Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   EKQ63_RS22780 Genome accession   NZ_CP034686
Coordinates   3958067..3959110 (-) Length   347 a.a.
NCBI ID   WP_087954298.1    Uniprot ID   -
Organism   Bacillus sp. BD59S     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3953067..3964110
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EKQ63_RS22740 (EKQ63_22720) - 3953272..3953616 (+) 345 WP_143881532.1 helix-turn-helix domain-containing protein -
  EKQ63_RS22745 (EKQ63_22725) - 3953809..3954177 (+) 369 WP_143881533.1 hypothetical protein -
  EKQ63_RS30520 - 3954220..3954345 (-) 126 WP_263053821.1 hypothetical protein -
  EKQ63_RS22750 (EKQ63_22730) - 3954515..3955684 (+) 1170 WP_143881534.1 site-specific integrase -
  EKQ63_RS30525 (EKQ63_22735) - 3955827..3956015 (-) 189 Protein_4085 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  EKQ63_RS22760 (EKQ63_22740) comGE 3955985..3956287 (-) 303 WP_143881781.1 competence type IV pilus minor pilin ComGE -
  EKQ63_RS22765 (EKQ63_22745) comGD 3956280..3956735 (-) 456 WP_087954300.1 comG operon protein ComGD -
  EKQ63_RS22770 (EKQ63_22750) comGC 3956732..3957031 (-) 300 WP_001178694.1 competence type IV pilus major pilin ComGC -
  EKQ63_RS22775 (EKQ63_22755) comGB 3957043..3958074 (-) 1032 WP_088326717.1 comG operon protein ComGB -
  EKQ63_RS22780 (EKQ63_22760) comGA 3958067..3959110 (-) 1044 WP_087954298.1 competence protein ComGA Machinery gene
  EKQ63_RS22785 (EKQ63_22765) - 3959316..3960011 (+) 696 WP_000434105.1 helix-turn-helix transcriptional regulator -
  EKQ63_RS22790 (EKQ63_22770) - 3960137..3960379 (+) 243 WP_000440714.1 DUF2626 domain-containing protein -
  EKQ63_RS22795 (EKQ63_22775) - 3960486..3961880 (+) 1395 WP_001094339.1 L-cystine transporter -
  EKQ63_RS22800 (EKQ63_22780) - 3962055..3962462 (-) 408 WP_088326719.1 hypothetical protein -
  EKQ63_RS22805 (EKQ63_22785) - 3962550..3962765 (-) 216 WP_001008331.1 DUF3912 family protein -
  EKQ63_RS22810 (EKQ63_22790) - 3963019..3963330 (+) 312 WP_029439923.1 hypothetical protein -
  EKQ63_RS22815 (EKQ63_22795) - 3963366..3963854 (-) 489 WP_140421179.1 hypothetical protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 39345.84 Da        Isoelectric Point: 9.1826

>NTDB_id=332500 EKQ63_RS22780 WP_087954298.1 3958067..3959110(-) (comGA) [Bacillus sp. BD59S]
MNGIESFANMILKEACRVQASDLHIVPRKKDVAVQLRIGKDLMTKQYIEKEFGEKLVSHFKFLASMDIGERRKPQNGSLY
LQIDGQEVYLRLSTLPTVYQESLVIRLHLQASIQPLSHLSLFPSTAKKLLSFLHYSHGLLVFTGPTGSGKTTTMYALLEV
IRKKKTRRIVTLEDPVEKRNDDVLQIQINEKAGITYEAGLKAILRHDPDIILVGEIRDEETAKIAIRASLTGHLVMTTLH
TNDAKGAILRFMDFGITRQEIEQSLLAIAAQRLVELKCPFCKRKCSTVCKSMRQVRQASIYELLYGYELKQAIKEANGEC
VTYKHETLESSLRKGYALGFLEEDVYV

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=332500 EKQ63_RS22780 WP_087954298.1 3958067..3959110(-) (comGA) [Bacillus sp. BD59S]
ATGAATGGAATTGAAAGCTTTGCGAATATGATTTTGAAAGAGGCGTGTAGGGTACAAGCTTCGGATTTACATATTGTGCC
CCGGAAGAAGGATGTAGCGGTTCAACTGCGTATAGGAAAAGATTTAATGACGAAACAATACATTGAAAAGGAATTTGGAG
AAAAACTTGTTTCACACTTTAAATTTTTAGCATCCATGGATATAGGAGAGAGGCGGAAACCACAAAACGGTTCACTGTAT
TTACAAATAGATGGACAAGAAGTGTATTTACGCCTTTCCACGCTTCCAACCGTATATCAAGAAAGTCTCGTTATTCGTCT
TCATTTACAAGCATCTATTCAGCCGTTATCTCATCTTTCGTTATTTCCAAGTACAGCGAAGAAACTACTTTCTTTTTTAC
ATTATTCTCATGGATTACTCGTCTTTACGGGACCGACTGGTTCAGGAAAGACAACAACAATGTATGCATTATTAGAAGTA
ATTAGAAAAAAGAAAACACGTCGCATCGTTACGCTGGAAGATCCAGTTGAAAAAAGAAATGACGATGTATTACAAATTCA
AATAAATGAAAAAGCAGGTATAACATATGAGGCGGGACTGAAGGCTATTTTACGTCATGATCCAGATATTATTTTAGTCG
GTGAAATTCGTGATGAAGAAACAGCAAAAATCGCTATAAGAGCAAGTTTGACTGGCCATTTAGTAATGACGACACTGCAT
ACGAATGATGCGAAAGGAGCAATACTTCGGTTTATGGACTTTGGCATAACAAGGCAAGAGATTGAACAATCTTTATTGGC
TATAGCTGCACAGCGACTTGTTGAATTGAAGTGTCCGTTTTGCAAAAGAAAGTGCTCAACTGTATGTAAATCAATGAGGC
AAGTAAGGCAAGCGAGTATTTATGAGTTGTTATATGGGTATGAATTAAAACAAGCGATTAAAGAAGCAAACGGGGAATGT
GTCACGTACAAGCATGAGACATTAGAGTCTTCGCTGCGAAAAGGGTACGCTTTAGGATTTTTAGAAGAGGATGTTTATGT
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

58.213

100

0.582
  pilB Vibrio campbellii strain DS40M4

35.977

100

0.366

Multiple sequence alignment