Detailed information NTG0332389

Bioinformatically predicted

Overview

• Name: chiS
• Type: Regulator
• Locus tag: DYL72_RS10655
• Genome accession: NZ_CP034672
• Coordinates: 2227386..2230778 (-)
• Length: 1130 a.a.
• NCBI ID: WP_029388441.1
• Uniprot ID: A0A289GER5
• Organism: Vibrio anguillarum strain J360
• Function: chitin sensor (predicted from homology) Competence regulation

Genomic Context

Location: 2222386..2235778

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
DYL72_RS10640 (DYL72_10640)	-	2222790..2223815 (-)	1026	WP_013857641.1	ABC transporter permease	-
DYL72_RS10645 (DYL72_10645)	-	2223818..2224804 (-)	987	WP_013857640.1	ABC transporter permease	-
DYL72_RS10650 (DYL72_10650)	-	2224971..2226641 (-)	1671	WP_069212218.1	ABC transporter substrate-binding protein	-
DYL72_RS10655 (DYL72_10655)	chiS	2227386..2230778 (-)	3393	WP_029388441.1	response regulator	Regulator
DYL72_RS10660 (DYL72_10660)	rsmC	2230978..2232021 (-)	1044	WP_019283245.1	16S rRNA (guanine(1207)-N(2))-methyltransferase RsmC	-
DYL72_RS10665 (DYL72_10665)	-	2232105..2233190 (-)	1086	WP_017045856.1	AI-2E family transporter	-
DYL72_RS10670 (DYL72_10670)	hemL	2233468..2234763 (-)	1296	WP_029388440.1	glutamate-1-semialdehyde 2,1-aminomutase	-
DYL72_RS10675 (DYL72_10675)	erpA	2235086..2235427 (+)	342	WP_026027518.1	iron-sulfur cluster insertion protein ErpA	-

Sequence

Protein

• Length: 1130 a.a.
• Molecular weight: 127839.55 Da
• Isoelectric Point: 6.2690

>NTDB_id=332389 DYL72_RS10655 WP_029388441.1 2227386..2230778(-) (chiS) [Vibrio anguillarum strain J360]
MFRFSRKQKFKRLQNTLMLAFLVLSITPLTVTAIFFLQSHTKDLQEQSTSHLLSVRDTKQQQVLDYLTAKESEVMGFVRS
ELAYASGGRFYGLVNAFQRLGLDIDQAREHAQQRYIPGSGDQIKTSILPQSSSYVGSERYRLLHKRYHWAYLELLKRSDF
DDILLVDLDGDVVYSVYKHDNYGTNLLTGKYKDSNLGKTFRQLEQQVGEQRKINEEYTPVIISDFSQQEGQSVAWLGAPI
IQQGYLHSYAMFRLPNNGITKLVADTNATSQIRTLLVGQDHHSRILSYTQQEIETSQKVINLALSGLSAVGTYTNTLNEK
TIAAYAPVANKNINWALVVEILEKEAYARVHQLEKLFIFAMLTAIILVVIASHYLSNFITSPLLKLTWAAEKVSAGDLDE
NVINTDRKDEIGRLAVSFERMQRSIREKMQLIKAQNKELESNIQLIRKQNDELHLANKLKDEFLATTSHELRTPLHGMVG
IAEALISGSSGTITADHRYQLGIIISSGQRLTNLVDDLLDYHKMRYGSLDIKQCAVDLSGATRLVLELSNHLLNKKPIRV
INQIPDECIWVSADPQRLEQVLYNLVGNAIKYTTEGKIVISANVVDDKIRVQVVDTGQGIPAEQLEHIFEPLIQAGQDAS
RYRQGAGLGLSISRQLIELMQGTLYVSSQPMVGTTFSFTLPLASKAEIESTSYMEAGHFQSPTISDIELPEMHSLPENKE
GPVLLIADDEPVNLRVLDSFLRLEGYQVRTAKDGNEVISAVEKEKPELLLLDIMMPGLSGYQVCEKLRETYDHAELPIIM
LTALNQTDDRVRGFAAGANDYLSKPFNKQELAARITAHLTASKAEQRRIENNQLQQELKHRAMVEASLLETQGRLLEQLE
SAPEAIICIRDDHKIRFANEAAARLFKRSQEQLKRSNAEELIAPKYLVVEQEHYCGNIDIYVEDTRQHMSADVLRLPDGS
GLQAMYIFNVGGNINAARINNLETAIEALSSYAFEGDKEKLQQLKELGGEFTRIADKVSGESQSKQDLMREVLVEAMTSA
LDYWERVSNQSKFTFAEQSGLWRVYLDRSTLQTRTLDKYLRIETLPKTPRWRTVLNSLDYILDHCQEHGPERSHIEAQRD
KLQKLLTSSL

