Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   FGL73_RS00500 Genome accession   NZ_CP042408
Coordinates   86467..88695 (+) Length   742 a.a.
NCBI ID   WP_146977844.1    Uniprot ID   -
Organism   Lactococcus lactis strain CBA3619     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 81467..93695
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FGL73_RS00465 (FGL73_00465) - 81957..82625 (+) 669 WP_058205279.1 hypothetical protein -
  FGL73_RS00470 (FGL73_00470) - 82795..83691 (+) 897 WP_003129543.1 SPFH domain-containing protein -
  FGL73_RS00480 (FGL73_00480) - 84066..84326 (+) 261 WP_003129544.1 SemiSWEET family transporter -
  FGL73_RS00490 (FGL73_00490) rnz 84683..85606 (+) 924 WP_146977843.1 ribonuclease Z -
  FGL73_RS00495 (FGL73_00495) - 85599..86300 (+) 702 WP_015425952.1 SDR family oxidoreductase -
  FGL73_RS00500 (FGL73_00500) recJ 86467..88695 (+) 2229 WP_146977844.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  FGL73_RS00505 (FGL73_00505) - 88818..89330 (+) 513 WP_012897343.1 adenine phosphoribosyltransferase -
  FGL73_RS00510 (FGL73_00510) rpoE 89547..90110 (+) 564 WP_003129551.1 DNA-directed RNA polymerase subunit delta -
  FGL73_RS00515 (FGL73_00515) mltG 90247..91899 (+) 1653 WP_038599604.1 endolytic transglycosylase MltG -
  FGL73_RS00520 (FGL73_00520) greA 91960..92433 (+) 474 WP_025016857.1 transcription elongation factor GreA -
  FGL73_RS00525 (FGL73_00525) - 92610..93065 (+) 456 WP_010905471.1 CtsR family transcriptional regulator -

Sequence

Protein


Download         Length: 742 a.a.        Molecular weight: 83578.75 Da        Isoelectric Point: 4.7551

>NTDB_id=331553 FGL73_RS00500 WP_146977844.1 86467..88695(+) (recJ) [Lactococcus lactis strain CBA3619]
MIKAKYDWKVADTVISEDFLKIAKKHKLDELTSRVLYQRGIHAEAEIEQFLKPSLENLHDPFLLHDMEKATERILSAIEQ
GENILIYGDYDADGMTGASVMKSALDELGADAQVYLPNRFTDGYGPNLDVYQYYIKNENINLIITVDNGVAGLEAITWAQ
ENGVDVIVTDHHSIPDQLPPAYAIVHPEHPDSQYPFKYLAGVGVAFKVACALLEYAPSEMLDLVTIGTIADMVSLTDENR
ILVAHGLKVLAQTERAGLQELMKYAGVDFDKITEETVGFQIAPRLNALGRLDDPNPAIELLTGWDEDEAHEIAKMIDQKN
SERKEIVEKIHNEALSMLTDEPVQILYHKDWHKGVLGIVAGRLLEAIHKPVIMLAQEEGILRGSARSIENFDIFKALNAH
RELFIAFGGHKQAAGMTLSLENVEAVKKAMIDYIVDNHLDMSKKSPLEIADRCHLDDISLSTITNLAKLAPFGMDNPKPR
FLIEDYKVIQSRSMGKDNAHLKLKIQEEKQQIDAVYFQHGSEELEFEQAQTKLVATLSSNSWNGNTSLQLMIEDADSVGV
ELLDIRSKQIPIPKEANIFSQNQLKHGIMEDVLVIEETPGDLAGLSVLKEAVSKAAPDDFKMIYFKNKITDSYYLTGSGT
REEFARLYKAIYQFPEFDIRYKLKSLADYLKIPHLLLVKMIKIFEELEFVSIDNGLMTVNKTAGKREISESTIYQELEKI
VKMQELFALAPVKEIYQNLLEK

