Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   EFD78_RS08205 Genome accession   NZ_CP033977
Coordinates   1661586..1662560 (-) Length   324 a.a.
NCBI ID   WP_000697220.1    Uniprot ID   W8U5L8
Organism   Staphylococcus aureus strain P2D15C1     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1656586..1667560
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EFD78_RS08165 (EFD78_08525) gcvT 1657087..1658178 (-) 1092 WP_000093349.1 glycine cleavage system aminomethyltransferase GcvT -
  EFD78_RS08170 (EFD78_08530) - 1658337..1658861 (-) 525 WP_001015120.1 shikimate kinase -
  EFD78_RS08175 (EFD78_08535) - 1658851..1658997 (-) 147 WP_001792109.1 hypothetical protein -
  EFD78_RS08180 (EFD78_08540) comGF 1659094..1659591 (-) 498 WP_001788897.1 competence type IV pilus minor pilin ComGF Machinery gene
  EFD78_RS08185 (EFD78_08545) comGE 1659509..1659808 (-) 300 WP_000844410.1 hypothetical protein Machinery gene
  EFD78_RS08190 (EFD78_08550) comGD 1659795..1660241 (-) 447 WP_001788899.1 competence type IV pilus minor pilin ComGD Machinery gene
  EFD78_RS08195 (EFD78_08555) comGC 1660219..1660530 (-) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  EFD78_RS08200 (EFD78_08560) comGB 1660544..1661614 (-) 1071 WP_000776422.1 competence type IV pilus assembly protein ComGB Machinery gene
  EFD78_RS08205 (EFD78_08565) comGA 1661586..1662560 (-) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  EFD78_RS08210 (EFD78_08570) - 1662612..1663235 (-) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  EFD78_RS08215 (EFD78_08575) - 1663232..1663561 (-) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  EFD78_RS08220 (EFD78_08580) - 1663561..1664547 (-) 987 WP_000161311.1 ROK family glucokinase -
  EFD78_RS08225 (EFD78_08585) - 1664544..1664747 (-) 204 WP_000087561.1 YqgQ family protein -
  EFD78_RS08230 (EFD78_08590) - 1664728..1666191 (-) 1464 WP_001017852.1 rhomboid family protein -
  EFD78_RS08235 (EFD78_08595) - 1666203..1666742 (-) 540 WP_000164546.1 5-formyltetrahydrofolate cyclo-ligase -
  EFD78_RS08240 (EFD78_08600) rpmG 1666951..1667100 (-) 150 WP_001265709.1 50S ribosomal protein L33 -

Sequence

Protein


Download         Length: 324 a.a.        Molecular weight: 36897.96 Da        Isoelectric Point: 8.8516

>NTDB_id=327554 EFD78_RS08205 WP_000697220.1 1661586..1662560(-) (comGA) [Staphylococcus aureus strain P2D15C1]
MKILFQEIINKAIEMKASDVHFIPVKNEVSIKFRINDNLEQYEQIGNSIYQKLLVYMKFQAGLDVSTQQVAQSGRYSYHF
NKIYFLRISTLPLSLGQESCVIRIVPQFFQQQKSTYKFNDFKHLMNKKQGLLLFSGPTGSGKSTLMYQMVSYANKALNLN
VISIEDPVEMQIPGIVQINVNDKAGINYVNSFKAILRCDPDVILIGEIRDKDVAKCVIQASLSGHLVLTTLHATDCKGAI
LRLLEMGISVQELIQATNLIINQRLVTTIKQQRQLVCEILSQQQLRYFFSHNHSLPSSFKNLEDKLDDMTKAGVICETTM
DKYI

Nucleotide


Download         Length: 975 bp        

>NTDB_id=327554 EFD78_RS08205 WP_000697220.1 1661586..1662560(-) (comGA) [Staphylococcus aureus strain P2D15C1]
TTGAAGATTCTATTTCAAGAAATAATTAATAAAGCGATAGAAATGAAAGCGAGTGATGTACATTTTATTCCAGTTAAAAA
TGAAGTAAGTATTAAATTTAGAATTAATGATAACTTGGAGCAGTATGAACAAATTGGGAATAGCATTTATCAAAAGTTAT
TAGTTTATATGAAGTTTCAAGCTGGGCTTGATGTTTCTACACAGCAAGTCGCACAGAGCGGTCGATATAGTTACCATTTC
AATAAAATATATTTTTTGAGAATATCAACTTTACCATTGTCACTTGGCCAAGAAAGTTGTGTTATCAGAATTGTACCTCA
ATTTTTTCAACAACAGAAATCAACTTATAAATTCAATGATTTTAAACACCTCATGAATAAGAAACAAGGATTACTATTGT
TTAGTGGGCCAACTGGTTCAGGAAAGAGTACATTAATGTATCAAATGGTCTCATACGCGAATAAAGCCTTGAATTTAAAT
GTAATTTCTATAGAGGATCCTGTAGAGATGCAAATTCCTGGTATCGTCCAAATTAATGTGAATGATAAAGCTGGCATTAA
CTATGTAAATTCGTTTAAAGCTATTTTAAGATGTGATCCTGATGTTATTTTAATAGGTGAAATCAGAGATAAAGATGTTG
CCAAGTGTGTTATACAGGCTAGTTTAAGTGGTCACCTTGTTCTGACTACATTGCATGCAACTGATTGTAAAGGTGCTATT
TTAAGGCTATTAGAAATGGGCATTTCTGTACAAGAATTGATACAGGCAACTAACTTAATTATAAACCAACGACTTGTAAC
TACTATTAAGCAACAGCGACAATTAGTATGCGAAATTCTATCTCAGCAACAACTCCGATATTTCTTTTCCCATAATCATT
CATTACCATCATCATTTAAGAACTTAGAAGATAAACTTGATGATATGACAAAAGCAGGTGTCATTTGTGAAACTACAATG
GATAAATACATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB W8U5L8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Staphylococcus aureus MW2

100

100

1
  comGA Staphylococcus aureus N315

100

100

1

Multiple sequence alignment