Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   EG219_RS08250 Genome accession   NZ_CP034037
Coordinates   1673539..1673973 (+) Length   144 a.a.
NCBI ID   WP_015417516.1    Uniprot ID   -
Organism   Bacillus velezensis strain BCSo1     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1668539..1678973
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EG219_RS08245 (EG219_08245) - 1672039..1673250 (-) 1212 WP_007409852.1 cytochrome P450 -
  EG219_RS08250 (EG219_08250) nucA/comI 1673539..1673973 (+) 435 WP_015417516.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  EG219_RS08255 (EG219_08255) - 1674033..1674788 (+) 756 WP_003154084.1 YoaK family protein -
  EG219_RS08260 (EG219_08260) - 1674822..1675184 (-) 363 WP_007410383.1 hypothetical protein -
  EG219_RS08265 (EG219_08265) - 1675376..1676704 (-) 1329 WP_007410382.1 S8 family peptidase -
  EG219_RS08270 (EG219_08270) - 1676883..1677116 (+) 234 WP_032865298.1 hypothetical protein -
  EG219_RS08275 (EG219_08275) - 1677372..1678079 (+) 708 WP_007410381.1 poly-gamma-glutamate hydrolase family protein -
  EG219_RS08280 (EG219_08280) - 1678139..1678591 (+) 453 WP_007410380.1 OsmC family protein -
  EG219_RS08285 (EG219_08285) - 1678605..1678958 (-) 354 WP_007410379.1 multidrug efflux SMR transporter -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 15514.44 Da        Isoelectric Point: 7.2418

>NTDB_id=328538 EG219_RS08250 WP_015417516.1 1673539..1673973(+) (nucA/comI) [Bacillus velezensis strain BCSo1]
MNAFMKWAASLLLVISLQFGLTGAGIHSDSAARAASRYDQVLYFPLSKYPETGNHIKDAISAGHSEICTIDRGGAENRRK
ESLKGIPTKPGFDRDEWPMAVCTEGGAGADVRYVTPSDNRGAGSWVGNQMSGYSDGTRVLFIVQ

Nucleotide


Download         Length: 435 bp        

>NTDB_id=328538 EG219_RS08250 WP_015417516.1 1673539..1673973(+) (nucA/comI) [Bacillus velezensis strain BCSo1]
ATGAATGCGTTTATGAAATGGGCGGCAAGTCTGCTTTTGGTGATCTCTCTTCAGTTCGGCCTTACGGGCGCCGGCATCCA
TTCGGACAGTGCTGCTCGTGCCGCATCCCGATACGATCAGGTGCTGTATTTCCCGCTGTCAAAATATCCGGAGACGGGAA
ATCATATAAAAGACGCCATTTCGGCAGGTCATTCTGAGATTTGTACAATTGATCGGGGCGGAGCGGAGAATAGAAGGAAA
GAATCATTGAAAGGAATTCCGACAAAGCCCGGGTTTGACCGTGATGAATGGCCCATGGCAGTCTGCACAGAAGGCGGGGC
GGGGGCTGATGTCAGATATGTAACCCCGTCGGATAACCGCGGGGCCGGTTCATGGGTTGGAAATCAAATGAGCGGATATT
CCGACGGCACGAGAGTATTATTTATCGTTCAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-138)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

57.258

86.111

0.493

Multiple sequence alignment