Nucleotide

• Length: 3393 bp

>NTDB_id=332389 DYL72_RS10655 WP_029388441.1 2227386..2230778(-) (chiS) [Vibrio anguillarum strain J360]
ATGTTTAGGTTCTCTCGTAAGCAAAAATTTAAGCGCCTGCAAAATACTCTTATGCTGGCTTTTCTCGTTTTGAGCATCAC
GCCTTTAACTGTGACTGCCATTTTTTTTCTGCAATCACACACTAAAGATCTTCAAGAACAAAGCACGTCTCACTTATTGT
CAGTCAGAGACACTAAGCAACAGCAAGTTCTCGATTACCTCACGGCCAAAGAATCAGAAGTGATGGGGTTTGTCCGTTCA
GAGTTAGCTTATGCTAGTGGAGGGCGCTTTTACGGCTTAGTCAATGCGTTCCAAAGACTTGGTCTGGATATTGATCAAGC
TCGTGAGCATGCCCAGCAGCGTTATATTCCTGGCTCTGGTGATCAGATAAAAACGTCAATATTGCCACAATCGAGCAGCT
ATGTAGGCAGTGAACGGTATCGCCTACTGCACAAACGCTATCATTGGGCGTATTTAGAACTGCTTAAACGTTCTGATTTT
GACGATATTTTACTGGTGGATCTCGATGGTGATGTCGTTTATTCCGTTTATAAGCACGACAACTATGGGACGAATTTACT
GACCGGAAAATACAAAGATAGCAATCTGGGTAAAACATTCCGCCAGTTAGAGCAGCAAGTTGGCGAACAACGAAAAATCA
ATGAGGAATATACACCAGTCATTATTTCTGATTTTTCACAACAAGAAGGGCAATCAGTTGCTTGGCTTGGCGCGCCCATT
ATTCAACAGGGCTACCTGCACAGCTACGCCATGTTCCGCCTACCAAATAATGGCATCACAAAGCTCGTTGCTGACACCAA
TGCGACATCGCAAATAAGAACTTTACTCGTCGGGCAAGATCATCATTCTCGAATACTCTCTTACACGCAGCAAGAAATCG
AGACTAGTCAAAAAGTGATCAATCTTGCGCTTTCTGGATTGAGTGCCGTTGGTACTTACACTAATACATTGAATGAGAAA
ACCATTGCCGCCTATGCACCTGTTGCAAACAAAAACATCAATTGGGCCTTGGTCGTAGAGATACTAGAAAAAGAGGCTTA
TGCGCGAGTTCATCAGCTTGAGAAGTTGTTTATTTTCGCCATGCTAACCGCGATTATTTTAGTCGTCATCGCTTCACATT
ATCTCTCTAATTTTATTACCTCTCCCCTACTGAAGCTCACTTGGGCGGCAGAAAAAGTCTCAGCTGGGGATTTAGATGAA
AACGTTATCAATACCGATCGTAAAGATGAGATTGGTCGGCTCGCAGTGAGTTTTGAACGTATGCAGCGTTCGATTCGAGA
AAAAATGCAGCTGATTAAAGCGCAAAATAAAGAGCTCGAAAGCAACATTCAATTAATCCGCAAACAGAACGATGAGTTGC
ATCTCGCTAACAAACTAAAAGATGAATTCCTAGCCACAACCTCACATGAATTAAGAACACCGTTGCACGGTATGGTCGGT
ATTGCAGAAGCGCTGATCTCTGGGTCAAGTGGCACGATCACCGCCGATCACCGTTATCAATTGGGCATAATCATCAGCAG
TGGACAACGGCTGACAAACTTGGTCGATGACTTACTCGATTATCATAAGATGCGCTATGGCAGCTTGGATATCAAACAGT
GTGCTGTTGATCTATCCGGTGCAACACGCTTAGTGTTAGAGCTGTCTAACCATCTACTCAACAAAAAGCCCATTCGCGTA
ATAAACCAAATCCCTGATGAATGCATTTGGGTCTCTGCCGATCCACAACGATTAGAGCAAGTGCTTTATAACCTTGTAGG
AAACGCCATTAAATATACGACTGAAGGGAAAATTGTCATTTCAGCCAATGTGGTTGATGACAAGATTCGTGTCCAAGTTG
