Detailed information    

insolico Bioinformatically predicted

Overview

Name   addA   Type   Machinery gene
Locus tag   EJ992_RS05885 Genome accession   NZ_CP034569
Coordinates   1180702..1184394 (+) Length   1230 a.a.
NCBI ID   WP_011197743.1    Uniprot ID   Q65LJ9
Organism   Bacillus licheniformis strain ATCC 14580     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1175702..1189394
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EJ992_RS05875 (EJ992_05875) modB 1176449..1177105 (+) 657 WP_009328900.1 molybdate ABC transporter permease subunit -
  EJ992_RS05880 (EJ992_05880) addB 1177215..1180715 (+) 3501 WP_009328899.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  EJ992_RS05885 (EJ992_05885) addA 1180702..1184394 (+) 3693 WP_011197743.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  EJ992_RS05890 (EJ992_05890) sbcD 1184459..1185640 (+) 1182 WP_011197744.1 exonuclease subunit SbcD -
  EJ992_RS05895 (EJ992_05895) sbcC 1185637..1189029 (+) 3393 WP_011197745.1 exonuclease subunit SbcC -
  EJ992_RS05900 (EJ992_05900) - 1189073..1189291 (-) 219 WP_009328895.1 spore germination protein -

Sequence

Protein


Download         Length: 1230 a.a.        Molecular weight: 141090.95 Da        Isoelectric Point: 5.5198

>NTDB_id=331975 EJ992_RS05885 WP_011197743.1 1180702..1184394(+) (addA) [Bacillus licheniformis strain ATCC 14580]
MEISKPKGSTWTDDQWKAIVSSGRDILVAAAAGSGKTAVLVERIIRKITDQERPVDVDRLLVVTFTNASAAEMKHRIGEA
LEKELAENPGSLHLRRQLALLNKASISTLHSFCLQVIRKYYYLIDVDPAFRIADQTEGELLGDEVLDELFEEEYKKGNPA
FFELVDRYTTDRHDLDLQHLVKRVYEFSRSHPDPEGWLHSLAELYDAASDTKVEALPFYSYIKEDIALVLEGMRQKLTRA
LDLTKQPGGPAPRAENFLDDLAQIDRLIQQQDDFSALHELVPTVSFQRLKPCKGDEYDPRLVDEAADLRNSAKKQLEKLK
SDYFSRTPEQHLESLREMKPVIQTLVQLVLEYGRRFAEAKKEKAIVDFSDLEHDCLAILSVKNPAGEAVPSEAAKFYRHQ
FHEVLVDEYQDTNLVQEAILKLVAKEEHEGNLFMVGDVKQSIYRFRLAEPLLFLSKYKRFTDDGSGSGQKIDLNKNFRSR
SDILDSTNFLFKQLMGEKVGEVEYDEQAELKLGASYPPNEATKTELLLIETPSGAPGEEAEELEAVQLEARAMAGQIRRL
ITEKFQVYDAKAKASRNIQYRDIVVLLRSMPWAPQIMDEFKQQGIPVYANLSTGYFEATEVSVTLSLLKIIDNAYQDIPL
ASVLRSPVVGLDENELSLIRIKDKKAPFYEAMKAYLAAADGDERLSEKLRRFDGLLKKWRAYAKNHSVAELIWEIYRDTK
YLDYVGGMPGGKQRQANLRALYDRARSYEATSFRGLFRFLRFIERMQERGDDLGTARALSEQEDVVRLMTIHSSKGLEFP
VVFTAGLGRNFNMMDLNKSYLLDKELGFGTKFIHPKWRISYPTLPLIAMKKKLRRELLSEELRVLYVALTRAKEKLYLVG
TAKDKEKLLADWRTQAAGSEWLLPDYERFQAKSYLDFIGPALMRHRDMDESGAPPVIDEIREHPARFQVSWLSAADLQAE
AVDQAGEERHDRLVQIQMGHAIEGAFEYEQQVRERLAWSYPYKDAAKVRTKQSVSEMKRQKEYEDEYGDRSLIRPSQEAL
LFKRPSFMMAKGLTAAERGTAMHTVMQHIPLTRTPEKNELSRLLDRLVEKELLTEDQRAAIEEDDILAFFDTEIGQKLFG
ARRVEREVPFNMTLSAKEVYPDLESADEPVLIQGIIDCLFETEDGFYLLDYKTDRIHGKYRNGFEGAEPILRKRYETQIQ
LYARAVETMIKMPLKGRALYFFDGGHVLLF

