Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   EH197_RS01280 Genome accession   NZ_CP034169
Coordinates   159215..160588 (+) Length   457 a.a.
NCBI ID   WP_048722477.1    Uniprot ID   A0A2N8PZS7
Organism   Enterococcus avium strain 352     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 154215..165588
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EH197_RS01260 (EH197_01265) - 154857..155228 (-) 372 WP_016179415.1 TIGR02328 family protein -
  EH197_RS01265 (EH197_01270) - 155367..156323 (+) 957 WP_137438878.1 cation diffusion facilitator family transporter -
  EH197_RS01270 (EH197_01275) - 156771..158570 (+) 1800 WP_049220157.1 glycerophosphodiester phosphodiesterase -
  EH197_RS01275 (EH197_01280) - 158680..159186 (+) 507 WP_049220159.1 dUTP diphosphatase -
  EH197_RS01280 (EH197_01285) radA 159215..160588 (+) 1374 WP_048722477.1 DNA repair protein RadA Machinery gene
  EH197_RS01285 (EH197_01290) - 160679..161833 (+) 1155 WP_048722474.1 PIN/TRAM domain-containing protein -
  EH197_RS01290 (EH197_01295) gltX 161938..163395 (+) 1458 WP_049220166.1 glutamate--tRNA ligase -
  EH197_RS01295 (EH197_01300) epsC 163844..164377 (+) 534 WP_016179422.1 serine O-acetyltransferase EpsC -

Sequence

Protein


Download         Length: 457 a.a.        Molecular weight: 50009.41 Da        Isoelectric Point: 7.0289

>NTDB_id=329512 EH197_RS01280 WP_048722477.1 159215..160588(+) (radA) [Enterococcus avium strain 352]
MAKKSKTQFICQECGYVSPKYLGRCPNCGNWNTMVEEIEQNHPEDRRNRVSLTGERAQPTKLKDVVFKKETRIQTELAEL
NRVLGGGVVPGSLVLIGGDPGIGKSTLLLQVSHQLAATGGNVLYVSGEESAQQIKLRAERLGSSETDFYLYAETDMGEIS
RTIENLKPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKLAKTNGIAIFIVGHVTKEGNIAGPRMLEHMVDTVLYF
EGEKHHSFRILRAVKNRFGSTNEIGIFEMRESGLVEVANPSQVFLEERLEGATGSAIVVAMEGSRPILVEVQALVTPTVF
GNAKRTTTGLDFNRVSLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAVAVAIASSYKENGTEATECFIGEIGLT
GEIRRVSSIEQRVKEASKLGFKRIYLPKNNMGDWTPPKGIELIPVATLGETLRKVFR

Nucleotide


Download         Length: 1374 bp        

>NTDB_id=329512 EH197_RS01280 WP_048722477.1 159215..160588(+) (radA) [Enterococcus avium strain 352]
TTGGCGAAAAAATCCAAGACACAATTTATCTGCCAAGAATGCGGCTATGTTTCGCCGAAGTATCTAGGTCGTTGTCCTAA
CTGCGGAAACTGGAACACGATGGTAGAAGAAATTGAGCAAAATCACCCTGAAGATCGCCGCAATCGGGTGAGTCTGACAG
GGGAACGCGCACAACCGACGAAGCTAAAAGATGTTGTCTTTAAAAAGGAAACGCGCATTCAAACGGAATTAGCAGAATTG
AATCGGGTTTTAGGCGGCGGTGTAGTTCCAGGTTCATTGGTTTTAATTGGTGGAGACCCCGGCATCGGGAAGTCTACATT
ACTTTTACAGGTTTCGCACCAATTAGCAGCAACTGGCGGAAATGTATTATATGTTTCTGGGGAAGAAAGTGCTCAGCAGA
TAAAATTGCGAGCAGAGCGGCTAGGTTCGAGTGAGACAGACTTTTATCTGTATGCTGAAACAGATATGGGCGAGATTAGT
CGGACGATTGAAAATCTGAAACCAGATTATGTCATCATTGATTCGATCCAGACTATGACGCAGCCTGACATTACCAGTGT
AGCTGGGTCAGTCAGTCAGGTTCGTGAAACGACGGCAGAATTATTGAAGCTGGCGAAAACCAACGGTATCGCGATCTTCA
TCGTCGGGCATGTGACAAAAGAAGGAAACATCGCAGGGCCTCGAATGTTGGAGCATATGGTGGATACTGTGCTGTACTTT
GAAGGCGAAAAACATCACAGTTTTCGGATTTTAAGGGCCGTAAAAAATCGTTTTGGTTCTACTAATGAGATTGGAATTTT
TGAGATGCGAGAAAGCGGCTTAGTGGAAGTGGCTAATCCTTCTCAGGTCTTTTTAGAAGAACGTTTAGAAGGTGCGACCG
GTTCAGCGATTGTGGTGGCAATGGAGGGTAGCCGACCGATTTTAGTTGAGGTCCAGGCGTTAGTAACACCAACAGTCTTC
GGAAATGCGAAACGTACGACAACTGGATTAGACTTTAATCGCGTATCCTTGATCATGGCGGTCTTAGAAAAACGCGCCGG
CTTGCTTTTACAGAATCAAGACGCCTACTTAAAGGCTGCGGGCGGTGTGAAATTAAATGAGCCCGCAATTGATTTGGCGG
TAGCCGTGGCGATCGCTTCTAGCTACAAAGAAAATGGCACGGAAGCAACTGAATGTTTTATCGGTGAGATTGGTTTGACT
GGTGAGATTCGTCGGGTCAGCAGTATCGAGCAGCGCGTCAAAGAAGCCAGTAAGTTAGGTTTCAAAAGAATCTATCTCCC
TAAGAACAACATGGGAGATTGGACGCCGCCAAAGGGGATCGAATTGATTCCGGTGGCGACACTAGGTGAAACATTAAGAA
AAGTATTCCGTTAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(57-222)

Subunit ChlI of Mg-chelatase (ChlI)

(363-430)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-36)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2N8PZS7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis NCTC 12261

73.904

99.781

0.737
  radA Streptococcus mitis SK321

73.684

99.781

0.735
  radA Streptococcus pneumoniae Rx1

73.684

99.781

0.735
  radA Streptococcus pneumoniae D39

73.684

99.781

0.735
  radA Streptococcus pneumoniae R6

73.684

99.781

0.735
  radA Streptococcus pneumoniae TIGR4

73.684

99.781

0.735
  radA Bacillus subtilis subsp. subtilis str. 168

65.854

98.687

0.65

Multiple sequence alignment