TCGATACGGGGCAAGGCATTCCTGCTGAACAACTTGAACACATCTTCGAACCACTGATTCAAGCAGGGCAAGATGCCAGT
CGTTATCGACAAGGCGCAGGCTTAGGTCTTTCGATCAGCCGTCAATTGATCGAATTAATGCAAGGAACGCTTTATGTCAG
TAGCCAACCTATGGTCGGTACGACATTCAGTTTTACCTTACCATTAGCATCAAAAGCGGAAATTGAATCGACAAGCTATA
TGGAAGCTGGCCATTTCCAATCTCCGACCATTTCAGACATTGAACTGCCTGAAATGCATTCACTACCTGAAAATAAAGAA
GGCCCAGTGCTACTGATAGCAGATGATGAACCCGTCAATCTGCGTGTCCTAGACAGCTTCCTACGTTTAGAAGGCTATCA
AGTCCGCACCGCTAAAGATGGCAATGAAGTCATTAGCGCGGTCGAAAAAGAAAAACCGGAACTTCTACTGCTCGACATTA
TGATGCCAGGATTAAGCGGCTACCAAGTCTGTGAAAAGTTAAGAGAAACATACGATCATGCCGAGCTACCTATCATTATG
TTGACGGCATTAAATCAAACTGACGATAGAGTTCGTGGTTTTGCAGCGGGAGCGAACGATTACTTATCGAAGCCGTTTAA
TAAGCAAGAGCTCGCTGCACGTATTACGGCGCATTTAACGGCCAGTAAAGCTGAACAAAGACGTATTGAGAACAACCAAC
TGCAACAAGAGCTGAAGCATAGAGCAATGGTAGAGGCCAGTTTATTGGAAACCCAAGGCCGTCTACTAGAGCAGCTCGAA
TCCGCTCCTGAGGCCATTATTTGCATTCGAGACGATCATAAAATCCGCTTTGCGAACGAAGCGGCTGCTCGGTTATTTAA
GCGCAGCCAAGAACAACTAAAGCGCTCAAATGCGGAAGAACTCATCGCCCCCAAATACTTAGTCGTCGAGCAAGAGCATT
ACTGCGGTAATATCGATATCTATGTCGAAGATACACGTCAGCATATGTCTGCTGATGTGTTACGACTACCGGATGGCTCT
GGGTTGCAGGCTATGTACATCTTTAACGTGGGTGGAAACATCAATGCAGCGCGCATCAATAACCTTGAAACCGCTATTGA
AGCGCTATCCAGTTACGCATTTGAAGGCGATAAAGAAAAACTGCAACAGCTCAAAGAACTCGGTGGCGAGTTCACACGTA
TCGCCGACAAAGTATCGGGAGAGAGTCAATCTAAGCAAGATTTAATGCGCGAAGTACTGGTTGAAGCAATGACCAGTGCT
TTAGATTACTGGGAACGGGTCAGTAACCAAAGCAAGTTTACCTTTGCAGAGCAGAGTGGGCTATGGCGAGTATATCTCGA
TAGAAGCACATTACAGACTCGGACACTAGACAAGTACTTACGTATCGAGACACTGCCTAAAACACCTCGCTGGCGTACCG
TTCTTAATTCGTTAGATTACATTCTTGATCATTGTCAGGAGCACGGCCCTGAACGCTCTCACATCGAAGCGCAGCGCGAT
AAATTGCAAAAGCTACTGACCTCATCACTCTAA

Domains

Predicted by InterproScan.

• HAMP domain (HAMP) (375-427)

• Response regulator receiver domain (Response_reg) (725-835)

• His Kinase A (phospho-acceptor) domain (HisKA) (460-525)

• PAS fold (PAS) (876-926)

• Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (HATPase_c) (574-683)

3D structure

• AlphaFold DB: A0A289GER5

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
chiS	Vibrio cholerae strain A1552	81.56	99.823	0.814