Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   FQU83_RS22210 Genome accession   NZ_CP042246
Coordinates   4106066..4106506 (+) Length   146 a.a.
NCBI ID   WP_000360914.1    Uniprot ID   A0A0H2YVZ3
Organism   Escherichia coli strain PU-1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4101066..4111506
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FQU83_RS22190 (FQU83_22195) aroP 4102066..4103436 (+) 1371 WP_000399017.1 aromatic amino acid transporter AroP -
  FQU83_RS22195 (FQU83_22200) ampE 4103479..4104333 (-) 855 WP_000171983.1 beta-lactamase regulator AmpE -
  FQU83_RS22200 (FQU83_22205) ampD 4104330..4104881 (-) 552 WP_000923703.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  FQU83_RS22205 (FQU83_22210) nadC 4104969..4105862 (+) 894 WP_001135168.1 carboxylating nicotinate-nucleotide diphosphorylase -
  FQU83_RS22210 (FQU83_22215) pilA 4106066..4106506 (+) 441 WP_000360914.1 prepilin peptidase-dependent pilin Machinery gene
  FQU83_RS22215 (FQU83_22220) pilB 4106516..4107901 (+) 1386 WP_001025178.1 type II secretion system protein GspE Machinery gene
  FQU83_RS22220 (FQU83_22225) hofC 4107891..4109093 (+) 1203 WP_000157254.1 protein transport protein HofC -
  FQU83_RS22225 (FQU83_22230) guaC 4109128..4110171 (-) 1044 WP_001217338.1 GMP reductase -
  FQU83_RS27380 - 4110327..4110371 (-) 45 WP_120795372.1 protein YacM -
  FQU83_RS22230 (FQU83_22235) coaE 4110396..4111016 (+) 621 WP_001269535.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15620.77 Da        Isoelectric Point: 4.5237

>NTDB_id=330525 FQU83_RS22210 WP_000360914.1 4106066..4106506(+) (pilA) [Escherichia coli strain PU-1]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLNTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=330525 FQU83_RS22210 WP_000360914.1 4106066..4106506(+) (pilA) [Escherichia coli strain PU-1]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAAATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGACTAAGCGTCGTCAT
GACGCCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGTAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2YVZ3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363