Detailed information NTG0331112

Bioinformatically predicted

Overview

• Name: pilA
• Type: Machinery gene
• Locus tag: E3B71_RS15705
• Genome accession: NZ_CP042336
• Coordinates: 2763780..2765273 (-)
• Length: 497 a.a.
• NCBI ID: WP_001040654.1
• Uniprot ID: P10121
• Organism: Escherichia coli strain GZ04-0086
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2758780..2770273

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
E3B71_RS15680 (E3B71_13830)	panM	2759003..2759386 (+)	384	WP_000778785.1	aspartate 1-decarboxylase autocleavage activator PanM	-
E3B71_RS15685 (E3B71_13835)	livJ	2759585..2760688 (-)	1104	WP_001021996.1	branched chain amino acid ABC transporter substrate-binding protein LivJ	-
E3B71_RS15690 (E3B71_13840)	rpoH	2760959..2761813 (-)	855	WP_000130217.1	RNA polymerase sigma factor RpoH	-
E3B71_RS15695 (E3B71_13845)	ftsX	2762058..2763116 (-)	1059	WP_001042003.1	permease-like cell division protein FtsX	-
E3B71_RS15700 (E3B71_13850)	ftsE	2763109..2763777 (-)	669	WP_000617723.1	cell division ATP-binding protein FtsE	-
E3B71_RS15705 (E3B71_13855)	pilA	2763780..2765273 (-)	1494	WP_001040654.1	signal recognition particle-docking protein FtsY	Machinery gene
E3B71_RS15710 (E3B71_13860)	rsmD	2765423..2766019 (+)	597	WP_000743193.1	16S rRNA (guanine(966)-N(2))-methyltransferase	-
E3B71_RS15715 (E3B71_13865)	yhhL	2766009..2766278 (+)	270	WP_001295207.1	DUF1145 family protein	-
E3B71_RS15720 (E3B71_13870)	yhhM	2766281..2766640 (-)	360	WP_000042900.1	DUF2500 domain-containing protein	-
E3B71_RS15725 (E3B71_13875)	yhhN	2766781..2767407 (+)	627	WP_000964718.1	lysoplasmalogenase	-
E3B71_RS15730 (E3B71_13880)	zntA	2767481..2769679 (+)	2199	WP_046464157.1	Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase ZntA	-
E3B71_RS15735 (E3B71_13885)	tusA	2769781..2770026 (-)	246	WP_000130621.1	sulfurtransferase TusA	-

Sequence

Protein

• Length: 497 a.a.
• Molecular weight: 54513.35 Da
• Isoelectric Point: 4.1560

>NTDB_id=331112 E3B71_RS15705 WP_001040654.1 2763780..2765273(-) (pilA) [Escherichia coli strain GZ04-0086]
MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEPVKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAE
SEKAQPEAEVVAQPEPVVEETPEPVAIEREELPLPEDVNAEAVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELETALA
AEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISLFRGKKIDDDLFEELEEQLLIADV
GVETTRKIITNLTEGASRKQLRDAEALYGLLKEEMGEILAKVDEPLNVEGKAPFVILMVGVNGVGKTTTIGKLARQFEQQ
GKSVMLAAGDTFRAAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKSHLMEELKKI
VRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLDGTAKGGVIFSVADQFGIPIRYIGVGERIEDL
RPFKADDFIEALFARED