Nucleotide


Download         Length: 3693 bp        

>NTDB_id=331975 EJ992_RS05885 WP_011197743.1 1180702..1184394(+) (addA) [Bacillus licheniformis strain ATCC 14580]
ATGGAGATTTCTAAACCGAAGGGCAGCACCTGGACTGATGACCAATGGAAGGCGATTGTCTCATCCGGCCGGGACATTTT
AGTTGCGGCAGCGGCCGGTTCCGGAAAAACGGCCGTGCTGGTTGAACGGATCATTCGGAAAATAACGGATCAGGAACGAC
CGGTAGATGTCGACAGGCTGCTCGTCGTCACCTTTACAAATGCATCTGCGGCTGAAATGAAGCACAGGATCGGCGAAGCG
CTCGAAAAAGAGCTTGCCGAAAATCCGGGATCGCTTCATTTGCGCCGACAGCTTGCTTTATTAAACAAAGCGAGCATATC
AACATTGCATTCATTCTGTCTTCAAGTCATCCGCAAATATTACTATTTGATCGATGTCGATCCGGCTTTCCGGATCGCCG
ATCAGACGGAAGGCGAGCTGCTCGGCGATGAGGTGCTGGATGAACTGTTTGAAGAAGAATACAAAAAAGGCAATCCTGCC
TTTTTTGAACTTGTAGACAGATATACGACAGACCGTCATGACCTCGACCTGCAGCATCTTGTCAAGCGGGTCTACGAATT
TTCCCGTTCGCATCCCGATCCGGAGGGCTGGCTTCACTCATTGGCTGAACTGTATGACGCGGCTTCGGATACGAAGGTTG
AGGCTCTGCCGTTTTATTCTTACATTAAAGAAGACATCGCACTCGTTCTGGAAGGTATGCGGCAAAAGCTGACGCGCGCG
CTTGATTTGACGAAGCAGCCCGGGGGGCCTGCTCCGCGCGCCGAAAATTTTCTCGATGATTTAGCGCAGATCGACAGGCT
GATTCAGCAGCAGGATGATTTTTCGGCCCTGCATGAGCTTGTACCGACCGTTTCGTTTCAACGATTGAAGCCATGCAAAG
GTGATGAGTACGATCCCAGATTGGTTGATGAGGCGGCAGACCTCCGCAACAGTGCGAAAAAGCAGCTTGAAAAGCTGAAA
AGCGATTATTTTTCGCGAACTCCGGAGCAGCACCTTGAGAGCCTGAGGGAAATGAAGCCTGTCATTCAGACGCTTGTCCA
GCTCGTGCTGGAGTATGGCAGGCGGTTTGCGGAAGCGAAAAAGGAAAAGGCGATCGTCGATTTTTCAGACCTTGAACACG
ACTGCCTTGCGATTTTATCCGTGAAAAATCCGGCGGGCGAGGCCGTGCCGAGCGAAGCGGCGAAGTTTTACCGCCATCAG
TTCCACGAAGTGCTTGTTGATGAATACCAGGATACGAACCTGGTCCAGGAAGCGATTTTAAAGCTCGTCGCCAAAGAGGA
GCATGAGGGGAACCTGTTTATGGTGGGCGATGTCAAGCAGTCGATTTACCGGTTCCGCCTGGCTGAGCCGCTTTTGTTCC
TCTCGAAATATAAACGCTTTACAGACGATGGCTCGGGTTCCGGCCAGAAAATCGATTTAAATAAAAACTTCAGAAGCCGT
TCAGATATTTTGGACAGCACGAACTTTTTATTCAAACAGCTCATGGGAGAAAAAGTCGGGGAAGTCGAGTATGACGAACA
AGCCGAATTGAAGCTCGGCGCCTCTTATCCGCCGAACGAAGCGACAAAAACGGAGCTTCTGCTCATCGAAACCCCGAGCG
GCGCGCCGGGTGAAGAGGCTGAAGAGCTTGAGGCGGTCCAGCTTGAAGCAAGAGCGATGGCCGGGCAGATCCGCAGGCTG
ATTACAGAAAAATTTCAAGTATATGATGCCAAAGCGAAAGCGTCGCGAAACATCCAGTACCGTGATATCGTGGTGCTTTT
GCGGTCGATGCCTTGGGCGCCTCAAATTATGGACGAGTTTAAACAGCAGGGAATACCCGTATACGCGAACCTCTCAACTG
GTTATTTTGAGGCGACAGAAGTATCGGTCACACTGTCTTTATTAAAAATCATCGATAATGCCTATCAGGACATCCCGCTT
