Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   EHF44_RS03860 Genome accession   NZ_CP033969
Coordinates   474151..475632 (+) Length   493 a.a.
NCBI ID   WP_124682529.1    Uniprot ID   A0A3G8GWI4
Organism   Cupriavidus pauculus strain FDAARGOS_614     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 469151..480632
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EHF44_RS03840 (EHF44_03840) - 470856..471839 (+) 984 WP_124682525.1 SIS domain-containing protein -
  EHF44_RS03845 (EHF44_03845) lptC 471843..472472 (+) 630 WP_124682526.1 LPS export ABC transporter periplasmic protein LptC -
  EHF44_RS03850 (EHF44_03850) lptA 472537..473184 (+) 648 WP_124682527.1 lipopolysaccharide transport periplasmic protein LptA -
  EHF44_RS03855 (EHF44_03855) lptB 473206..474006 (+) 801 WP_124682528.1 LPS export ABC transporter ATP-binding protein -
  EHF44_RS03860 (EHF44_03860) rpoN/rpoN1 474151..475632 (+) 1482 WP_124682529.1 RNA polymerase factor sigma-54 Machinery gene
  EHF44_RS03865 (EHF44_03865) raiA 475683..476036 (+) 354 WP_019449655.1 ribosome-associated translation inhibitor RaiA -
  EHF44_RS03870 (EHF44_03870) ptsN 476512..476967 (+) 456 WP_066739687.1 PTS IIA-like nitrogen regulatory protein PtsN -
  EHF44_RS03875 (EHF44_03875) hprK 477035..478006 (+) 972 WP_124682530.1 HPr(Ser) kinase/phosphatase -
  EHF44_RS03880 (EHF44_03880) - 478195..478530 (+) 336 WP_124682531.1 PsiF family protein -
  EHF44_RS03885 (EHF44_03885) rapZ 478657..479547 (+) 891 WP_124682532.1 RNase adapter RapZ -
  EHF44_RS03890 (EHF44_03890) - 479604..480257 (+) 654 WP_124682533.1 LON peptidase substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 493 a.a.        Molecular weight: 54951.28 Da        Isoelectric Point: 4.9205

>NTDB_id=327511 EHF44_RS03860 WP_124682529.1 474151..475632(+) (rpoN/rpoN1) [Cupriavidus pauculus strain FDAARGOS_614]
MKPSLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVEQALTENPLLERENDWIESPLRVAADGSVNLQSAPAPAPAEPQ
ANGDDRTDRAAGSEDEGFGDSGSEDYGNDWSLDDFARRPQGDEDEKTPMQLRDADPTLREYLMEQLTPLKISARDKGLAI
FLIESLDDDGYLSASLEEICSELPEELEFEHDEVHAMLTLLQSFDPPGVGARNAAECLSLQLKRLQHPQRELALTIVNQH
LELLAVRDYTRLKKALQVDEIALKAAHELIRSLAPYPGHAYSRPEADFVVPDVFVRKSGSGWIAQLNPDVMPRLRINDMY
AQILRGAKGEAGAAGLQQKLQEARWLIKNIQQRFDTILRVAQAIVERQKNFFSHGEIAMRPLVLREIADTLGLHESTISR
VTTNKYMATPMGTFELKYFFGSHVSTETGGAASSTAIRALIKQLIGAEDPRNPLSDSRIAELLGEQGFVVARRTVAKYRE
ALKIPAVNLRKSL

Nucleotide


Download         Length: 1482 bp        

>NTDB_id=327511 EHF44_RS03860 WP_124682529.1 474151..475632(+) (rpoN/rpoN1) [Cupriavidus pauculus strain FDAARGOS_614]
ATGAAACCTTCGCTTCAGCTCCGACTCTCCCAGCATCTGGCCCTGACGCCGCAACTGCAGCAATCGATTCGGTTGCTGCA
GCTTTCCACGCTCGAACTCCAGCAGGAGGTGGAACAGGCCCTCACGGAAAACCCGCTGCTCGAACGCGAGAACGACTGGA
TCGAAAGCCCGCTGCGGGTGGCCGCGGACGGGTCCGTCAACCTGCAGAGCGCCCCGGCGCCGGCCCCCGCCGAGCCCCAG
GCCAACGGCGACGACCGCACCGATCGCGCCGCTGGCAGCGAGGACGAGGGTTTTGGCGATTCGGGCAGCGAGGACTACGG
CAACGACTGGAGCCTGGACGACTTCGCGCGCCGCCCGCAGGGCGACGAGGACGAGAAGACGCCGATGCAGTTGCGCGACG
CCGATCCCACGCTGCGCGAATACCTGATGGAGCAATTGACGCCCCTCAAGATCTCGGCGCGCGACAAGGGCCTGGCCATC
TTCCTGATCGAATCGCTGGACGACGACGGCTACCTGAGTGCGTCACTCGAGGAAATCTGCAGCGAACTTCCTGAAGAACT
CGAGTTCGAACACGATGAAGTGCACGCGATGCTGACACTGCTGCAGAGCTTCGACCCGCCTGGCGTGGGCGCCCGCAATG
CCGCGGAATGCCTGAGCCTGCAACTGAAGCGGCTGCAGCATCCGCAGCGCGAGCTGGCCCTGACCATCGTCAACCAGCAC
CTGGAACTGCTGGCCGTGCGCGACTACACGCGGCTCAAGAAAGCGCTCCAGGTGGACGAAATAGCATTGAAGGCCGCGCA
CGAGCTGATCCGTTCGCTGGCGCCGTACCCGGGACATGCGTACAGTCGTCCCGAAGCGGATTTCGTGGTGCCGGACGTGT
TCGTGCGCAAGAGCGGCAGCGGCTGGATCGCCCAGTTGAACCCCGACGTGATGCCGCGGCTGCGGATCAACGACATGTAC
GCGCAGATCCTGCGCGGTGCGAAGGGCGAGGCCGGGGCGGCCGGGCTGCAGCAGAAGCTGCAGGAGGCGCGGTGGCTCAT
CAAGAACATCCAGCAGCGATTCGACACGATCCTGCGGGTGGCGCAGGCAATTGTGGAGCGTCAGAAGAACTTTTTTTCGC
ACGGCGAAATAGCGATGCGACCCTTGGTTTTGCGGGAAATAGCCGATACACTCGGTTTACATGAGTCGACCATTTCCCGG
GTGACGACGAACAAATACATGGCCACGCCGATGGGTACTTTCGAGTTGAAGTACTTCTTTGGCAGCCACGTCTCCACCGA
GACGGGCGGCGCGGCCTCATCCACGGCGATCCGCGCGCTGATCAAGCAACTGATAGGAGCCGAAGACCCCAGGAATCCTC
TTTCCGACAGCCGCATCGCCGAATTGCTGGGCGAACAGGGCTTTGTGGTCGCACGCCGCACGGTGGCCAAATACCGCGAA
GCGCTGAAGATCCCCGCAGTGAATCTCCGCAAGTCTTTGTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(336-491)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(130-320)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3G8GWI4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

80.04

100

0.813

Multiple sequence alignment