Nucleotide

• Length: 1494 bp

>NTDB_id=331112 E3B71_RS15705 WP_001040654.1 2763780..2765273(-) (pilA) [Escherichia coli strain GZ04-0086]
ATGGCGAAAGAAAAAAAACGTGGCTTTTTTTCCTGGCTGGGCTTTGGTCAAAAAGAGCAGACCCCGGAAAAAGAGACAGA
AGTTCAGAATGAACAACCGGTTGTAGAAGAAATCGTTCAGGCGCAAGAGCCTGTGAAGGCCTCTGAACAAGCCGTTGAAG
AGCAGCCGCAAGCGCATACTGAAGCCGAGGCGGAAACTTTTGCTGCCGACGTTGTGGAAGTCACTGAACAGGTTGCTGAA
AGTGAAAAAGCGCAGCCTGAAGCGGAAGTCGTTGCACAGCCGGAACCGGTCGTAGAAGAAACGCCGGAGCCAGTGGCTAT
CGAACGTGAAGAGCTGCCGTTGCCGGAAGACGTCAACGCCGAAGCGGTTTCGCCAGAAGAGTGGCAGGCTGAAGCGGAAA
CCGTAGAGATTGTCGAAGCGGCGGAAGAAGAAGCGGCTAAAGAAGAAATTACCGACGAAGAGCTGGAAACGGCGCTGGCT
GCCGAAGCGGCAGAAGAGGCGGTGATGGTGGTTCCTCCGGCAGAAGAAGAGCAGCCGGTGGAAGAAATCGCTCAGGAGCA
GGAAAAACCGACCAAAGAAGGTTTTTTCGCGCGCCTGAAACGCAGCCTGTTAAAAACCAAAGAAAATCTCGGTTCCGGAT
TTATCAGCCTGTTCCGCGGTAAAAAAATCGACGATGATCTGTTTGAGGAGCTGGAAGAGCAGCTTTTGATCGCCGATGTG
GGTGTGGAAACCACACGTAAAATTATCACCAATCTGACGGAAGGCGCATCCCGCAAGCAGCTTCGTGACGCCGAGGCGCT
CTATGGCCTGCTGAAAGAAGAGATGGGCGAGATTCTGGCGAAAGTCGATGAGCCGCTGAATGTTGAAGGCAAAGCGCCGT
TTGTGATCCTGATGGTGGGCGTCAACGGTGTGGGTAAAACCACGACGATTGGTAAGCTGGCGCGTCAGTTTGAGCAGCAG
GGTAAATCGGTGATGCTGGCGGCGGGTGATACTTTCCGTGCAGCTGCGGTTGAACAGCTTCAGGTCTGGGGTCAGCGCAA
CAATATTCCGGTGATTGCCCAGCATACCGGGGCGGATTCCGCCTCTGTTATCTTCGACGCCATTCAGGCAGCTAAAGCGC
GTAATATCGACGTCCTGATTGCCGATACAGCCGGACGCCTGCAGAACAAATCGCACCTGATGGAAGAGTTGAAGAAAATC
GTCCGCGTGATGAAGAAACTCGACGTTGAAGCGCCGCATGAAGTTATGCTGACTATTGATGCCAGCACCGGGCAGAACGC
GGTAAGCCAGGCCAAACTGTTCCATGAAGCCGTTGGCTTAACCGGCATCACGCTAACGAAACTGGACGGCACGGCGAAAG
GCGGGGTAATTTTCTCGGTGGCTGACCAGTTTGGTATCCCTATCCGCTACATTGGTGTCGGCGAACGTATTGAGGATTTG
CGTCCGTTTAAGGCGGACGACTTTATAGAGGCACTTTTTGCCCGAGAGGATTAA

Domains

Predicted by InterProScan.

• SRP54-type protein, helical bundle domain (SRP54_N) (210-275)

• SRP54-type protein, GTPase domain (SRP54) (293-493)

3D structure

• PDB: 1FTS
• PDB: 2QY9
• PDB: 2XXA
• PDB: 2YHS
• PDB: 3ZN8
• PDB: 4C7O
• PDB: 5GAD
• PDB: 5NCO
• PDB: 5NIY
• PDB: 6CQP
• PDB: 6CS8
• PDB: 6CVD
• PDB: 6DLX
• PDB: 6FPK
• PDB: 6FPR
• PDB: 6FQD
• PDB: 6N5I
• PDB: 6N5J
• PDB: 6N6N
• PDB: 6N9B
• PDB: 6NC1
• PDB: 6NC4
• PDB: 7O9H

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilA	Neisseria gonorrhoeae MS11	48.698	77.264	0.376