Detailed information NTG0330275

Bioinformatically predicted

Overview

• Name: ccrA
• Type: Machinery gene
• Locus tag: EFD74_RS00310
• Genome accession: NZ_CP034259
• Coordinates: 58498..59847 (-)
• Length: 449 a.a.
• NCBI ID: WP_000815646.1
• Uniprot ID: Q9XB96
• Organism: Staphylococcus aureus strain P1D12C1
• Function: promote SCCmec transfer (predicted from homology) Homologous recombination

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
SCCmec	38568..59847	58498..59847	within	0

Gene organization within MGE regions

Location: 38568..59847

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EFD74_RS00200 (EFD74_00215)	mecA	38568..40577 (-)	2010	WP_001801873.1	PBP2a family beta-lactam-resistant peptidoglycan transpeptidase MecA	-
EFD74_RS00205 (EFD74_00220)	mecR1	40674..42431 (+)	1758	WP_156667966.1	beta-lactam sensor/signal transducer MecR1	-
EFD74_RS00210 (EFD74_00225)	mecI	42431..42802 (+)	372	WP_000369214.1	mecA-type methicillin resistance repressor MecI	-
EFD74_RS00215 (EFD74_00230)	psm-mec	42887..42955 (-)	69	WP_014532405.1	phenol-soluble modulin PSM-mec	-
EFD74_RS00220 (EFD74_00235)	-	43289..44419 (+)	1131	WP_010922808.1	ROK family transcriptional regulator	-
EFD74_RS00225 (EFD74_00240)	cstB	44533..45859 (-)	1327	Protein_34	persulfide dioxygenase-sulfurtransferase CstB	-
EFD74_RS00230 (EFD74_00245)	-	45892..46956 (-)	1065	WP_000438836.1	DsrE/DsrF/DrsH-like family protein	-
EFD74_RS00235 (EFD74_00250)	cstR	47092..47352 (+)	261	WP_000220507.1	persulfide-sensing transcriptional repressor CstR	-
EFD74_RS00240 (EFD74_00255)	-	47352..47990 (+)	639	Protein_37	sulfite exporter TauE/SafE family protein	-
EFD74_RS00250 (EFD74_00265)	-	48335..48997 (-)	663	WP_001092058.1	class I SAM-dependent methyltransferase	-
EFD74_RS14215 (EFD74_00275)	-	49389..49448 (+)	60	WP_010959190.1	erythromycin resistance leader peptide	-
EFD74_RS00260 (EFD74_00280)	erm(A)	49531..50262 (+)	732	WP_001072201.1	23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(A)	-
EFD74_RS00265 (EFD74_00285)	-	50388..51170 (-)	783	WP_000067268.1	aminoglycoside nucleotidyltransferase ANT(9)-Ia	-
EFD74_RS00270 (EFD74_00290)	-	51321..51698 (-)	378	WP_000361059.1	DUF6262 family protein	-
EFD74_RS00275 (EFD74_00295)	-	51705..53597 (-)	1893	WP_001557544.1	site-specific integrase	-
EFD74_RS00280 (EFD74_00300)	-	53594..54679 (-)	1086	WP_000868132.1	tyrosine-type recombinase/integrase	-
EFD74_RS00285 (EFD74_00305)	-	54720..55037 (-)	318	WP_000659050.1	JAB domain-containing protein	-
EFD74_RS00290 (EFD74_00310)	-	55058..55564 (-)	507	WP_001092169.1	DUF1643 domain-containing protein	-
EFD74_RS00295 (EFD74_00315)	-	55582..55893 (-)	312	WP_000700853.1	DUF960 family protein	-
EFD74_RS00300 (EFD74_00320)	-	55980..56330 (-)	351	WP_000859155.1	SAUGI family uracil-DNA glycosylase inhibitor	-
EFD74_RS00305 (EFD74_00325)	ccrB	56848..58476 (-)	1629	WP_156667967.1	cassette chromosome recombinase CcrB	Machinery gene
EFD74_RS00310 (EFD74_00330)	ccrA	58498..59847 (-)	1350	WP_000815646.1	cassette chromosome recombinase CcrA	Machinery gene

