Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   EJJ34_RS14035 Genome accession   NZ_CP034484
Coordinates   2647614..2648024 (-) Length   136 a.a.
NCBI ID   WP_009967785.1    Uniprot ID   A0A6I4D881
Organism   Bacillus subtilis subsp. subtilis NCIB 3610 = ATCC 6051 = DSM 10 strain NCIB 3610     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2642614..2653024
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EJJ34_RS14005 (EJJ34_14010) - 2642701..2642841 (+) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -
  EJJ34_RS14010 (EJJ34_14015) yqeF 2643147..2643878 (-) 732 WP_003229964.1 SGNH/GDSL hydrolase family protein -
  EJJ34_RS14015 (EJJ34_14020) cwlH 2644130..2644882 (-) 753 WP_003229963.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  EJJ34_RS14020 (EJJ34_14025) yqeD 2645069..2645695 (+) 627 WP_003229962.1 TVP38/TMEM64 family protein -
  EJJ34_RS14025 (EJJ34_14030) gnd 2645714..2646607 (-) 894 WP_003229961.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  EJJ34_RS14030 (EJJ34_14035) yqeB 2646859..2647581 (+) 723 WP_010886572.1 hypothetical protein -
  EJJ34_RS14035 (EJJ34_14040) nucA/comI 2647614..2648024 (-) 411 WP_009967785.1 sporulation-specific Dnase NucB Machinery gene
  EJJ34_RS14040 (EJJ34_14045) - 2648220..2648567 (+) 348 Protein_2701 sigma-70 family RNA polymerase sigma factor -
  EJJ34_RS14045 (EJJ34_14050) spoIVCA 2648598..2650058 (-) 1461 WP_223257626.1 site-specific DNA recombinase SpoIVCA -
  EJJ34_RS22995 (EJJ34_14055) - 2650016..2650194 (-) 179 Protein_2703 hypothetical protein -
  EJJ34_RS14055 (EJJ34_14060) arsC 2650549..2650968 (-) 420 WP_004398596.1 thioredoxin-dependent arsenate reductase -
  EJJ34_RS14060 (EJJ34_14065) acr3 2650980..2652020 (-) 1041 WP_004398718.1 arsenite efflux transporter Acr3 -
  EJJ34_RS14065 (EJJ34_14070) arsK 2652043..2652483 (-) 441 WP_003229954.1 ArsI/CadI family heavy metal resistance metalloenzyme -
  EJJ34_RS14070 (EJJ34_14075) arsR 2652544..2652861 (-) 318 WP_004399122.1 arsenical resistance operon transcriptional regulator ArsR -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14967.97 Da        Isoelectric Point: 5.1853

>NTDB_id=331674 EJJ34_RS14035 WP_009967785.1 2647614..2648024(-) (nucA/comI) [Bacillus subtilis subsp. subtilis NCIB 3610 = ATCC 6051 = DSM 10 strain NCIB 3610]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=331674 EJJ34_RS14035 WP_009967785.1 2647614..2648024(-) (nucA/comI) [Bacillus subtilis subsp. subtilis NCIB 3610 = ATCC 6051 = DSM 10 strain NCIB 3610]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGGGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGTGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGTACCAGAGTGCTGTTTA
TTGTGCAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6I4D881

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529

Multiple sequence alignment