Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   EHF44_RS12955 Genome accession   NZ_CP033969
Coordinates   2460843..2462210 (+) Length   455 a.a.
NCBI ID   WP_124684110.1    Uniprot ID   A0A3G8H1L5
Organism   Cupriavidus pauculus strain FDAARGOS_614     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2455843..2467210
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EHF44_RS12935 (EHF44_12935) - 2455892..2456806 (+) 915 WP_124684106.1 uracil-DNA glycosylase family protein -
  EHF44_RS12940 (EHF44_12940) - 2456781..2457872 (+) 1092 WP_124684107.1 DUF1853 family protein -
  EHF44_RS12945 (EHF44_12945) lplT 2457890..2459209 (-) 1320 WP_124684108.1 lysophospholipid transporter LplT -
  EHF44_RS12950 (EHF44_12950) alr 2459681..2460802 (+) 1122 WP_124684109.1 alanine racemase -
  EHF44_RS12955 (EHF44_12955) radA 2460843..2462210 (+) 1368 WP_124684110.1 DNA repair protein RadA Machinery gene
  EHF44_RS12960 (EHF44_12960) - 2462334..2462819 (+) 486 WP_124684111.1 disulfide bond formation protein B -
  EHF44_RS12965 (EHF44_12965) - 2462962..2464887 (-) 1926 WP_124684112.1 ATP-binding cassette domain-containing protein -
  EHF44_RS12970 (EHF44_12970) - 2465176..2466078 (+) 903 WP_124684113.1 MurR/RpiR family transcriptional regulator -
  EHF44_RS12975 (EHF44_12975) - 2466105..2467073 (+) 969 WP_124684114.1 isoaspartyl peptidase/L-asparaginase family protein -

Sequence

Protein


Download         Length: 455 a.a.        Molecular weight: 48154.25 Da        Isoelectric Point: 7.1653

>NTDB_id=327521 EHF44_RS12955 WP_124684110.1 2460843..2462210(+) (radA) [Cupriavidus pauculus strain FDAARGOS_614]
MAKTKTVYTCTECGGTAPRWAGQCPQCNAWNTLVETVADSGSSAARRFQPLAASAVVRKLSDIEAADVPRFTTGIDEFDR
VLGGGLVSGGVVLIGGDPGIGKSTLLLQTLANLSASRRVLYVSGEESGAQIALRAHRLGVEAASLGLLAEIQLEKIQAAL
EADKPEVAVIDSIQTLYSDALTSAPGSVAQVRECAAQLTRIAKSSGTTIILVGHVTKEGSLAGPRVLEHIVDTVLYFEGD
THSSHRLIRAFKNRFGAVNELGVFAMTERGLRGISNPSALFLSQHAEQVAGSCVLVTQEGTRPLLVEVQALVDTANVPNP
RRLAVGLEQNRLAMLLAVLHRHAGIACFDQDVFLNAVGGVKISEPAADLAVLLSIHSSMRNKPLPRGLVVFGEVGLAGEI
RPSPRGQERLREAAKLGFTTAVIPKANAPKQAIDGLEVIAVDRLEQAIDRVRQLD

Nucleotide


Download         Length: 1368 bp        

>NTDB_id=327521 EHF44_RS12955 WP_124684110.1 2460843..2462210(+) (radA) [Cupriavidus pauculus strain FDAARGOS_614]
ATGGCCAAGACCAAGACCGTCTACACCTGTACCGAATGCGGCGGCACCGCGCCGCGCTGGGCGGGCCAGTGCCCGCAATG
CAACGCCTGGAACACACTTGTCGAAACCGTGGCCGACTCGGGCTCGTCGGCCGCGCGGCGCTTCCAGCCGCTGGCGGCGT
CGGCCGTGGTCCGCAAGCTGAGCGATATCGAAGCGGCCGACGTGCCGCGCTTCACCACCGGCATCGACGAGTTCGATCGC
GTGCTGGGCGGCGGGCTGGTGTCCGGCGGGGTGGTGCTGATTGGCGGCGACCCCGGCATCGGCAAGTCCACGCTGCTGTT
GCAGACGCTGGCCAACCTGTCGGCCAGCCGGCGCGTGCTGTACGTGAGCGGCGAGGAATCGGGCGCCCAGATCGCGTTGC
GCGCCCACCGGCTGGGCGTGGAGGCGGCCTCGCTGGGCCTGCTGGCCGAGATCCAGCTGGAGAAGATCCAGGCCGCGCTG
GAGGCCGACAAGCCCGAGGTGGCCGTGATCGACTCGATCCAGACGCTGTACTCGGACGCGCTGACGTCGGCGCCGGGCTC
GGTGGCCCAGGTGCGCGAATGCGCGGCGCAACTGACGCGCATTGCCAAGAGCAGCGGCACGACCATCATCCTCGTCGGCC
ACGTGACCAAGGAAGGCAGCCTGGCCGGGCCGCGCGTGCTCGAACATATCGTCGATACGGTGCTGTACTTCGAGGGCGAC
ACCCATTCGTCGCACCGGCTGATCCGTGCGTTCAAGAACCGCTTTGGCGCGGTCAACGAGCTTGGCGTTTTTGCGATGAC
CGAGCGCGGGCTGCGCGGCATCAGCAATCCGTCGGCGTTGTTTCTGTCGCAGCATGCCGAGCAGGTGGCCGGCTCGTGCG
TGCTGGTGACGCAGGAGGGCACGCGGCCGCTGCTGGTGGAGGTGCAGGCGCTGGTGGATACGGCCAACGTGCCGAACCCG
CGCCGGCTGGCCGTGGGGCTGGAGCAGAACCGGCTGGCGATGCTGCTGGCGGTGCTGCACCGGCACGCGGGCATCGCGTG
CTTCGACCAGGACGTGTTTCTGAACGCGGTGGGCGGGGTCAAGATCAGCGAACCGGCGGCCGACCTGGCCGTGCTGCTGT
CGATCCATTCGTCGATGCGCAACAAGCCGCTGCCGCGCGGGCTGGTGGTGTTCGGCGAGGTGGGGCTGGCCGGCGAGATC
CGGCCCAGCCCGCGCGGGCAGGAGCGGCTGCGCGAGGCGGCCAAGCTCGGTTTCACGACGGCCGTGATTCCGAAGGCCAA
CGCACCCAAGCAGGCCATCGACGGGCTGGAAGTGATTGCCGTGGACCGCCTGGAGCAAGCCATCGACCGCGTGCGGCAAC
TGGACTGA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(66-219)

Lon protease (S16) C-terminal proteolytic domain (Lon_C)

(359-449)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3G8H1L5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

50.766

100

0.51
  radA Streptococcus pneumoniae Rx1

46.104

100

0.468
  radA Streptococcus pneumoniae D39

46.104

100

0.468
  radA Streptococcus pneumoniae R6

46.104

100

0.468
  radA Streptococcus pneumoniae TIGR4

46.104

100

0.468
  radA Streptococcus mitis SK321

46.204

100

0.468
  radA Streptococcus mitis NCTC 12261

45.887

100

0.466

Multiple sequence alignment