Detailed information NTG0331883

Bioinformatically predicted

Overview

• Name: comFA
• Type: Machinery gene
• Locus tag: EJ379_RS26525
• Genome accession: NZ_CP034551
• Coordinates: 5096038..5097387 (-)
• Length: 449 a.a.
• NCBI ID: WP_000225360.1
• Uniprot ID: -
• Organism: Bacillus cereus ATCC 14579
• Function: ssDNA transport into the cell (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
IScluster/Tn	5097626..5099079	5096038..5097387	flank	239

Gene organization within MGE regions

Location: 5096038..5099079

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EJ379_RS26525 (EJ379_26515)	comFA	5096038..5097387 (-)	1350	WP_000225360.1	ATP-dependent helicase ComFA	Machinery gene
EJ379_RS26530 (EJ379_26520)	-	5097626..5098705 (-)	1080	WP_000084148.1	RNA-guided endonuclease TnpB family protein	-

Sequence

Protein

• Length: 449 a.a.
• Molecular weight: 51100.00 Da
• Isoelectric Point: 10.0343

>NTDB_id=331883 EJ379_RS26525 WP_000225360.1 5096038..5097387(-) (comFA) [Bacillus cereus ATCC 14579]
MVAGKQLLLEELSSDLQRKLNDLKKKGEIVCVQGVKKKNSKYMCQRCGNVDRRLFASFLCKRCSKVCTYCRKCITMGRVS
ECAVLVRGIAERKREKNSNLLQWNGTLSTGQNLAAQGVIEAIKQKESFFIWAVCGAGKTEMLFYGINEALQKGERVCIAT
PRTDVVLELAPRLQKVFPYIKVAALYGGSVDKEKDAVLVVATTHQLLRYYRAFHVIVVDEIDAFPYCADQMLQYAVKQAM
KEKAARIYLTATPDETWKRKFRKGEQKGVIVSGRYHRHPLPVPLFCWCGNWKKSLNRERIPRVLLQWLKMYLNKKYPVFL
FVPHVRYIEEISSLLKSLHNKVEGVHAEDPMRKEKVAAFRKGEIPLLVTTTILERGVTVINLQVAVLGAEEEIFSESALV
QIAGRAGRSFEAPYGEVIYFHYGKTEAMVRAKKHIQGMNKNAKEQGLID

Nucleotide

• Length: 1350 bp

>NTDB_id=331883 EJ379_RS26525 WP_000225360.1 5096038..5097387(-) (comFA) [Bacillus cereus ATCC 14579]
ATGGTAGCGGGAAAACAGTTGCTATTAGAAGAACTTTCTTCAGATTTACAGAGGAAATTAAACGATTTGAAAAAAAAGGG
AGAGATCGTATGTGTACAAGGTGTAAAAAAGAAGAATTCTAAATATATGTGCCAACGCTGTGGAAATGTAGATCGGCGGC
TATTTGCGTCGTTTTTATGTAAAAGATGCAGTAAAGTGTGCACATATTGCCGGAAGTGTATAACGATGGGGAGAGTAAGT
GAATGTGCTGTACTTGTTCGCGGGATTGCTGAAAGAAAGAGAGAAAAGAATTCAAATTTGTTACAGTGGAACGGGACGTT
GTCTACTGGCCAGAATTTGGCGGCGCAAGGAGTTATAGAGGCTATTAAGCAAAAAGAATCATTTTTTATTTGGGCTGTAT
GCGGGGCTGGGAAAACAGAGATGTTGTTTTACGGTATTAACGAAGCGCTTCAAAAAGGAGAAAGAGTTTGTATCGCAACG
CCAAGAACGGATGTTGTTTTGGAATTAGCACCGAGATTACAAAAAGTATTTCCATATATAAAGGTAGCGGCTTTATATGG
AGGGAGTGTAGATAAAGAAAAAGATGCAGTACTAGTCGTTGCGACCACGCATCAATTATTACGTTATTATAGGGCGTTTC
ATGTCATAGTTGTAGATGAGATAGATGCTTTTCCATATTGTGCAGATCAAATGTTACAGTACGCGGTAAAACAAGCGATG
AAAGAAAAAGCGGCGCGTATTTATTTAACTGCGACTCCAGATGAAACGTGGAAGCGAAAATTTAGAAAAGGTGAACAAAA
AGGTGTTATTGTTTCTGGACGATATCACCGTCATCCGTTGCCAGTTCCCCTATTTTGCTGGTGCGGAAATTGGAAGAAAA
GCCTCAACCGTGAAAGAATTCCTCGAGTTTTACTACAATGGTTAAAGATGTACTTAAACAAAAAGTATCCTGTTTTTTTA
TTCGTTCCCCATGTACGATATATAGAAGAAATAAGCTCGTTATTGAAATCATTGCATAATAAAGTTGAAGGGGTACATGC
AGAAGATCCGATGAGAAAAGAGAAAGTCGCAGCGTTCAGAAAGGGAGAAATCCCATTATTAGTTACAACGACAATTTTAG
AGCGAGGCGTAACGGTGATAAATTTGCAAGTTGCAGTTTTAGGGGCGGAAGAAGAAATATTTTCAGAAAGTGCGCTCGTA
CAAATTGCGGGCCGAGCAGGGCGGAGCTTTGAAGCACCGTATGGAGAGGTCATTTATTTTCACTATGGCAAGACAGAGGC
GATGGTGCGCGCGAAAAAACATATTCAAGGTATGAATAAAAATGCGAAAGAACAAGGATTGATCGATTAA

Domains

Predicted by InterproScan.

• Type III restriction enzyme, res subunit (ResIII) (107-254)

• Helicase conserved C-terminal domain (Helicase_C) (313-408)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comFA	Bacillus subtilis subsp. subtilis str. 168	52.632	93.096	0.49