGAGGCCGAGATCTCGCAAGTGCTGGCGGACAACCCGCTGC
TGGAGCGGGAAGACGACACCCCCGCCGCCGAGGCGCAAGCCGAATCCGAGCGCGAGTCCTCCGTGCAGGACGATGAACCC
GCCGTCGAACGAGATTCCCCCGCCGAAGAAATGCCTGGGTCTGGCGGGGTGTATTCGGACGATGATTCCGGCCTGCCCGA
GGCGGCGCGCCCCGACACCCTGCGCGAGCACCTGTTGGGCCAACTGATGTTGACGCGGGCCGCCCCGCGCGACGCCGCGC
TGGCAGCCCTGCTGATCGACGAGCTGGACGAAAACGGGTATCTGGGATCGCCGCTTGAGGAAATCCTGACGTGGCTACCC
GCCGACATGGAACCGGACCTGGACGAATTGCGCGCTGCGCTGTCCTTGTTGCAGTCGTTCGACCCCCCTGGCATCGGTGC
GCGCGACATGGCCGACTGCCTGGTGTTGCAGTTGCGCCACCCTGACCTGAGCCGTCTGCCGGAAGCGGCGGACGCATCGG
TCCTGGCGTGCGCGCGCGCGATCTGCGCACAGCATCTGCCCGTGCTGGCCACGGGTAACGCCGCCCGTCTGTGCGCCGCC
GTGGGCTGTGACGAGGCGACTTTCCGCGCCGCCCATACTCTGATCCTGCGGCTTGAACCCCGCCCCGGTCGCGCCTGGAC
CGTGCCGGCCGCCGACTACGCCGTGCCCGACGTCATCGTGCGCAAGACCCGGCGCGGCTGGCAGGTCACGCTGAATAGCG
CTGCGGTGCCCCGGCTACAGATCAACGGCCTGTATGCGCAGATGCTGGGCAATCAGAAGGAATCCGCGCACGCCGGCTTG
CAGACCCAGTTGCAGCAGGCCAGATGGATGATCCGCAACGTGGAGCAGCGCTTTGACACGATCCTGCGGGTGGCCCAGGC
GATCGTGGAACGCCAGACTGCGTTCTTCAGTCAAGGGCCATCGGCCATGCGTCCGCTGATCCTGAAAGACATTGCGGGCG
AGCTTGGCTTGCACGAATCGACCATTTCACGGGCTACTACGCAGAAGTACATGCTTACTCCGTTTGGTACGCTGGAGCTC
AAGCGCTTCTTCGGCACGGGCGTGTCCACGGACGGGGGGGATGCGACTTCCGCCACCGCGGTGCAGACGTTCATCCGCCA
AATGGTGGCGGAAGAGAACCGCGCCAAGCCCTTGTCGGATAGCCAGATCATGCAGAAACTGGCCGATCAGGGGATAGTCA
TCGCCCGCCGGACGGTGGCAAAGTACCGCGAGGCGCTGCGGATTGCCCCCGCCACGCTGCGCAAGGCGCAGGCCAGCGCG
CGTTGA

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(12-55)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(108-305)

Sigma-54, DNA binding domain (Sigma54_DBD)

(320-475)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

48.907

100

0.511

Multiple sequence alignment