Detailed information    

insolico Bioinformatically predicted

Overview

Name   priA   Type   Machinery gene
Locus tag   N032_RS02215 Genome accession   NZ_CP034078
Coordinates   443392..445611 (-) Length   739 a.a.
NCBI ID   WP_003422549.1    Uniprot ID   -
Organism   Pseudomonas syringae pv. pisi str. PP1     
Function   DNA puliing through the inner membrane (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 438392..450611
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N032_RS02195 (N032_02195) hslU 438512..439849 (-) 1338 WP_003316641.1 ATP-dependent protease ATPase subunit HslU -
  N032_RS02200 (N032_02200) hslV 439916..440446 (-) 531 WP_003392345.1 ATP-dependent protease subunit HslV -
  N032_RS02205 (N032_02205) - 440861..441556 (-) 696 WP_003403056.1 SPOR domain-containing protein -
  N032_RS02210 (N032_02210) argS 441558..443294 (-) 1737 WP_024650482.1 arginine--tRNA ligase -
  N032_RS02215 (N032_02215) priA 443392..445611 (-) 2220 WP_003422549.1 primosomal protein N' Machinery gene
  N032_RS02220 (N032_02220) rpmE 445781..446002 (+) 222 WP_002551648.1 50S ribosomal protein L31 -
  N032_RS02225 (N032_02225) - 446016..446819 (+) 804 WP_024650483.1 thermonuclease family protein -
  N032_RS02230 (N032_02230) - 447175..448443 (+) 1269 WP_003316648.1 malic enzyme-like NAD(P)-binding protein -

Sequence

Protein


Download         Length: 739 a.a.        Molecular weight: 81901.20 Da        Isoelectric Point: 8.4231

>NTDB_id=328758 N032_RS02215 WP_003422549.1 443392..445611(-) (priA) [Pseudomonas syringae pv. pisi str. PP1]
MPDAILRLALPSPLRRLFDYRAPAGVSRSALQPGMRLRVPFGRREMIGILVEVVDHSEVPADKLKPAIALLDSEAPLPPA
LFKLCLWTSQYYQHSLGDTLSWALPVLLRQGELAESRQERFWHVTPGASVDDPRIARAPKQREALTTLAQHPHGVAHQLL
SKLMLNKDSLNLLLAKELVYVEVRSHAPSARHEHWLAQPELPLNTEQRAAYEAIRAGFDSFHAFLLAGVTGSGKTEVYLQ
LIRETLEAGKQALVLIPEINLGPQTLARFEQRFNARIALVHSAVNDRERLDAWLAARDGEADIIIGTRSALFTPMKNPGL
IIIDEEHDGSYKQQEGLRYHARDLALVRARQENIPIVLGSATPSLESLHNAYTGRYGLLRLNERAGGAQQPRFMRLDVKS
RPLDSGISGPMQQAIGQTLAAGQQVLVFLNRRGFAPTLLCHDCGWMSGCQRCDARMTVHQRSGELRCHHCGYVERVPRQC
PSCGKVDLRPVGAGTERAEERLAILFPDYPVLRVDRDSTSRKDAMNQLFTTIQRGQPCILVGTQMLAKGHHFPRVTLVSI
LDADGGLFSGDFRASERMAQLIVQVAGRAGRAEEPGKVIIQTHLADHPLLIQLTEQGYFAFAEQALSERRSAGLPPFSHL
ALLRAEAHKPGQAEAFLDQACSDAEQLVAQMALGGIELLGPVPAPMERRAGRYRAQLLVQSSARAPLHKLLATWLLALEQ
MPSGRQVRWSLDVDPVDLY