GCGTCCGTTCTCAGATCGCCTGTTGTGGGTCTTGATGAGAATGAGCTTTCCTTGATCAGAATCAAAGATAAAAAAGCGCC
GTTTTACGAAGCCATGAAAGCGTACCTTGCTGCAGCAGACGGCGATGAGAGGCTCAGCGAAAAGCTTCGCCGCTTTGACG
GTCTGCTCAAGAAGTGGAGAGCTTATGCGAAAAACCACTCTGTAGCAGAGCTGATCTGGGAAATCTACCGGGATACGAAG
TATTTGGATTATGTCGGCGGAATGCCGGGCGGAAAACAGCGGCAGGCCAATTTGCGCGCGCTTTACGACAGGGCGAGATC
CTATGAAGCGACATCATTCCGCGGCTTGTTCCGCTTTTTAAGATTTATTGAGCGGATGCAGGAGCGGGGCGATGACCTCG
GAACGGCAAGGGCTCTAAGCGAACAGGAAGACGTTGTTCGGCTGATGACGATCCACTCAAGCAAAGGGCTTGAATTTCCT
GTCGTATTTACCGCCGGTCTCGGACGGAATTTTAATATGATGGACTTGAACAAATCTTATCTGCTGGATAAAGAGCTCGG
TTTTGGAACGAAGTTTATCCATCCGAAGTGGCGGATCAGCTATCCGACGCTGCCGCTTATCGCGATGAAGAAAAAACTCC
GCCGCGAGCTTTTATCAGAGGAGCTGCGCGTCTTGTATGTCGCCCTTACCCGGGCCAAGGAAAAGCTGTACCTGGTCGGG
ACGGCGAAGGATAAAGAAAAGCTGCTTGCCGACTGGCGGACACAAGCGGCCGGGTCCGAGTGGCTGCTCCCGGATTATGA
ACGGTTTCAGGCAAAATCATATCTGGATTTCATCGGACCTGCTTTGATGAGGCACAGAGATATGGATGAATCCGGGGCGC
CGCCTGTGATTGATGAGATCCGCGAACATCCGGCGCGCTTTCAGGTCAGCTGGCTGTCCGCCGCGGATCTACAAGCTGAG
GCTGTCGATCAGGCCGGTGAAGAAAGACACGACCGGCTCGTCCAAATTCAAATGGGCCATGCGATTGAAGGCGCCTTTGA
ATATGAGCAGCAAGTCAGAGAACGACTGGCATGGTCATATCCATACAAGGACGCCGCCAAAGTCAGAACAAAACAATCTG
TCTCAGAAATGAAACGGCAGAAGGAATACGAGGATGAATACGGCGACCGCTCGCTTATTCGTCCATCACAGGAGGCTCTC
CTGTTTAAACGGCCGTCCTTTATGATGGCAAAAGGCCTGACCGCAGCCGAACGGGGGACGGCGATGCACACCGTCATGCA
GCATATTCCGCTGACCCGTACGCCTGAAAAGAATGAGCTCAGCCGCCTTCTAGACAGGCTTGTTGAGAAAGAGCTGTTAA
CAGAAGACCAGCGGGCAGCGATTGAAGAAGACGACATTCTTGCATTCTTTGATACGGAAATCGGACAAAAGCTTTTCGGC
GCCCGCCGCGTCGAACGGGAGGTTCCCTTTAACATGACGCTGTCCGCTAAAGAGGTTTATCCGGATCTTGAGTCAGCCGA
TGAACCTGTTTTGATACAGGGAATTATCGACTGCCTATTCGAGACGGAGGACGGTTTTTACCTTCTAGATTACAAAACCG
ACCGGATACACGGAAAGTACCGGAACGGCTTCGAAGGGGCCGAGCCGATTTTAAGAAAACGTTACGAAACACAAATTCAG
CTTTACGCACGAGCCGTTGAAACGATGATTAAAATGCCGCTCAAAGGGCGCGCGCTGTACTTTTTTGACGGAGGACACGT
GCTCTTATTTTAA

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(1051-1222)

UvrD/REP helicase N-terminal domain (UvrD-helicase)

(11-460)

UvrD-like helicase C-terminal domain (UvrD_C)

(471-880)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q65LJ9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addA Bacillus subtilis subsp. subtilis str. 168

71.707

100

0.717

Multiple sequence alignment