Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   FN958_RS19315 Genome accession   NZ_CP041996
Coordinates   3977838..3978278 (+) Length   146 a.a.
NCBI ID   WP_000360914.1    Uniprot ID   A0A0H2YVZ3
Organism   Escherichia coli strain AR Bank #0349     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3972838..3983278
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FN958_RS19295 (FN958_19295) aroP 3973836..3975209 (+) 1374 WP_000969915.1 aromatic amino acid transporter AroP -
  FN958_RS19300 (FN958_19300) ampE 3975252..3976106 (-) 855 WP_000172009.1 beta-lactamase regulator AmpE -
  FN958_RS19305 (FN958_19305) ampD 3976103..3976654 (-) 552 WP_000923725.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  FN958_RS19310 (FN958_19310) nadC 3976742..3977635 (+) 894 WP_001135147.1 carboxylating nicotinate-nucleotide diphosphorylase -
  FN958_RS19315 (FN958_19315) pilA 3977838..3978278 (+) 441 WP_000360914.1 prepilin peptidase-dependent pilin Machinery gene
  FN958_RS19320 (FN958_19320) pilB 3978288..3979673 (+) 1386 WP_001025186.1 type II secretion system protein GspE Machinery gene
  FN958_RS19325 (FN958_19325) hofC 3979663..3980865 (+) 1203 WP_000157222.1 protein transport protein HofC -
  FN958_RS19330 (FN958_19330) guaC 3980900..3981943 (-) 1044 WP_001217337.1 GMP reductase -
  FN958_RS25930 - 3982099..3982143 (-) 45 WP_120795372.1 protein YacM -
  FN958_RS19335 (FN958_19335) coaE 3982168..3982788 (+) 621 WP_001269506.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15620.77 Da        Isoelectric Point: 4.5237

>NTDB_id=329329 FN958_RS19315 WP_000360914.1 3977838..3978278(+) (pilA) [Escherichia coli strain AR Bank #0349]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLNTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=329329 FN958_RS19315 WP_000360914.1 3977838..3978278(+) (pilA) [Escherichia coli strain AR Bank #0349]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAAATACCTGCGACGGTGGCAGCAATGGCATTCCTTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACGCCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2YVZ3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363