Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   EI562_RS16430 Genome accession   NZ_CP034336
Coordinates   3279639..3281072 (-) Length   477 a.a.
NCBI ID   WP_023309360.1    Uniprot ID   A0A1Z3N130
Organism   Enterobacter asburiae strain CAV1043     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3274639..3286072
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EI562_RS16400 (EI562_16400) elbB 3276171..3276824 (+) 654 WP_032667950.1 isoprenoid biosynthesis glyoxalase ElbB -
  EI562_RS16405 (EI562_16405) mtgA 3276821..3277546 (+) 726 WP_023309361.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  EI562_RS16410 (EI562_16410) npr 3277586..3277858 (-) 273 WP_003861872.1 PTS phosphocarrier protein NPr -
  EI562_RS16415 (EI562_16415) rapZ 3277855..3278709 (-) 855 WP_014885397.1 RNase adapter RapZ -
  EI562_RS16420 (EI562_16420) ptsN 3278755..3279246 (-) 492 WP_008501443.1 PTS IIA-like nitrogen regulatory protein PtsN -
  EI562_RS16425 (EI562_16425) hpf 3279329..3279616 (-) 288 WP_008501444.1 ribosome hibernation promoting factor -
  EI562_RS16430 (EI562_16430) rpoN/rpoN1 3279639..3281072 (-) 1434 WP_023309360.1 RNA polymerase factor sigma-54 Machinery gene
  EI562_RS16435 (EI562_16435) lptB 3281120..3281845 (-) 726 WP_023309359.1 LPS export ABC transporter ATP-binding protein -
  EI562_RS16440 (EI562_16440) lptA 3281852..3282406 (-) 555 WP_023309358.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  EI562_RS16445 (EI562_16445) lptC 3282375..3282950 (-) 576 WP_023309357.1 LPS export ABC transporter periplasmic protein LptC -
  EI562_RS16450 (EI562_16450) kdsC 3282947..3283513 (-) 567 WP_023309356.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  EI562_RS16455 (EI562_16455) kdsD 3283528..3284514 (-) 987 WP_023309355.1 arabinose-5-phosphate isomerase KdsD -
  EI562_RS16460 (EI562_16460) - 3284528..3285505 (-) 978 WP_023309354.1 calcium/sodium antiporter -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 54169.89 Da        Isoelectric Point: 4.3149

>NTDB_id=330747 EI562_RS16430 WP_023309360.1 3279639..3281072(-) (rpoN/rpoN1) [Enterobacter asburiae strain CAV1043]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALDSNPLLEQTDLHDEVDTQQSQDTEALDTADALEQKEMPD
ELPLDASWDEIYTAGTPSGTRADYQDDELPVYQGETTQSLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVTLDD
ILESMGDDEIELEEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFSKETPWIDEARLIISDHLDLLANHDFRSLMRVTR
LKEEVLKEAVNLIQSLDPRPGQSIQTSEPEYVIPDVLVRKHNDRWVVELNSDSIPRLQINQQYASMCTSARNDSDNQYIR
SNLQEARWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTTMLSDQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=330747 EI562_RS16430 WP_023309360.1 3279639..3281072(-) (rpoN/rpoN1) [Enterobacter asburiae strain CAV1043]
ATGAAGCAAGGTTTGCAATTAAGGCTCAGCCAACAACTGGCGATGACGCCGCAGCTACAGCAGGCAATTCGCCTGTTGCA
GCTGTCCACGTTAGAACTTCAGCAGGAGCTCCAGCAGGCGCTGGACAGCAACCCCCTGCTGGAACAAACCGATCTTCATG
ACGAGGTAGATACTCAGCAGTCACAGGATACAGAAGCGCTCGACACCGCGGATGCACTCGAACAAAAAGAGATGCCCGAC
GAGCTTCCGCTGGATGCCAGCTGGGATGAAATCTACACCGCCGGAACCCCTTCCGGTACGCGTGCAGACTACCAGGATGA
TGAGCTACCGGTCTACCAGGGTGAGACCACTCAGTCGCTGCAGGATTACCTGATGTGGCAGGTGGAACTCACCCCCTTCT
CCGATACCGACCGCGCGATTGCCACGTCCATTGTCGATGCCGTTGACGACACCGGCTATTTGACCGTCACGCTGGACGAT
ATTCTGGAAAGCATGGGCGACGACGAGATTGAGCTTGAGGAAGTCGAAGCCGTTCTGAAGCGCATCCAGCGTTTCGATCC
GGTAGGCGTGGCCGCAAAAGATCTGCGTGACTGTCTGCTGATCCAGCTTTCTCAGTTCAGCAAAGAGACGCCATGGATCG
ACGAGGCGCGCTTAATCATCAGCGATCATCTGGATCTGTTGGCCAACCACGATTTCCGCAGCCTGATGCGCGTCACGCGC
CTGAAGGAAGAGGTGCTGAAAGAAGCGGTGAATCTGATCCAGTCGCTCGATCCGCGCCCGGGACAGTCGATCCAGACCAG
CGAGCCCGAGTACGTCATCCCGGACGTGCTGGTCAGAAAACACAATGACCGCTGGGTCGTTGAACTGAACTCAGACAGCA
TCCCTCGCCTGCAAATCAACCAGCAGTATGCCTCCATGTGCACCAGCGCGCGTAACGACTCCGACAATCAATATATTCGC
AGCAATCTTCAGGAAGCGCGATGGCTGATCAAAAGTCTGGAGAGCCGAAATGATACGCTGCTACGCGTCAGCCGCTGTAT
TGTCGAACAGCAGCAGGCGTTCTTTGAGCAGGGCGAAGAGTATATGAAACCGATGGTACTGGCGGATATCGCCCAGGCCG
TCGAGATGCATGAATCAACCATTTCCCGTGTCACCACGCAGAAGTATCTGCACAGTCCACGCGGTATTTTTGAGCTTAAG
TATTTCTTCTCCAGCCATGTGAATACCGAGGGCGGCGGCGAAGCGTCGTCAACGGCCATTCGTGCCCTGGTGAAGAAGTT
GATCGCCGCGGAGAACCCCGCGAAGCCACTAAGCGACAGTAAGTTAACCACCATGCTGTCCGATCAGGGTATCATGGTGG
CACGTCGTACTGTTGCGAAGTATCGAGAGTCTTTATCCATTCCGCCGTCCAACCAGCGTAAACAGCTGGTCTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1Z3N130

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.347

100

0.48

Multiple sequence alignment