Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   EJA05_RS26520 Genome accession   NZ_CP034338
Coordinates   5710634..5711875 (-) Length   413 a.a.
NCBI ID   WP_407692110.1    Uniprot ID   -
Organism   Pseudomonas oryziphila strain 1257     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5705634..5716875
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EJA05_RS26505 (EJA05_26505) - 5707295..5708899 (-) 1605 WP_125465908.1 AAA family ATPase -
  EJA05_RS26510 (EJA05_26510) aroB 5708915..5710012 (-) 1098 WP_125465909.1 3-dehydroquinate synthase -
  EJA05_RS26515 (EJA05_26515) aroK 5710111..5710629 (-) 519 WP_110996384.1 shikimate kinase AroK -
  EJA05_RS26520 (EJA05_26520) pilQ 5710634..5711875 (-) 1242 WP_407692110.1 type IV pilus secretin PilQ Machinery gene
  EJA05_RS26525 (EJA05_26525) - 5711832..5712842 (-) 1011 WP_125860877.1 pilus assembly protein PilP -
  EJA05_RS26530 (EJA05_26530) - 5712839..5713375 (-) 537 WP_125860879.1 PilN domain-containing protein -
  EJA05_RS26535 (EJA05_26535) pilM 5713375..5714265 (-) 891 WP_125860881.1 type IV pilus biogenesis protein PilM -

Sequence

Protein


Download         Length: 413 a.a.        Molecular weight: 44949.59 Da        Isoelectric Point: 4.7414

>NTDB_id=330799 EJA05_RS26520 WP_407692110.1 5710634..5711875(-) (pilQ) [Pseudomonas oryziphila strain 1257]
MLLLGLKPTLAPAFENEPISLNFQDVEVRAVLQVMADYVGINLVASDTVQGRISLRLDDVPWQQALDLVLRSKGLGRRQE
GNVLLVAPVAELAEQSRDARLEQALEAQLEPLQRELIPVHHARAAELAELLLASLADDGILAGRGSLSVDARTNTLVVAQ
PPERIAELRKLVAQLDVPVRQVMIEARIVEAGVDYEKALGVRWGGPLYGQNSRLGSELFVDLGVERGTSGLGVGLLRSDI
LLDLELSAMEKSGNGEIISQPKVVTADKETARIIKGTEVPYQETTKSGATSISFREASLSLEVTPQITPDGQVVMEVRVT
KDEPDFVNALNNVPPIRKNEVNAKVRVADGQTIVIGGVYSTSQNNVVDKVPFLGDLPYVGRLFRRDALQEKKSELLVFLT
PRIMSDQAIAVSR

Nucleotide


Download         Length: 1242 bp        

>NTDB_id=330799 EJA05_RS26520 WP_407692110.1 5710634..5711875(-) (pilQ) [Pseudomonas oryziphila strain 1257]
ATGCTCCTGCTGGGCTTGAAGCCGACGCTGGCGCCGGCGTTTGAGAACGAGCCCATATCGCTGAACTTCCAGGACGTGGA
AGTGCGCGCAGTGCTGCAGGTCATGGCCGACTACGTGGGGATCAACCTGGTGGCCAGCGATACGGTCCAGGGCCGCATCA
GCCTGCGCCTTGACGATGTGCCGTGGCAGCAGGCGCTGGATCTGGTGCTGCGCAGCAAAGGGCTGGGGCGGCGCCAGGAG
GGTAATGTGCTGCTGGTCGCGCCGGTTGCCGAGCTTGCCGAGCAGTCGCGCGATGCCCGCCTGGAGCAGGCGCTGGAGGC
GCAACTGGAACCCTTGCAGCGCGAGCTGATTCCGGTCCATCACGCCCGGGCTGCGGAACTGGCCGAGTTGCTGCTGGCCA
GCCTTGCCGATGACGGCATCCTGGCCGGGCGCGGCAGCCTCAGCGTGGATGCACGGACCAATACCCTGGTGGTGGCCCAG
CCGCCAGAGCGGATCGCCGAGCTGCGCAAGCTGGTGGCCCAGCTCGATGTGCCCGTGCGCCAGGTGATGATCGAGGCGCG
CATCGTCGAGGCCGGCGTCGATTACGAAAAGGCACTGGGTGTGCGTTGGGGAGGCCCTTTGTACGGGCAGAACAGCCGGT
TGGGCAGCGAGCTGTTCGTCGACCTGGGGGTGGAGCGGGGTACGTCGGGGCTGGGTGTGGGCCTGCTGCGCAGTGACATC
CTGCTGGACCTGGAGCTCAGTGCCATGGAAAAGAGCGGTAACGGCGAGATCATCTCGCAGCCCAAGGTGGTCACCGCCGA
CAAAGAGACCGCGCGCATCATCAAGGGCACCGAGGTGCCCTACCAGGAGACGACCAAGAGTGGTGCCACCTCGATCTCGT
TCCGCGAGGCCTCGCTGTCGCTGGAAGTAACGCCGCAGATCACCCCGGATGGCCAGGTGGTGATGGAGGTGCGGGTGACC
AAGGATGAGCCGGATTTCGTCAATGCCCTGAACAATGTGCCGCCGATCCGCAAGAATGAGGTCAATGCCAAGGTGCGGGT
GGCCGATGGCCAGACCATTGTGATTGGCGGTGTCTACTCGACGTCGCAGAACAATGTGGTCGACAAGGTGCCATTTTTGG
GCGATCTGCCGTATGTTGGGCGGCTGTTTCGACGCGATGCATTACAAGAGAAAAAATCCGAGCTGCTGGTCTTCCTGACT
CCGCGTATCATGAGTGACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(42-88)

Bacterial type II and III secretion system protein (Secretin)

(248-404)

Bacterial type II/III secretion system short domain (Secretin_N)

(116-181)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

57.895

100

0.613
  comQ Acinetobacter baylyi ADP1

44.989

100

0.489
  pilQ Acinetobacter baumannii strain A118

41.649

100

0.465
  pilQ Acinetobacter baumannii D1279779

41.649

100

0.465
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

42.453

100

0.436
  pilQ Vibrio cholerae strain A1552

42.453

100

0.436
  pilQ Vibrio campbellii strain DS40M4

42.453

100

0.436
  pilQ Legionella pneumophila strain Lp02

43.062

100

0.436
  pilQ Legionella pneumophila strain ERS1305867

43.062

100

0.436
  pilQ Neisseria gonorrhoeae strain FA1090

37.53

100

0.383
  pilQ Neisseria meningitidis MC58

36.905

100

0.375
  pilQ Neisseria meningitidis M1080

36.667

100

0.373

Multiple sequence alignment