Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   FVE22_RS03830 Genome accession   NZ_CP042844
Coordinates   819322..819762 (-) Length   146 a.a.
NCBI ID   WP_000360904.1    Uniprot ID   P36647
Organism   Escherichia coli strain JME66     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 814322..824762
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FVE22_RS03810 coaE 814812..815432 (-) 621 WP_001269520.1 dephospho-CoA kinase -
  FVE22_RS22580 - 815457..815501 (+) 45 WP_120795372.1 protein YacM -
  FVE22_RS03815 guaC 815657..816700 (+) 1044 WP_001217338.1 GMP reductase -
  FVE22_RS03820 hofC 816735..817937 (-) 1203 WP_000157266.1 protein transport protein HofC -
  FVE22_RS03825 pilB 817927..819312 (-) 1386 WP_001025145.1 type II secretion system protein GspE Machinery gene
  FVE22_RS03830 pilA 819322..819762 (-) 441 WP_000360904.1 prepilin peptidase-dependent pilin Machinery gene
  FVE22_RS03835 nadC 819965..820858 (-) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  FVE22_RS03840 ampD 820946..821497 (+) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  FVE22_RS03845 ampE 821494..822348 (+) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  FVE22_RS03850 aroP 822391..823764 (-) 1374 WP_000969915.1 aromatic amino acid transporter AroP -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15621.75 Da        Isoelectric Point: 4.3938

>NTDB_id=332057 FVE22_RS03830 WP_000360904.1 819322..819762(-) (pilA) [Escherichia coli strain JME66]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=332057 FVE22_RS03830 WP_000360904.1 819322..819762(-) (pilA) [Escherichia coli strain JME66]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P36647

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363