Detailed information NTG0032908

Bioinformatically predicted

Overview

• Name: rapF
• Type: Regulator
• Locus tag: BAMF_RS21720
• Genome accession: NC_014551
• Coordinates: 275980..277101 (+)
• Length: 373 a.a.
• NCBI ID: WP_013350919.1
• Uniprot ID: A0A9P1NGF1
• Organism: Bacillus amyloliquefaciens DSM 7 = ATCC 23350
• Function: inhibit the DNA-binding function of ComA (predicted from homology) Competence regulation

Genomic Context

Location: 270980..282101

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BAMF_RS21700 (BAMF_0249)	-	271310..272305 (-)	996	WP_013350915.1	DUF5105 domain-containing protein	-
BAMF_RS21705 (BAMF_0250)	-	272423..273811 (+)	1389	WP_041481592.1	YcdB/YcdC domain-containing protein	-
BAMF_RS21710 (BAMF_0251)	-	274012..275370 (+)	1359	WP_013350917.1	YcdB/YcdC domain-containing protein	-
BAMF_RS21715 (BAMF_0252)	-	275367..275870 (-)	504	WP_013350918.1	M15 family metallopeptidase	-
BAMF_RS21720 (BAMF_0253)	rapF	275980..277101 (+)	1122	WP_013350919.1	tetratricopeptide repeat protein	Regulator
BAMF_RS21725 (BAMF_0254)	-	277215..277994 (+)	780	WP_013350920.1	glucose 1-dehydrogenase	-
BAMF_RS21730 (BAMF_0255)	-	278078..279757 (+)	1680	WP_013350921.1	glycoside hydrolase family 13 protein	-
BAMF_RS21735 (BAMF_0256)	-	279742..280236 (-)	495	WP_013350922.1	DUF4188 domain-containing protein	-
BAMF_RS21740 (BAMF_0257)	-	280237..280782 (-)	546	WP_013350923.1	PadR family transcriptional regulator	-
BAMF_RS21745 (BAMF_0258)	-	280991..281950 (+)	960	WP_013350924.1	metal ABC transporter substrate-binding protein	-

Sequence

Protein

• Length: 373 a.a.
• Molecular weight: 44347.46 Da
• Isoelectric Point: 5.0035

>NTDB_id=32908 BAMF_RS21720 WP_013350919.1 275980..277101(+) (rapF) [Bacillus amyloliquefaciens DSM 7 = ATCC 23350]
MKSKIPSEEVAVKMNEWYKLIRAFEADKAEALKREIQYELEDMEENQDLLLYFSLMEFRHRIMLDKLKPMKDGDCQPPFS
EMLTEIEMNQQKLTGLLEYYFYYFRGMYEFKQKNFISAISHYKRAEEKLEYVEDDIEKAEFLYKISEVYYHIKQTFFSMN
YAGQALDIYKKYELYGMRRVQCKFIIAGNLMDAQQHEKALEYLECALEHAERLDDHYMIAAAYYNIGHCMYSLGDFCKAA
EDFERAAAGFEAHSFQQVIQAVFSLTHIYFKTERYSDAAASYARGMKSAAEWEDEIYMTKFRLIKELYQGDGGQDSLKEY
FDILESKELLADTEELLQDAAHFFHKRENYKAASYFFSRLVGVQKRLQMNNYA

Nucleotide

• Length: 1122 bp

>NTDB_id=32908 BAMF_RS21720 WP_013350919.1 275980..277101(+) (rapF) [Bacillus amyloliquefaciens DSM 7 = ATCC 23350]
ATGAAATCCAAGATTCCATCTGAAGAAGTAGCCGTAAAGATGAATGAATGGTATAAGCTGATCCGCGCGTTTGAAGCCGA
TAAAGCTGAAGCGCTGAAGCGGGAGATTCAATATGAACTCGAAGATATGGAAGAAAATCAGGATTTATTATTGTATTTTT
CATTGATGGAATTTAGGCATCGCATCATGCTGGATAAATTAAAACCCATGAAAGACGGCGACTGCCAGCCTCCGTTCTCT
GAAATGCTCACTGAGATTGAGATGAACCAGCAAAAGCTGACCGGCTTACTGGAATATTATTTTTACTATTTCAGGGGAAT
GTATGAATTTAAGCAGAAAAACTTCATCTCAGCGATTTCTCATTATAAACGGGCTGAGGAAAAACTAGAATATGTGGAAG
ATGACATAGAGAAAGCCGAGTTTCTGTATAAAATTTCTGAAGTGTACTATCATATCAAGCAGACTTTCTTTTCGATGAAT
TACGCGGGACAGGCGCTTGATATTTACAAGAAATATGAATTATACGGAATGAGAAGGGTGCAGTGCAAATTTATCATTGC
CGGTAACCTGATGGACGCCCAGCAGCATGAAAAAGCGCTGGAATACCTTGAATGCGCCTTGGAGCACGCGGAGCGGCTTG
ATGATCACTATATGATTGCTGCGGCCTATTATAATATCGGGCACTGTATGTACAGTTTGGGTGACTTTTGCAAGGCGGCG
GAAGACTTTGAACGAGCGGCTGCCGGTTTTGAAGCGCACAGCTTCCAGCAGGTCATCCAGGCTGTTTTTTCACTCACGCA
TATCTACTTTAAAACGGAAAGATACAGTGACGCCGCCGCCAGCTATGCGCGGGGCATGAAAAGCGCGGCGGAGTGGGAAG
ACGAGATCTATATGACAAAATTCCGTCTGATTAAGGAGCTTTATCAGGGTGACGGCGGACAGGATTCGCTCAAAGAGTAT
TTTGACATTCTGGAATCAAAGGAGCTGTTGGCCGACACTGAGGAACTGCTTCAGGACGCGGCTCATTTCTTTCATAAACG
GGAGAACTACAAAGCGGCATCTTATTTTTTCAGCAGGCTTGTCGGCGTGCAAAAAAGGCTGCAAATGAACAATTACGCAT
AA

Domains

Predicted by InterProScan.

• response regulator aspartate phosphatase H, N terminal (RapH_N) (4-65)

• Tetratricopeptide repeat (TPR_12) (187-251)

3D structure

• AlphaFold DB: A0A9P1NGF1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
rapF	Bacillus subtilis subsp. subtilis str. 168	42.588	99.464	0.424
rapC	Bacillus subtilis subsp. subtilis str. 168	40.323	99.732	0.402