Sequence

Protein

• Length: 449 a.a.
• Molecular weight: 52607.20 Da
• Isoelectric Point: 9.9585

>NTDB_id=330275 EFD74_RS00310 WP_000815646.1 58498..59847(-) (ccrA) [Staphylococcus aureus strain P1D12C1]
MKQVIGYLRQSTMKQQSLAAQKQAIEAIAEKHHIQHINFYSDKQSGRKDNRSGYRQMTQLIQQGQCDILCCYRLNRLHRN
LKNALKLIKLCQTYHVHILSVHDGYFDMDQAFDRFKLNIFISLAELESDNIGEQVRNGLQEKAKQGRLITTHAPFGYEYH
NGAFIINQNESPTVKAVFNYYIKGHGYKKIAQLLEEDNTYINRQPYQVRNIIINPNYCGRVNNQYGQFDNMFPSIVSTSI
YEQAQRLRSQKQIKQTSSDNQLKQKIKCPCCNTTLTNMTVRKKNHTLRYYVCPKNMNASRFVCDFKGINAQTLEDKVLEV
CKDFYQNQRIYTKIKGAIDKRIKRQRNIEKHHTLTQEQLIEKLAQGIIDAETFREQTQSLRQQPQRTTSINGHQIQHTIQ
NIIQKRFTLNILYPYIETIHITKDKNLIGIYFKNEPLNIVNQTMQSSIA

Nucleotide

• Length: 1350 bp

>NTDB_id=330275 EFD74_RS00310 WP_000815646.1 58498..59847(-) (ccrA) [Staphylococcus aureus strain P1D12C1]
ATGAAACAAGTCATAGGCTATTTACGTCAAAGTACGATGAAACAACAATCTCTTGCAGCACAGAAACAGGCTATCGAAGC
AATAGCCGAAAAACATCATATTCAACATATCAACTTTTATAGCGACAAACAATCAGGACGCAAAGATAATCGTAGTGGGT
ATCGACAAATGACGCAATTAATTCAACAAGGGCAATGTGACATATTATGTTGTTATCGTCTTAATAGATTACATCGTAAT
CTGAAAAATGCATTAAAACTCATCAAATTATGTCAAACATACCATGTTCATATCTTAAGCGTACACGATGGTTACTTTGA
TATGGATCAAGCTTTCGACCGATTCAAGCTTAATATTTTTATCAGCTTGGCCGAACTTGAATCAGATAACATTGGAGAAC
AAGTCAGAAATGGGCTTCAAGAAAAAGCAAAGCAAGGTCGATTGATTACAACCCATGCGCCCTTTGGTTACGAATATCAC
AACGGAGCATTCATCATCAATCAAAATGAGTCACCAACGGTAAAGGCTGTATTCAATTATTACATTAAAGGTCATGGTTA
TAAGAAAATTGCACAGTTATTAGAAGAAGATAACACGTATATCAATCGACAACCCTATCAAGTTCGTAACATTATCATCA
ATCCTAATTATTGTGGTCGTGTCAACAATCAATATGGCCAATTCGACAATATGTTTCCTTCTATTGTTTCCACAAGTATA
TATGAGCAAGCGCAGAGACTTCGATCGCAAAAACAAATCAAACAGACATCTTCGGATAACCAACTAAAACAAAAAATCAA
ATGCCCATGTTGTAATACAACACTTACGAATATGACCGTTAGAAAAAAGAATCATACATTACGTTACTACGTCTGTCCTA
AAAACATGAATGCTTCTCGCTTTGTCTGTGATTTTAAAGGCATCAATGCACAAACACTTGAAGATAAAGTATTAGAAGTG
TGCAAAGACTTTTATCAAAATCAACGCATCTACACAAAAATTAAAGGTGCGATTGACAAACGCATCAAAAGACAAAGAAA
CATAGAAAAACATCACACATTGACTCAAGAACAACTGATAGAAAAGTTGGCACAAGGCATCATCGATGCAGAAACGTTCA
GAGAACAAACGCAATCATTACGTCAACAACCGCAACGCACTACATCTATCAATGGACATCAAATACAACACACCATTCAA
AATATTATTCAAAAACGTTTCACGTTAAACATATTGTACCCCTATATTGAAACCATTCACATTACGAAAGATAAAAATCT
TATAGGAATCTATTTCAAAAATGAACCACTCAATATCGTCAATCAAACCATGCAATCATCGATTGCATAA

Domains

Predicted by InterproScan.

• Recombinase (Recombinase) (169-250)

• Recombinase zinc beta ribbon domain (Zn_ribbon_recom) (262-322)

• Resolvase, N terminal domain (Resolvase) (4-147)

3D structure

• AlphaFold DB: Q9XB96

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
ccrA	Staphylococcus aureus N315	100	100	1
ccrA	Staphylococcus aureus COL	74.833	100	0.748