Detailed information    

insolico Bioinformatically predicted

Overview

Name   waaF   Type   Regulator
Locus tag   EJJ14_RS06170 Genome accession   NZ_CP034393
Coordinates   1173448..1174398 (+) Length   316 a.a.
NCBI ID   WP_002867402.1    Uniprot ID   -
Organism   Campylobacter jejuni subsp. jejuni strain CLB104     
Function   repress natural transformation (predicted from homology)   
Competence regulation

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1155651..1179210 1173448..1174398 within 0


Gene organization within MGE regions


Location: 1155651..1179210
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EJJ14_RS06090 (EJJ14_06090) pglK 1155651..1157345 (-) 1695 WP_002867213.1 ABC-type lipopolysaccharide transporter PglK -
  EJJ14_RS06095 (EJJ14_06095) galE 1157339..1158325 (-) 987 WP_002867214.1 UDP-glucose 4-epimerase GalE -
  EJJ14_RS06100 (EJJ14_06100) - 1158384..1159178 (-) 795 WP_002860221.1 3'-5' exonuclease -
  EJJ14_RS06105 (EJJ14_06105) waaC 1159245..1160273 (+) 1029 WP_002859241.1 lipopolysaccharide heptosyltransferase I -
  EJJ14_RS06110 (EJJ14_06110) htrB 1160263..1161150 (+) 888 WP_002867215.1 lipid A biosynthesis lauroyl acyltransferase HtrB -
  EJJ14_RS06115 (EJJ14_06115) - 1161147..1162697 (+) 1551 WP_002867216.1 glycosyltransferase -
  EJJ14_RS06120 (EJJ14_06120) - 1162666..1163553 (-) 888 WP_052784869.1 glycosyltransferase family 2 protein -
  EJJ14_RS06125 (EJJ14_06125) - 1163546..1164754 (-) 1209 WP_002867942.1 glycosyltransferase family 2 protein -
  EJJ14_RS06130 (EJJ14_06130) - 1164902..1165789 (-) 888 Protein_1199 glycosyltransferase family 2 protein -
  EJJ14_RS06135 (EJJ14_06135) - 1165974..1167026 (-) 1053 WP_079460573.1 glycosyltransferase family 2 protein -
  EJJ14_RS06140 (EJJ14_06140) - 1167023..1168072 (-) 1050 WP_002867408.1 glycosyltransferase -
  EJJ14_RS06145 (EJJ14_06145) glf 1168155..1169264 (-) 1110 WP_002867407.1 UDP-galactopyranose mutase -
  EJJ14_RS06150 (EJJ14_06150) galE 1169266..1170240 (-) 975 WP_002867406.1 UDP-glucose 4-epimerase GalE -
  EJJ14_RS06155 (EJJ14_06155) - 1170237..1171535 (-) 1299 WP_002867405.1 UDP-glucose/GDP-mannose dehydrogenase family protein -
  EJJ14_RS06160 (EJJ14_06160) - 1171541..1172497 (-) 957 WP_002867404.1 UDP-glucuronic acid decarboxylase family protein -
  EJJ14_RS06165 (EJJ14_06165) - 1172568..1173389 (-) 822 WP_002867403.1 glycosyltransferase family 2 protein -
  EJJ14_RS06170 (EJJ14_06170) waaF 1173448..1174398 (+) 951 WP_002867402.1 lipopolysaccharide heptosyltransferase II Regulator
  EJJ14_RS06175 (EJJ14_06175) - 1174384..1175148 (-) 765 WP_002867401.1 glycosyltransferase family 25 protein -
  EJJ14_RS06180 (EJJ14_06180) gmhA 1175148..1175708 (-) 561 WP_002867400.1 D-sedoheptulose 7-phosphate isomerase -
  EJJ14_RS06185 (EJJ14_06185) rfaE1 1175705..1177090 (-) 1386 WP_002867399.1 D-glycero-beta-D-manno-heptose-7-phosphate kinase -
  EJJ14_RS06190 (EJJ14_06190) rfaD 1177083..1178036 (-) 954 WP_002867398.1 ADP-glyceromanno-heptose 6-epimerase -
  EJJ14_RS06195 (EJJ14_06195) gmhB 1178037..1178598 (-) 562 Protein_1212 D-glycero-alpha-D-manno-heptose-1,7-bisphosphate 7-phosphatase -
  EJJ14_RS06200 (EJJ14_06200) - 1178679..1178981 (+) 303 WP_002852762.1 cytochrome c -
  EJJ14_RS06205 (EJJ14_06205) ccoS 1179004..1179210 (-) 207 WP_002852798.1 cbb3-type cytochrome oxidase assembly protein CcoS -

