Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   MCCL_RS10540 Genome accession   NC_011999
Coordinates   2019224..2020585 (-) Length   453 a.a.
NCBI ID   WP_015912383.1    Uniprot ID   B9E8S3
Organism   Macrococcoides caseolyticum JCSC5402     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2014224..2025585
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MCCL_RS10515 (MCCL_1879) cysE 2014651..2015304 (-) 654 WP_101038220.1 serine O-acetyltransferase -
  MCCL_RS10520 (MCCL_1880) gltX 2015541..2016995 (-) 1455 WP_015912379.1 glutamate--tRNA ligase -
  MCCL_RS10525 (MCCL_1881) ispF 2017013..2017492 (-) 480 WP_015912380.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  MCCL_RS10530 (MCCL_1882) ispD 2017489..2018169 (-) 681 WP_015912381.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  MCCL_RS10535 (MCCL_1883) - 2018162..2019211 (-) 1050 WP_015912382.1 PIN/TRAM domain-containing protein -
  MCCL_RS10540 (MCCL_1884) radA 2019224..2020585 (-) 1362 WP_015912383.1 DNA repair protein RadA Machinery gene
  MCCL_RS10545 (MCCL_1885) - 2020810..2023238 (-) 2429 Protein_2122 ATP-dependent Clp protease ATP-binding subunit -
  MCCL_RS10550 (MCCL_1887) - 2023248..2024252 (-) 1005 WP_015912386.1 protein arginine kinase -
  MCCL_RS10555 (MCCL_1888) - 2024242..2024628 (-) 387 WP_015912387.1 UvrB/UvrC motif-containing protein -
  MCCL_RS10560 (MCCL_1889) - 2024639..2025100 (-) 462 WP_041636223.1 CtsR family transcriptional regulator -

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 50240.85 Da        Isoelectric Point: 7.2379

>NTDB_id=32898 MCCL_RS10540 WP_015912383.1 2019224..2020585(-) (radA) [Macrococcoides caseolyticum JCSC5402]
MAKVKSTFECMACGYQSPKWMGKCPNCGAWNQMEEVIEHKQKGPKNAISESQSDNKVEKLKDITKESVPRDHTQMKELDR
VLGGGIVPGSLILIGGDPGIGKSTLLLQVCAMLSQNHPVLYISGEESVRQTKLRADRLLEDAGELDVYAETNLQIIHETV
KRSKPKFLVIDSIQTIFHPEVTSAPGSVSQVRECTQELMRIAKQMNIATFIVGHVTKEGQIAGPRLLEHMVDTVLYFEGD
THHSYRILRAVKNRFGSTNEMGIFEMKNTGLKEVLNPSEMFLEERTKNVAGSTIVATMEGTRPLLVEVQSLVTPTSFHNP
RRMASGVDHNRLNLLMAVLEKKQGYLLQQQDAYVKVAGGVKLDEPAVDLSVIVSIASSYNDKPTRGDDCFIGEVGLTGEV
RRVARIEQRVQEAEKLGFKRVIIPKNNIGGWDFPGNIEVIGVTNINEALKMAF

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=32898 MCCL_RS10540 WP_015912383.1 2019224..2020585(-) (radA) [Macrococcoides caseolyticum JCSC5402]
TTGGCAAAGGTGAAAAGTACATTTGAATGTATGGCCTGTGGTTATCAGTCACCGAAATGGATGGGAAAATGTCCGAATTG
TGGTGCATGGAATCAAATGGAAGAAGTTATTGAACATAAACAGAAGGGTCCTAAAAATGCGATTTCTGAATCACAAAGTG
ATAATAAAGTTGAAAAACTAAAAGATATAACGAAAGAAAGTGTTCCTCGTGATCATACGCAGATGAAAGAACTAGATCGT
GTACTTGGAGGAGGAATCGTTCCCGGTTCATTGATACTTATCGGTGGAGACCCAGGGATTGGGAAATCCACATTACTACT
ACAAGTTTGTGCAATGCTTTCACAAAACCATCCCGTACTTTATATTTCTGGAGAGGAATCGGTAAGACAAACGAAACTAC
GTGCCGATCGTCTGTTAGAGGATGCAGGGGAGCTTGATGTCTATGCAGAAACCAACCTTCAGATTATTCACGAAACGGTA
AAACGATCGAAACCGAAATTTTTAGTTATCGATTCAATTCAAACAATATTTCATCCAGAGGTTACAAGTGCACCTGGTTC
TGTATCGCAAGTTAGAGAATGTACACAAGAGTTAATGCGTATTGCAAAGCAGATGAACATTGCAACATTTATCGTAGGGC
ACGTAACAAAAGAAGGACAAATTGCAGGTCCAAGGCTACTAGAACATATGGTTGATACAGTTCTTTACTTTGAAGGAGAC
ACTCATCACAGCTACAGAATATTACGCGCGGTGAAGAATCGTTTTGGTTCGACAAATGAAATGGGTATATTTGAAATGAA
GAATACAGGCCTTAAAGAAGTGCTGAACCCTTCAGAAATGTTCCTTGAAGAACGTACAAAAAATGTAGCGGGTTCAACTA
TCGTTGCAACGATGGAAGGCACGCGTCCATTACTAGTCGAAGTTCAGTCACTTGTAACGCCGACATCCTTTCATAATCCA
AGAAGAATGGCATCAGGTGTGGATCATAATCGTCTGAATTTACTGATGGCTGTACTAGAGAAAAAACAAGGATATTTATT
ACAGCAGCAGGATGCATATGTTAAGGTTGCGGGTGGTGTCAAACTTGATGAACCTGCTGTTGACTTAAGTGTAATCGTAA
GCATTGCTTCAAGCTATAACGATAAGCCGACACGAGGAGATGACTGTTTTATCGGAGAAGTCGGTCTGACTGGTGAAGTG
AGACGTGTTGCGCGTATTGAACAACGTGTACAAGAAGCTGAAAAACTCGGTTTTAAACGTGTTATCATCCCCAAAAATAA
TATTGGTGGCTGGGATTTCCCTGGAAATATAGAAGTAATCGGTGTAACTAATATTAACGAAGCATTAAAAATGGCATTTT
AA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(343-427)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(66-219)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B9E8S3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

66.447

100

0.669
  radA Streptococcus pneumoniae Rx1

62.996

100

0.631
  radA Streptococcus pneumoniae D39

62.996

100

0.631
  radA Streptococcus pneumoniae R6

62.996

100

0.631
  radA Streptococcus pneumoniae TIGR4

62.996

100

0.631
  radA Streptococcus mitis NCTC 12261

62.996

100

0.631
  radA Streptococcus mitis SK321

62.996

100

0.631

Multiple sequence alignment