Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   DYL72_RS05820 Genome accession   NZ_CP034672
Coordinates   1242427..1243437 (-) Length   336 a.a.
NCBI ID   WP_019282438.1    Uniprot ID   A0A289GF81
Organism   Vibrio anguillarum strain J360     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1237427..1248437
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DYL72_RS05795 (DYL72_05795) aroK 1238294..1238815 (-) 522 WP_010317691.1 shikimate kinase AroK -
  DYL72_RS05800 (DYL72_05800) pilQ 1239002..1240687 (-) 1686 WP_370685857.1 type IV pilus secretin PilQ Machinery gene
  DYL72_RS05805 (DYL72_05805) pilP 1240741..1241262 (-) 522 WP_013855888.1 pilus assembly protein PilP Machinery gene
  DYL72_RS05810 (DYL72_05810) pilO 1241249..1241842 (-) 594 WP_013855887.1 type 4a pilus biogenesis protein PilO Machinery gene
  DYL72_RS05815 (DYL72_05815) pilN 1241835..1242437 (-) 603 WP_017047271.1 PilN domain-containing protein Machinery gene
  DYL72_RS05820 (DYL72_05820) pilM 1242427..1243437 (-) 1011 WP_019282438.1 type IV pilus assembly protein PilM Machinery gene
  DYL72_RS05825 (DYL72_05825) - 1243587..1246085 (+) 2499 WP_019282439.1 penicillin-binding protein 1A -
  DYL72_RS05830 (DYL72_05830) oxyR 1246122..1247012 (-) 891 WP_026027425.1 DNA-binding transcriptional regulator OxyR -
  DYL72_RS05835 (DYL72_05835) - 1247159..1247887 (+) 729 WP_013855882.1 redoxin family protein -

Sequence

Protein


Download         Length: 336 a.a.        Molecular weight: 37427.96 Da        Isoelectric Point: 7.5395

>NTDB_id=332368 DYL72_RS05820 WP_019282438.1 1242427..1243437(-) (pilM) [Vibrio anguillarum strain J360]
MGKSLVTGIDIGHHSIKAVVLKLAGDSYALVGYKELFITEDIFSDNHTLDYQKIVKKLKELRKGLPLFSRKVAISVPDNA
VISKVLQIDSDLQAREKEFAIYQAFSHQSPFPIEELSLDFVKVMEKSVSRSSTVSYQVYATRKDVVESRALAAQKAGFLP
IAVDVQIHSLLSIWQLASRAQHRSDWMLMDVGHTQTALCIDFTDKAPFHKEIPLGCQWLSQDSAMSGTNAELADKNERFV
HELIERLQRQLQLFTSVNGAGSLAGIWLSGGGADTPMLAQEISRRLKLECEILDPFLLFNNKLAKRQRDKQSGQSFSTAA
GLALRGIEWLESSHVA

Nucleotide


Download         Length: 1011 bp        

>NTDB_id=332368 DYL72_RS05820 WP_019282438.1 1242427..1243437(-) (pilM) [Vibrio anguillarum strain J360]
ATGGGTAAATCATTGGTTACGGGTATAGATATTGGCCACCATAGTATTAAAGCGGTGGTGTTAAAGCTAGCGGGTGATTC
ATATGCACTCGTCGGTTATAAAGAATTATTTATTACTGAGGACATATTCTCCGATAACCACACGCTGGATTATCAGAAAA
TTGTAAAGAAACTCAAAGAACTAAGAAAGGGTTTACCACTATTTAGTCGCAAAGTCGCCATATCGGTACCGGACAATGCG
GTAATAAGTAAGGTATTACAGATAGATAGTGACCTGCAGGCGCGTGAAAAAGAGTTTGCTATTTATCAGGCATTTTCTCA
TCAGTCACCGTTCCCGATAGAAGAACTGAGTCTCGATTTCGTCAAAGTGATGGAAAAAAGCGTAAGTCGCAGTAGCACCG
TAAGTTATCAAGTTTACGCCACTCGTAAAGACGTCGTGGAAAGCCGTGCGTTAGCGGCTCAAAAAGCGGGCTTTTTACCG
ATCGCGGTTGATGTGCAAATACACAGTTTGTTAAGCATCTGGCAGTTGGCGAGCAGGGCACAACATCGCAGCGATTGGAT
GCTCATGGATGTGGGCCATACCCAAACGGCGCTCTGTATTGATTTTACTGATAAAGCCCCTTTTCATAAAGAGATCCCTC
TCGGTTGTCAGTGGTTGTCCCAAGACAGCGCGATGTCGGGCACCAATGCGGAATTAGCCGATAAAAACGAGCGCTTTGTG
CATGAGTTGATCGAGCGACTTCAACGTCAGTTGCAGCTTTTCACCTCCGTCAATGGCGCGGGCAGTTTAGCAGGCATTTG
GCTCTCAGGTGGTGGCGCAGATACTCCGATGTTGGCGCAAGAGATCTCGCGGCGTCTCAAGCTTGAGTGCGAGATTCTCG
ATCCCTTCCTTCTGTTTAACAATAAGTTAGCGAAACGCCAGCGAGATAAGCAGTCTGGGCAGAGTTTTAGCACCGCGGCT
GGCCTTGCACTGCGTGGTATCGAGTGGTTGGAGTCTTCTCATGTTGCATAG

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A289GF81

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio cholerae strain A1552

67.456

100

0.679
  pilM Vibrio campbellii strain DS40M4

52.976

100

0.53

Multiple sequence alignment