Sequence

Protein


Download         Length: 316 a.a.        Molecular weight: 36324.91 Da        Isoelectric Point: 10.3207

>NTDB_id=331052 EJJ14_RS06170 WP_002867402.1 1173448..1174398(+) (waaF) [Campylobacter jejuni subsp. jejuni strain CLB104]
MKIFIHLPTWLGDAVMASPALYAIKEHFKNAQFILYGSLVSTALFKEFPNSKIIIENKLSRYKQALSLRKELGKIDFSFA
FRSAFSSKIILHILKTKQRYFFDKNKHKEEHQVLKYLYFIENSLDIKAHFKDLKLPFKLKFKSPLVLKNGKKILGLNPGA
SFGSAKRWDASYFAKVALNFSQSHEILIFGAGKAEQELCNEIYQILKEQNIKVKNLCNKTTIKTLCQNIAFCDLFITNDS
GPMHISAVYKVKTVAIFGPTKFTQTSPWQNQNARWVHLDLACMPCMQKTCPLKHHKCMKDLKPEVILKAIQNLTNI

Nucleotide


Download         Length: 951 bp        

>NTDB_id=331052 EJJ14_RS06170 WP_002867402.1 1173448..1174398(+) (waaF) [Campylobacter jejuni subsp. jejuni strain CLB104]
ATGAAAATTTTTATACATCTTCCCACTTGGCTAGGCGATGCAGTGATGGCTTCACCTGCTTTATACGCTATAAAAGAACA
TTTTAAAAATGCCCAATTTATCCTTTATGGATCTTTGGTTTCCACAGCACTTTTTAAAGAATTTCCTAATTCTAAAATCA
TCATAGAAAATAAACTATCCCGTTATAAACAAGCTCTATCTTTACGCAAAGAACTTGGTAAGATCGATTTTAGCTTTGCT
TTTAGATCTGCGTTTTCTTCTAAGATTATCTTACATATTCTTAAAACAAAACAAAGATATTTTTTTGACAAAAACAAGCA
CAAAGAAGAACATCAAGTTTTAAAATACCTTTATTTTATAGAAAACTCACTTGACATAAAAGCTCATTTTAAAGACTTAA
AGCTTCCCTTTAAGCTAAAATTTAAAAGCCCTCTTGTCTTAAAAAATGGCAAAAAAATTCTAGGACTCAACCCTGGCGCA
AGCTTTGGAAGTGCAAAAAGATGGGATGCGAGTTATTTTGCTAAAGTGGCTTTAAATTTCAGCCAAAGTCATGAGATTTT
AATCTTTGGCGCAGGAAAAGCCGAACAAGAACTTTGTAATGAAATTTATCAAATTTTAAAAGAACAAAACATAAAAGTAA
AAAATCTTTGCAATAAAACCACTATCAAAACCCTTTGTCAAAATATCGCTTTTTGCGATCTTTTCATCACAAATGACAGT
GGACCTATGCACATAAGTGCGGTTTATAAGGTAAAAACCGTAGCTATTTTTGGCCCTACGAAATTTACTCAAACTTCACC
TTGGCAAAATCAAAATGCAAGATGGGTGCATTTAGATCTAGCTTGTATGCCTTGTATGCAAAAAACCTGCCCTTTAAAAC
ACCACAAATGCATGAAAGATTTAAAACCTGAAGTGATTTTAAAGGCAATTCAAAATTTAACTAATATCTAA

Domains

Predicted by InterproScan.

Glycosyltransferase family 9 (heptosyltransferase) (Glyco_transf_9)

(96-294)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  waaF Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819

92.652

99.051

0.918

Multiple sequence alignment