Nucleotide


Download         Length: 2220 bp        

>NTDB_id=328758 N032_RS02215 WP_003422549.1 443392..445611(-) (priA) [Pseudomonas syringae pv. pisi str. PP1]
GTGCCCGACGCCATCCTGCGCCTTGCCCTGCCCTCGCCGCTTCGCCGCCTGTTCGACTACCGCGCCCCAGCGGGCGTGTC
GCGCAGCGCCTTGCAGCCGGGCATGCGTCTGCGAGTGCCGTTCGGACGCCGGGAAATGATCGGCATTCTGGTGGAAGTGG
TCGATCACAGCGAAGTGCCCGCCGACAAGCTCAAGCCGGCGATCGCCCTGCTCGACAGCGAAGCGCCGCTGCCACCTGCG
CTGTTCAAGCTGTGCCTGTGGACCTCGCAGTACTACCAGCACAGCCTCGGAGACACACTGAGCTGGGCCTTGCCGGTGCT
GTTGCGTCAGGGCGAACTGGCCGAAAGTCGCCAGGAACGCTTCTGGCACGTGACCCCGGGCGCCAGCGTCGACGATCCGC
GCATCGCCCGCGCACCCAAGCAGCGCGAAGCCCTGACCACCCTGGCCCAGCACCCGCACGGGGTGGCGCATCAACTGCTC
AGCAAGCTGATGCTTAACAAGGACAGCCTCAACCTGTTGCTGGCCAAGGAGCTGGTGTACGTCGAGGTCCGCAGCCATGC
GCCCAGCGCACGCCATGAACACTGGCTGGCCCAGCCTGAATTGCCGCTCAACACCGAACAACGCGCCGCTTACGAAGCGA
TCCGCGCCGGGTTCGACAGCTTTCATGCGTTTCTGCTGGCAGGCGTCACCGGCAGCGGCAAGACTGAAGTCTATCTGCAA
CTGATCCGCGAAACCCTCGAAGCCGGCAAGCAGGCGCTGGTGCTGATCCCGGAAATCAACCTCGGCCCGCAGACCCTGGC
GCGCTTCGAGCAACGCTTCAATGCGCGCATTGCGCTGGTCCACTCGGCGGTCAACGACCGCGAACGGCTGGATGCCTGGC
TGGCAGCTCGCGATGGCGAGGCGGACATCATCATCGGCACGCGTTCGGCGCTGTTCACGCCGATGAAAAACCCCGGCCTG
ATCATCATCGACGAAGAACACGACGGCTCCTATAAACAGCAGGAAGGTCTGCGCTATCACGCCCGCGATCTGGCCTTGGT
GCGGGCGCGTCAGGAAAACATTCCGATTGTCCTTGGCTCGGCCACACCGTCCCTGGAAAGCCTGCACAACGCTTACACCG
GCCGCTACGGTCTGCTGCGCCTGAATGAGCGCGCGGGCGGCGCACAGCAGCCGCGTTTCATGCGCCTGGACGTCAAGAGC
CGACCGCTGGACAGCGGCATCTCCGGCCCCATGCAGCAGGCCATCGGCCAGACGCTCGCGGCAGGCCAGCAGGTCTTGGT
GTTTCTCAACCGTCGCGGCTTTGCGCCAACCTTGCTGTGCCACGACTGCGGCTGGATGTCCGGTTGCCAACGCTGCGATG
CACGCATGACCGTGCACCAGCGCTCGGGCGAACTGCGCTGCCATCACTGCGGGTATGTCGAGCGTGTACCGCGTCAGTGT
CCGTCGTGCGGCAAGGTGGATTTACGCCCGGTAGGCGCCGGCACCGAGCGCGCCGAAGAGCGTCTGGCGATTCTGTTTCC
GGACTACCCCGTACTGCGTGTCGACCGCGACAGCACGTCGCGCAAGGACGCCATGAACCAGCTGTTCACGACGATCCAGC
GCGGTCAGCCATGCATTCTGGTCGGCACGCAAATGCTTGCCAAGGGCCATCACTTTCCCCGGGTCACGCTGGTCTCGATT
CTGGACGCCGACGGCGGGCTGTTTTCCGGCGACTTCCGCGCCAGCGAGCGCATGGCGCAATTGATCGTGCAGGTGGCAGG
TCGTGCCGGGCGCGCCGAAGAGCCTGGCAAGGTGATCATCCAGACCCACCTGGCCGACCACCCACTGCTGATCCAGCTGA
CCGAGCAGGGTTATTTCGCCTTTGCCGAACAGGCCTTGAGCGAGCGCCGTTCGGCGGGTCTGCCACCGTTTTCACACCTC
GCACTGCTGCGCGCTGAAGCGCACAAGCCGGGACAGGCCGAGGCCTTTCTCGATCAGGCCTGCAGCGATGCGGAGCAACT
GGTCGCGCAGATGGCGCTGGGCGGCATCGAGCTGCTGGGCCCGGTTCCGGCACCGATGGAACGCCGCGCCGGGCGTTATC
GTGCGCAACTGCTGGTGCAGTCCAGCGCCCGCGCACCCTTGCATAAATTGCTTGCCACCTGGCTGCTCGCGCTGGAGCAA
ATGCCCAGCGGTCGGCAGGTCAGATGGTCGCTGGATGTGGACCCTGTGGATCTTTATTAA

Domains

Predicted by InterproScan.

Primosomal protein N C-terminal domain (PriA_C)

(636-735)

DEAD/DEAH box helicase (DEAD)

(207-367)

3DNA-binding domain (3BD) (PriA_3primeBD)

(9-105)

PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA)

(449-475)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  priA Acinetobacter baumannii D1279779

47.844

100

0.48

Multiple sequence alignment