Detailed information    

insolico Bioinformatically predicted

Overview

Name   recO   Type   Machinery gene
Locus tag   EKQ63_RS23055 Genome accession   NZ_CP034686
Coordinates   4012059..4012805 (-) Length   248 a.a.
NCBI ID   WP_000487010.1    Uniprot ID   Q81LT9
Organism   Bacillus sp. BD59S     
Function   ssDNA annealing; plasmid transformation (predicted from homology)   
DNA processing Homologous recombination

Genomic Context

Location: 4007059..4017805
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EKQ63_RS23040 (EKQ63_23020) dnaG 4007826..4009622 (-) 1797 WP_048550869.1 DNA primase -
  EKQ63_RS23045 (EKQ63_23025) - 4010477..4011289 (-) 813 WP_000368943.1 pyruvate, water dikinase regulatory protein -
  EKQ63_RS23050 (EKQ63_23030) - 4011319..4011951 (-) 633 WP_000583759.1 helix-turn-helix transcriptional regulator -
  EKQ63_RS23055 (EKQ63_23035) recO 4012059..4012805 (-) 747 WP_000487010.1 DNA repair protein RecO Machinery gene
  EKQ63_RS23060 (EKQ63_23040) - 4012839..4012982 (-) 144 WP_000883924.1 YqzL family protein -
  EKQ63_RS23065 (EKQ63_23045) era 4013159..4014064 (-) 906 WP_001080241.1 GTPase Era -
  EKQ63_RS23070 (EKQ63_23050) - 4014057..4014455 (-) 399 WP_001086294.1 cytidine deaminase -
  EKQ63_RS23075 (EKQ63_23055) - 4014555..4014908 (-) 354 WP_000715476.1 diacylglycerol kinase family protein -
  EKQ63_RS23080 (EKQ63_23060) ybeY 4014905..4015375 (-) 471 WP_000054691.1 rRNA maturation RNase YbeY -
  EKQ63_RS23085 (EKQ63_23065) - 4015372..4017516 (-) 2145 WP_016718495.1 HD family phosphohydrolase -

Sequence

Protein


Download         Length: 248 a.a.        Molecular weight: 28665.22 Da        Isoelectric Point: 8.0540

>NTDB_id=332501 EKQ63_RS23055 WP_000487010.1 4012059..4012805(-) (recO) [Bacillus sp. BD59S]
MFQKVEGIVIRTTDYGETNKIVTIFSRELGKVSAMARGAKKPKSRLASVSQLMTHGHFLIQMGSGLGTLQQGEIISTMKE
IREDIFLTAYASFIVELTDKATEDKKHNPYLFEMLYQTLHYMCEGVDPEVLSLIYQTKMLPVLGMRPYFDTCAICHQETD
FVAFSVREGGFLCSRHAEQDQYRIPVGEAVHKLLRLFYHFDLHRLGNVSVKDSTKKQMRLVLNTYYDEYCGIYLKSRRFL
EQLDKFQI

Nucleotide


Download         Length: 747 bp        

>NTDB_id=332501 EKQ63_RS23055 WP_000487010.1 4012059..4012805(-) (recO) [Bacillus sp. BD59S]
ATGTTTCAAAAAGTTGAGGGCATCGTTATCCGTACGACAGATTACGGAGAAACGAACAAGATTGTTACAATATTCTCAAG
AGAATTGGGTAAGGTAAGTGCAATGGCAAGAGGAGCGAAAAAACCGAAAAGCCGGTTAGCATCTGTTTCGCAACTTATGA
CACATGGTCATTTTCTTATTCAAATGGGATCTGGACTTGGAACTTTGCAACAAGGCGAGATTATTTCAACTATGAAAGAA
ATTCGCGAGGATATATTTTTAACTGCTTATGCATCATTTATTGTTGAGTTAACTGATAAAGCAACAGAAGATAAAAAACA
TAATCCATATTTATTTGAAATGTTATATCAAACGTTGCATTATATGTGTGAGGGTGTTGATCCAGAAGTATTATCATTAA
TTTATCAAACGAAAATGCTTCCGGTATTAGGGATGCGCCCGTACTTTGATACATGTGCGATTTGTCATCAAGAAACAGAT
TTTGTCGCCTTCTCTGTTAGGGAGGGCGGTTTTCTGTGCTCACGTCATGCTGAGCAAGATCAGTATCGTATACCAGTGGG
GGAAGCCGTTCATAAATTATTGCGTCTTTTCTACCACTTCGATTTACATAGACTGGGCAATGTATCAGTGAAGGATAGCA
CAAAAAAACAAATGCGTTTAGTATTGAATACATATTATGATGAATATTGCGGGATTTATTTGAAATCAAGACGTTTTTTA
GAACAACTTGATAAGTTTCAAATATAA

Domains

Predicted by InterproScan.

Recombination protein O C terminal (RecO_C)

(82-238)

Recombination protein O N terminal (RecO_N)

(1-77)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q81LT9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recO Bacillus subtilis subsp. subtilis str. 168

55.466

99.597

0.552

Multiple sequence alignment