Nucleotide


Download         Length: 2229 bp        

>NTDB_id=331553 FGL73_RS00500 WP_146977844.1 86467..88695(+) (recJ) [Lactococcus lactis strain CBA3619]
ATGATAAAAGCAAAATATGATTGGAAAGTGGCTGATACAGTCATTTCCGAAGATTTTCTAAAGATAGCGAAAAAACATAA
ATTAGATGAATTGACAAGTCGCGTGCTTTATCAAAGAGGAATTCATGCAGAAGCTGAAATTGAGCAATTTTTAAAGCCAA
GTTTAGAAAATTTACATGACCCTTTTCTACTTCATGATATGGAAAAAGCAACAGAGCGTATTTTATCAGCAATTGAGCAA
GGTGAAAATATCTTAATTTATGGTGACTATGATGCTGACGGAATGACTGGAGCATCAGTCATGAAGTCCGCTCTGGACGA
ATTAGGAGCTGATGCTCAAGTTTATTTACCCAATCGTTTTACTGACGGTTACGGGCCAAATCTTGACGTTTATCAGTACT
ATATTAAGAATGAAAATATCAATCTTATTATTACTGTAGATAATGGAGTTGCTGGCTTGGAGGCAATTACCTGGGCACAG
GAAAATGGAGTGGATGTAATTGTTACCGATCATCACTCAATTCCTGACCAACTTCCCCCTGCTTATGCCATTGTCCACCC
CGAACATCCTGACAGTCAATATCCTTTCAAATATTTGGCAGGAGTTGGTGTCGCCTTCAAAGTTGCCTGCGCTCTTTTAG
AATATGCACCCAGTGAAATGCTTGATTTGGTTACAATTGGTACAATTGCTGACATGGTCAGTTTGACTGATGAAAACCGA
ATTTTAGTGGCTCATGGCCTAAAAGTTTTGGCCCAAACAGAGCGTGCCGGCCTACAAGAATTAATGAAGTATGCTGGGGT
TGATTTTGATAAAATCACTGAGGAAACGGTTGGTTTTCAAATTGCTCCTCGTTTAAATGCTTTGGGACGTCTCGACGACC
CAAACCCAGCCATTGAATTGCTGACAGGTTGGGATGAAGATGAAGCGCATGAAATTGCCAAAATGATTGACCAAAAAAAT
TCAGAGCGAAAAGAAATTGTGGAAAAAATTCACAATGAAGCTCTGAGTATGCTGACAGATGAACCTGTTCAGATTTTATA
TCATAAGGACTGGCATAAAGGTGTCTTAGGAATTGTTGCGGGTCGCTTGTTAGAAGCCATACATAAGCCAGTAATCATGC
TGGCCCAAGAAGAGGGGATTTTGCGTGGGTCTGCTCGGTCAATCGAAAATTTTGATATTTTCAAGGCATTAAATGCCCAT
CGTGAACTTTTTATTGCTTTTGGTGGTCATAAACAAGCCGCAGGGATGACTTTAAGTCTGGAAAACGTAGAAGCAGTTAA
AAAGGCGATGATTGATTATATCGTGGACAATCATTTAGATATGTCTAAAAAAAGTCCCTTAGAAATTGCTGACCGTTGCC
ACCTAGACGATATTTCGCTCTCAACAATCACTAATTTAGCAAAATTGGCTCCCTTTGGCATGGATAATCCTAAGCCTCGA
TTTTTAATCGAAGATTATAAGGTGATTCAAAGCCGAAGTATGGGGAAAGACAATGCTCATCTTAAATTAAAAATTCAGGA
AGAAAAGCAACAAATTGATGCCGTTTATTTCCAACATGGTTCAGAAGAATTGGAATTCGAACAGGCACAAACAAAATTAG
TTGCAACTCTTTCAAGTAATAGTTGGAATGGAAATACGAGTCTTCAATTAATGATTGAAGATGCTGATTCAGTTGGTGTC
GAACTTCTTGATATAAGAAGTAAACAAATTCCTATCCCAAAAGAGGCTAATATTTTTTCTCAAAATCAGCTAAAACATGG
TATAATGGAAGATGTGCTTGTCATCGAAGAGACACCTGGAGATTTAGCAGGTTTGTCAGTATTAAAAGAAGCTGTCAGTA
AAGCAGCGCCGGATGATTTTAAAATGATTTATTTTAAAAATAAAATCACTGATAGCTATTATTTAACAGGAAGTGGAACA
CGAGAAGAATTTGCAAGACTTTATAAAGCAATTTATCAATTTCCAGAATTTGATATTCGCTATAAATTAAAATCACTTGC
GGATTATTTGAAAATCCCTCATCTCTTGCTTGTCAAGATGATAAAAATCTTTGAGGAACTTGAATTTGTCAGCATTGACA
ATGGCTTAATGACCGTTAATAAGACGGCTGGTAAACGCGAAATTTCTGAGAGTACAATTTATCAAGAACTAGAAAAAATT
GTAAAAATGCAAGAACTTTTTGCTTTAGCACCCGTCAAAGAAATTTATCAAAATTTATTAGAAAAATAA

Domains

Predicted by InterProScan.

RecJ OB domain (RecJ_OB)

(454-554)

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(592-737)

DHH family (DHH)

(83-228)

DHHA1 domain (DHHA1)

(317-434)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Streptococcus pneumoniae strain D39V

56.481

100

0.575
  recJ Bacillus subtilis subsp. subtilis str. 168

36.118

100

0.379

Multiple sequence alignment