Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   EKQ63_RS21285 Genome accession   NZ_CP034686
Coordinates   3713208..3713642 (-) Length   144 a.a.
NCBI ID   WP_087954431.1    Uniprot ID   -
Organism   Bacillus sp. BD59S     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3708208..3718642
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EKQ63_RS21270 (EKQ63_21250) - 3709231..3709821 (+) 591 WP_087954433.1 PadR family transcriptional regulator -
  EKQ63_RS21275 (EKQ63_21255) metE 3710457..3712745 (+) 2289 WP_087954432.1 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase -
  EKQ63_RS21280 (EKQ63_21260) comJ 3712783..3713172 (-) 390 WP_000424015.1 competence protein ComJ -
  EKQ63_RS21285 (EKQ63_21265) nucA/comI 3713208..3713642 (-) 435 WP_087954431.1 DNA-entry nuclease Machinery gene
  EKQ63_RS21290 (EKQ63_21270) - 3713879..3714628 (+) 750 WP_087954430.1 DUF3967 domain-containing protein -
  EKQ63_RS21295 (EKQ63_21275) kinB 3714682..3715956 (-) 1275 WP_087954429.1 sporulation sensor histidine kinase KinB -
  EKQ63_RS21300 (EKQ63_21280) - 3716266..3716466 (+) 201 WP_000929285.1 hypothetical protein -
  EKQ63_RS21305 (EKQ63_21285) - 3716590..3717753 (+) 1164 WP_048550590.1 aminotransferase A -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16010.38 Da        Isoelectric Point: 8.4331

>NTDB_id=332496 EKQ63_RS21285 WP_087954431.1 3713208..3713642(-) (nucA/comI) [Bacillus sp. BD59S]
MKQLKGIIISIIAILSILVAVYEVLVPEETSIKKTNAYDQVLEFPKDRYPETGKHITDAIKEGHSEVCTIDRGGAADRRK
LSLAPYPSKKGYDRDEWPMAMCKEGGKGAHIEYISPADNRGAGSWVGNKLDKYPDGTRVKFDVK

Nucleotide


Download         Length: 435 bp        

>NTDB_id=332496 EKQ63_RS21285 WP_087954431.1 3713208..3713642(-) (nucA/comI) [Bacillus sp. BD59S]
ATGAAACAATTAAAAGGTATTATCATTTCAATTATTGCAATTCTTTCTATTCTAGTAGCGGTGTATGAAGTGCTTGTTCC
AGAGGAAACAAGTATTAAAAAAACAAATGCGTATGATCAAGTTCTAGAATTTCCGAAAGATCGATATCCAGAGACAGGGA
AACATATTACGGATGCTATAAAAGAAGGACATTCAGAAGTTTGTACAATCGACCGTGGTGGCGCTGCGGATAGAAGAAAA
TTATCGTTAGCTCCATACCCATCAAAAAAAGGGTATGATCGTGATGAATGGCCAATGGCGATGTGTAAAGAAGGTGGAAA
AGGAGCACATATTGAATATATAAGTCCAGCCGATAACCGCGGAGCAGGCTCTTGGGTAGGGAATAAGTTAGATAAATACC
CAGATGGTACGCGTGTGAAATTTGACGTAAAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

72.072

77.083

0.556

Multiple sequence alignment