Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   EGM68_RS16340 Genome accession   NZ_CP034141
Coordinates   3726656..3727093 (-) Length   145 a.a.
NCBI ID   WP_013371920.1    Uniprot ID   A0A3G8R818
Organism   Paenibacillus sp. M-152     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3721656..3732093
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EGM68_RS16330 (EGM68_16330) - 3722952..3724577 (-) 1626 WP_129549585.1 response regulator -
  EGM68_RS16335 (EGM68_16335) - 3724552..3726372 (-) 1821 WP_013371918.1 sensor histidine kinase -
  EGM68_RS25755 - 3726487..3726663 (+) 177 WP_013371919.1 hypothetical protein -
  EGM68_RS16340 (EGM68_16340) nucA/comI 3726656..3727093 (-) 438 WP_013371920.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  EGM68_RS16345 (EGM68_16345) - 3727235..3727429 (+) 195 WP_013371921.1 hypothetical protein -
  EGM68_RS16350 (EGM68_16350) - 3727814..3728116 (+) 303 WP_013371923.1 hypothetical protein -
  EGM68_RS16355 (EGM68_16355) - 3728189..3730075 (-) 1887 WP_129549586.1 Ig-like domain-containing protein -
  EGM68_RS16360 (EGM68_16360) - 3730196..3730618 (-) 423 WP_013371925.1 hypothetical protein -
  EGM68_RS16365 (EGM68_16365) - 3730635..3731303 (-) 669 WP_129549587.1 uracil-DNA glycosylase -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 15964.19 Da        Isoelectric Point: 9.4243

>NTDB_id=329300 EGM68_RS16340 WP_013371920.1 3726656..3727093(-) (nucA/comI) [Paenibacillus sp. M-152]
MKKKLLSFIALVLLAVGAYWVEGDNLFTKITGENPPSSAQVTLQFPSGRYPETAQHIKEAIQAGKSPVCTIDREGAEQNR
KHSLAGVPTRKGFDRDEWPMAMCSEGGKGANVKYIAPKDNRGAGSWVSHQLDQYEDGTRVKFVIK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=329300 EGM68_RS16340 WP_013371920.1 3726656..3727093(-) (nucA/comI) [Paenibacillus sp. M-152]
TTGAAGAAAAAGTTACTAAGTTTTATAGCCTTAGTGCTGCTAGCTGTGGGCGCGTACTGGGTTGAAGGAGACAATCTTTT
TACAAAAATAACAGGAGAAAACCCGCCTTCATCAGCTCAGGTAACGCTGCAATTTCCGTCAGGTCGTTATCCTGAAACGG
CGCAGCATATTAAGGAAGCCATTCAAGCGGGAAAATCACCAGTATGCACAATTGACCGGGAAGGAGCCGAGCAAAATCGT
AAGCATTCGCTTGCGGGTGTTCCTACTCGTAAAGGGTTTGATCGTGATGAATGGCCGATGGCTATGTGCTCAGAAGGGGG
CAAGGGTGCAAATGTCAAATACATAGCTCCTAAGGATAACCGTGGGGCAGGATCATGGGTCAGTCACCAGTTAGATCAGT
ATGAGGATGGAACTCGTGTAAAATTCGTTATTAAATAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(48-142)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3G8R818

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

67.308

71.724

0.483

Multiple sequence alignment